In vitro study of the penetration of three bacterial strains into root dentine

Pérez, Fabienne; Rochd, Tarik; Lodter, Jean-Philippe; Calas, Paul; Michel, G.

doi:10.1016/0030-4220(93)90302-k

Cited by 51 publications

(45 citation statements)

References 18 publications

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“…Previous light microscopy studies have demonstrated that several gram-negative anaerobic bacterial species, such as Prevotella melananogenica (1) and Prevotella intermedia (37), were unable to invade dentin. On the other hand, it has been reported that Porphyromonas endodontalis BN11a-f and P. gingivalis ATCC 33277 in monoculture were able to invade bovine dentinal tubules, although very few tubules were infected (40).…”

Section: Discussionmentioning

confidence: 99%

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Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

et al. 2000

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Cell wall-anchored polypeptides of the antigen I/II family are produced by many species of oral streptococci. These proteins mediate adhesion of streptococci to salivary glycoproteins and to other oral microorganisms and promote binding of cells to collagen type I and invasion of dentinal tubules. Since infections of the root canal system have a mixed anaerobic bacterial etiology, we investigated the hypothesis that coadhesion of anaerobic bacteria with streptococci may facilitate invasive endodontic disease. Porphyromonas gingivalis ATCC 33277 cells were able to invade dentinal tubules when cocultured with Streptococcus gordonii DL1 (Challis) but not when cocultured with Streptococcus mutans NG8. An isogenic noninvasive mutant of S. gordonii, with production of SspA and SspB (antigen I/II family) polypeptides abrogated, was deficient in binding to collagen and had a 40% reduced ability to support adhesion of P. gingivalis. Heterologous expression of the S. mutans SpaP (antigen I/II) protein in this mutant restored collagen binding and tubule invasion but not adhesion to P. gingivalis or the ability to promote P. gingivalis coinvasion of dentin. An isogenic afimbrial mutant of P. gingivalis had 50% reduced binding to S. gordonii cells but was unaffected in the ability to coinvade dentinal tubules with S. gordonii wild-type cells. Expression of the S. gordonii SspA or SspB polypeptide on the surface of Lactococcus lactis cells endowed these bacteria with the abilities to bind P. gingivalis, penetrate dentinal tubules, and promote P. gingivalis coinvasion of dentin. The results demonstrate that collagen-binding and P. gingivalis-binding properties of antigen I/II polypeptides are discrete functions. Specificity of antigen I/II polypeptide recognition accounts for the ability of P. gingivalis to coinvade dentinal tubules with S. gordonii but not with S. mutans. This provides evidence that the specificity of interbacterial coadhesion may influence directly the etiology of pulpal and periapical diseases.

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Section: Discussionmentioning

confidence: 99%

“…By contrast to results obtained with gram-positive bacteria, monocultures of gram-negative anaerobic bacteria do not invade dentinal tubules (1,37). This has led to the notion that dentinal tubules may in some way be selective for streptococci and other facultatively anaerobic bacteria.…”

mentioning

confidence: 87%

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

et al. 2000

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“…Perez et al (84), using bovine teeth, demonstrated that S. sanguis penetrated 792 m into the tubules. In a follow up study, Perez et al (85) demonstrated that S. sanguis migrated into dentinal tubules but A. naeslundi and Prevotella intermedia did not migrate. Instead, they aggregated and formed clus- …”

Section: In Vitro Studiesmentioning

confidence: 99%

“…Studies with bovine teeth have been questioned because their tubules have wider diameters than human tubules (85). Studies that involve grinding may destroy certain species of bacteria (97).…”

Section: In Vivo Studiesmentioning

confidence: 99%

The Role of Apical Instrumentation in Root Canal Treatment: A Review of the Literature

