Electrochemical C−H functionalization is a rapidly growing area of interest in organic synthesis. To achieve maximum atom economy, the flow electrolysis process is more sustainable. This allows shorter reaction times, safer working environments, and better selectivities. Using this technology, the problem of overoxidation can be reduced and less emergence of side products or no side products are possible. Flow electro‐reactors provide high surface‐to‐volume ratios and contain electrodes that are closely spaced where the diffusion layers overlap to give the desired product, electrochemical processes can now be managed without the need for a deliberately added supporting electrolyte. Considering the importance of flow electrochemical C−H functionalization, a comprehensive review is presented. Herein, we summarize flow electrolysis for the construction of C−C and C−X (X=O, N, S, and I) bonds formation. Also, benzylic oxidation and access to biologically active molecules are discussed.

Background. Nelumbo nucifera Gaertn. and Nymphaea lotus L. var. pubescens (Willd.) are both aquatic rhizomatous perennial plants mostly found in the tropical region of Nepal, India, Bangladesh, China, and Eastern Asia. Nymphaea pubescens and Nelumbo nucifera plants are famous for their different biological activities such as antidiabetic, antioxidant, hepatoprotective, antidiarrheal, and anti-inflammatory properties. Objective. The present study majorly focused on the determination of in vitro antioxidant and antidiabetic properties of Nelumbo nucifera and Nymphaea pubescens. Methods. In vitro α-glucosidase inhibition was performed using PNPG as a substrate. Antioxidant property of the plant extract was determined by DPPH free radical scavenging assay. The aluminium trichloride method was done for the estimation of total flavonoid content. Likewise, Folin–Ciocalteu reagent was used for determining total phenolic content. Results. The total phenolic content of N. nucifera and N. pubescens was found to be 172.827 ± 0.41 and 194.87 ± 0.93 mg GAE/g, respectively, while the total flavonoid content was reported 17.12 ± 1.04 and 34.59 ± 1.73 mg QE/g, respectively. The IC50 values of the crude extract and its fractions of N. nucifera against the DPPH free radical ranged from 33.46 ± 0.6 to 3.52 ± 0.09 μg/mL, while that of the N. pubescens ranged from 14.30 ± 0.43 to 1.43 ± 0.08 μg/mL. Similarly, for the in vitro α-glucosidase inhibition activity, the IC50 of the crude extract and its fractions of N. nucifera varied from 349.86 ± 2.91 to 29.06 ± 0.24 μg/mL and that of N. pubescens ranged from 224.4 ± 6.85 to 5.29 ± 0.39 μg/mL. Conclusion. Both aquatic plants N. nucifera and N. pubescens show antioxidant properties and can inhibit α-glucosidase in in vitro. Further research is required to identify the inhibiting compounds.

Background: Diabetes has become a considerably more frequent condition and has increased alarmingly in recent years, possibly due to the adoption of modern lifestyle and food habits. The two prominent features of diabetes mellitus are high blood glucose and insulin deficiency, leading to severe consequences. Developing next-generation anti-diabetic medicines with fewer side effects has been a major focus in this situation. Objective: This research aimed to investigate the total phenolic and flavonoid content, antioxidant, antibacterial, α-amylase, and α-glucosidase inhibition activity, as well as in silico analysis of Mimosa pudica L. Methods: The inhibitory activity against α-amylase and α-glucosidase was performed using CNPG3 and PNPG, respectively. Antioxidant activity was estimated using DPPH free radical scavenging assay. The well diffusion method was used for the antibacterial. Using folin- ciocalteu’s reagent, the total phenolic content was determined. The total flavonoid content was determined using the aluminium trichloride method. In addition, molecular docking was performed using autodock vina. Results: Inhibition of α-glucosidase (IC50 = 1.059±0.14μg/mL) was found to be more significant than α-amylase (IC50 = 164.9±0.95μg/mL). The plant was also found to have antioxidant activity (IC50 = 8.207±0.23µg/mL), as well as antibacterial activity against Staphylococcus aureus (ZOI = 13mm) and Bacillus subtilis (ZOI = 10mm). Similarly, the total phenolic and flavonoid content was found to be 177.93±1.8 mg GAE/g, and 19.747±6.11 mg QE/g, respectively. In addition, compounds (stigmasterol, quercetin, and avicularin) isolated from M. pudica showed perfect binding to the enzyme’s active site. Conclusion: Mimosa pudica of Nepalese origin possess potent inhibition against digestive enzymes. Therefore, M. pudica can be used as an alternative therapeutic source to combat the global threat of diabetes.

