α-Glucosidase and α-Amylase Inhibition Study andIn SilicoAnalysis ofMimosa pudicaL. of Nepalese Origin

Abstract

Background: Diabetes has become a considerably more frequent condition and has increased alarmingly in recent years, possibly due to the adoption of modern lifestyle and food habits. The two prominent features of diabetes mellitus are high blood glucose and insulin deficiency, leading to severe consequences. Developing next-generation anti-diabetic medicines with fewer side effects has been a major focus in this situation. Objective: This research aimed to investigate the total phenolic and flavonoid content, antioxidant, antibacterial, α-amylase, and α-glucosidase inhibition activity, as well as in silico analysis of Mimosa pudica L. Methods: The inhibitory activity against α-amylase and α-glucosidase was performed using CNPG3 and PNPG, respectively. Antioxidant activity was estimated using DPPH free radical scavenging assay. The well diffusion method was used for the antibacterial. Using folin- ciocalteu’s reagent, the total phenolic content was determined. The total flavonoid content was determined using the aluminium trichloride method. In addition, molecular docking was performed using autodock vina. Results: Inhibition of α-glucosidase (IC50 = 1.059±0.14μg/mL) was found to be more significant than α-amylase (IC50 = 164.9±0.95μg/mL). The plant was also found to have antioxidant activity (IC50 = 8.207±0.23µg/mL), as well as antibacterial activity against Staphylococcus aureus (ZOI = 13mm) and Bacillus subtilis (ZOI = 10mm). Similarly, the total phenolic and flavonoid content was found to be 177.93±1.8 mg GAE/g, and 19.747±6.11 mg QE/g, respectively. In addition, compounds (stigmasterol, quercetin, and avicularin) isolated from M. pudica showed perfect binding to the enzyme’s active site. Conclusion: Mimosa pudica of Nepalese origin possess potent inhibition against digestive enzymes. Therefore, M. pudica can be used as an alternative therapeutic source to combat the global threat of diabetes.

In Vitro Antioxidant and Antimicrobial Potency of Mimosa pudica of Nepalese Terai Region: Insight into L-Mimosine as an Antibacterial Agent

“…In the study conducted by (Shrestha et al) on the same plant extract, the antibacterial activity against Staphylococcus aureus and Bacillus subtilis was noted. e zone of inhibition (ZOI) given by Mimosa extract against these bacteria was found to be 13 mm and 10 mm, respectively [27].…”

“…Flavonoids are secondary metabolites possessing antioxidant properties, the endurance of which is determined by the number and position of free OH groups. e reports of past studies on Mimosa pudica show that Shrestha et al reported a TFC of 19.747 ± 6.11 mg QE/g and Srishti Prashar et al determined a TFC of 49.47 mg QE/g in methanolic extract of M. pudica [27,28].…”

Antioxidant, α-Glucosidase, and α-Amylase Inhibition Activities of Erythropalum scandens Blume

“…Type 2 diabetes mellitus (T2DM) is the most prevalent type of diabetes, characterized by hyperglycemia, insulin resistance, and relative insulin defciency [1,2]. One of the best methods to manage postprandial hyperglycemia is enzyme (α-glucosidase and α-amylase) inhibition [3].…”

α‑Glucosidase and α‑amylase Inhibitory assay of Fractions and Crude Extract of Zingiber officinale (Ginger) and Evaluation of Its Antioxidant (ABTS and DPPH) Activity

“…It can be said that this is due to the fact that the rhizome actually possesses useful and important characteristics and qualities such as strong flavour, quality and distinctive nutritional value. The rhizomes contain two distinct categories of substances: volatile compounds, which are responsible for the production of the essential oil, and non-volatile compounds, which include oleoresin, which is a source of pungent, as well as other phytochemicals that have biological activities that are beneficial to human health, such as flavonoids and phenolics [8]. In essence, the essential oil is composed of the volatile components.…”