Baugh

Wallace

2005

Journal of Endodontics

142

113

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The issue of final apical preparation size remains controversial despite considerable clinical and in vitro research. The astute clinician must be aware of this research before choosing any instrumentation system because the informed clinician's decision must be guided by the best available evidenced-based information. This review article generated a Medline-based search strategy to disclose these studies and provides a critique and summary of the results.From the University of Pittsburgh School of Dental Medicine, Pittsburgh, Pennsylvania.Address request for reprints to Dean Baugh, DDS. E-mail address: baughdf@yahoo.com.Copyright © 2005 by the American Association of Endodontists T he most important objective of root canal therapy is to minimize the number of microorganisms and pathologic debris in root canal systems to prevent or treat apical periodontitis. This process of chemomechanical debridement, or cleaning of the root canal systems, has been described as the removal of all of the contents of the root canal systems before and during shaping. Grossman (1) described mechanical cleaning as the most important part of root canal therapy. Schilder (2) also considered cleaning and shaping as the foundation for successful endodontic therapy.Thorough instrumentation of the apical region has long been considered to be an essential component in the cleaning and shaping process. It was discussed as a critical step as early as 1931 by Groove (3). Simon (4) later recognized the apical area as the critical zone for instrumentation. Other authors (5, 6) concluded that the last few millimeters that approach the apical foramen are critical in the instrumentation process. Mechanical instrumentation and irrigation are sound endodontic principles and essential components of successful endodontics (7,8). Research has shown that mechanical instrumentation greatly reduces the number of microorganisms remaining in the root canal system. Mechanical instrumentation (9) has been shown to reduce bacterial count even without irrigants or dressings. A combination of mechanical instrumentation and irrigation (9, 10) further reduced the number of microorganisms by 100 to 1000 times. However, mechanical instrumentation with irrigation does not reliably disinfect an infected root canal system (11-14).Manufacturers developed nickel-titanium rotary instrumentation systems to facilitate the cleaning and shaping process. They are popular because of their apparent ease of use and reduced number of instruments. However, Spangberg (6) noted that the strong emphasis on reducing the number of instruments and limiting apical preparations to small sizes does not produce clean apical preparations in diseased teeth. Given this controversial and important topic, we conducted a broad-based Medline search of the literature to characterize the major factors involved in apical canal instrumentation. Table 1 provides the Medline search strategy used to identify relevant articles for this review. A secondary search was then conducted using the refere...

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“…That is to say, the diameter of the large tubules was almost 10-times that of the dentinal tubules. Furthermore, there are many reports showing that degree of bacterial invasion and localization depends on type of bacterial species in dentin 4,9,16,17) . We have demonstrated that collagen fibrils within large tubules are usually made up of mostly type-1 collagen in human deciduous dentin 3) .…”

Section: Discussionmentioning

confidence: 99%

Relationship between Large Tubules and Dentin Caries in Human Deciduous Tooth

Agematsu

Abe

Shiozaki

et al. 2005

Bull. Tokyo Dent. Coll.

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The purpose of this study was to elucidate the relationship between large tubules and dentin caries by using human deciduous incisors that showed various levels of attrition but no macroscopical lesions resulting from caries. The teeth were cut longitudinally in the mesio-distal direction and the exposed surfaces observed with a high-resolution field emission scanning electron microscope. The inside of each large tubule showed dense collagen fibers running parallel to its long axis and small spherical bodies of aggregated crystals, but no marked attrition. In teeth where attrition had exposed dentin at the incisal edge, oral bacteria had infiltrated the large tubules. Furthermore, in teeth with advanced attrition, it was difficult to distinguish between the large tubules and the surrounding dentin matrix, and numerous bacteria were observed in both areas. These findings support the hypothesis that large tubules play a role in the pathway of caries formation in coronal dentin when incisal dentin is exposed by attrition. This suggests that early treatment of exposed dentin surfaces might be effective in preventing dental caries.

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In vitro study of the penetration of three bacterial strains into root dentine

Cited by 51 publications

References 18 publications

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

The Role of Apical Instrumentation in Root Canal Treatment: A Review of the Literature

Relationship between Large Tubules and Dentin Caries in Human Deciduous Tooth

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