Background: Rhus chinensis Mill, indigenous wild fruit primarily found in the hilly region of Nepal. The ripe fruit is very sour and considered medicinal as a remedy for colic pain. In addition, their astringent and styptic qualities are used internally to treat illnesses such as diarrhea and hemorrhage. Also, they are used as a common component of polyherbal medications for diabetic mellitus. Objectives: This work aimed to determine the total phenolic and flavonoid content, antioxidant, antibacterial, α-glucosidase, and α-amylase inhibition activity of the crude extract and fractions of Rhus chinensis Mill. Additionally, molecular docking of compounds from Rhus chinensis was performed. Methods: Folin Ciocalteu’s (FC) reagent was used for the estimation of total phenolic content. Likewise, the aluminium trichloride method was applied for the determination of total flavonoid content. For the antioxidant activity, a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay was performed. Furthermore, the substrate-based enzyme inhibition assay was carried out for α-glucosidase and α-amylase inhibition activity of R. chinensis. P-nitrophenyl-α-D-glucopyranoside (PNPG) and 2-Chloro-4-Nitrophenyl-α-D-Maltotrioside (CNPG3) were used as substrates for α-glucosidase and α-amylase inhibition assay, respectively. Similarly, the well-diffusion method was used for the antibacterial activity. Autodock vina was used to perform the molecular docking. Results: The total phenolic and flavonoid content of R. chinensis fruit were found 117.092±1.1 mg GAE/g and 62.41±1.23 mg QE/g, respectively. The IC50 value for antioxidant activity of the crude extract and its fractions ranged from 3.12±1.15μg/mL to 50.85±2.10μg/mL. Similarly, the IC50 for α-glucosidase inhibition ranged from2.33±1.01µg/mL to 28.34±2.79μg/mL. Likewise, The IC50 of R. chinensis crude methanolic extract against α-amylase was 120.3±1.382µg/mL. The antibacterial activity of R. chinensis was effective against gram-positive bacteria; Staphylococcus aureus (ZOI=11.0) and Bacillus subtilis (ZOI=9.0). Quercetin-3-O-rhamnoside and Myricetin 3-O-rhamnoside showed excellent binding to the active site of protein with binding energy -9.4kcal/mol and -9.6kcal/mol, respectively. Conclusion: Rhus chinensis Mill is a potent antioxidant and inhibits enzymes; α-glucosidase and α-amylase. In addition, the methanolic extract of this plant shows antibacterial activity. However, further research is required to determine the inhibiting compounds.

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Background: The use of medicinal plants as supplemental or alternative medicine is widespread around the world. For the development of new drugs, studies on these medicinal plants that include pharmacological and toxicological assessments are crucial. Objective: This work aimed to find the total phenolic and flavonoid content, antioxidant, antibacterial, and antidiabetic potential of the traditionally used medicinal plant Mimosa rubicaulis Lam. Methods: The in vitro antidiabetic potential of methanolic extract and its fractions of the roots of M. rubicaulis were performed via enzyme (α-glucosidase and α-amylase) inhibition assays. Antioxidant and anti-inflammatory activities were carried out using 2,2 Diphenyl-1-picrylhydrazyl (DPPH), and reactive oxygen species (ROS) inhibiting methods. Well diffusion method is applied for antibacterial activity. Autodock vina was used for molecular docking. Results: The crude extract reported the highest inhibition activity against α-glucosidase with an IC50 value of 10.29 ± 0.35 μg/mL compared to the standard acarbose IC50 value of 5.653 ± 0.29 µg/mL. Similarly, the ethyl acetate (EA) fraction disclosed significant inhibition against α-amylase with an IC50 value of 108.7 ± 0.66 μg/mL compared to the standard drug’s IC50 value of 6.01 ± 0.14 µg/mL. Likewise, the EA fraction showed the maximum antioxidant activity with an IC50 value of 11.89 ± 1.05μg/mL among the crude extract and its fractions. Conclusion: Mimosa rubicaulis was found to have α-glucosidase and α-amylase inhibition, anti-inflammatory, and antibacterial activity. To the best of our knowledge, this is the first report of α-glucosidase and α-amylase inhibition activity of this plant. Further studies on this plant are required to isolate potent compounds.