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Human T-cell leukemia virus type I (HTLV-I) provirus DNA from the cultured cell line HUT 102 and from peripheral mononuclear cells (PBMC) of anti-HTLV-I antibody-positive Japanese blood donors was detected by the nested double polymerase chain reaction (PCR) method. This procedure consists of a first amplification and a second amplification with the products of the first amplification and primers interior to the first primers. Using this method, we demonstrated that it is possible to detect single-template DNA. Polyacrylamide gel electrophoresis of the nested double PCR products, with our primers, revealed three bands with excess amounts of template DNA, two bands with moderate amounts, and a single band with limited amounts. The amount of provirus in PBMC was roughly estimated from the results of the nested double PCR. Particle agglutination (PA) assays and indirect immunofluorescence testing (IF) with mixed MT-2 cells and Molt-4 cells as targets to detect anti-HTLV-I antibody were performed, and the results were compared with those of the nested double PCR of the pX region. None of the 101 PA-negative samples were positive in either the IF or PCR test. Of the 155 samples that were antibody positive by the PA assay, 57 were positive by both PCR and IF. Furthermore, the results of the IF and PCR tests coincided completely. It was therefore concluded that the IF method is most appropriate for confirmation of the PA assay currently used in most diagnostic laboratories and blood centers.

The present study examined the effects of voice and physical appearance on interpersonal attraction. Furthermore, the attributes of voice that enhance interpersonal attraction were investigated. In the first study the subjects were 25 female students from one university and the target persons were four male students from another university. The subjects rated attractiveness of voice and physical appearance, and the overall interpersonal attraction of the target persons. The attractiveness of voice and physical appearance had independent effects on interpersonal attraction. In the second study the subjects were 62 students (20 males, 42 females) from one university and the target persons were 16 students from another university (eight of each sex). The results of the first study were replicated. Bright, generous voices, low vocal pitch and a small range of vocal pitch increased interpersonal attraction.

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This study aims to examine memorable tourism experiences and their role in subsequent well-being. To achieve this, we carried out longitudinal surveys during the winter vacation period, recruiting participants via an online marketing research company. A total of 230 participants completed questionnaires comprising questions seeking demographic information and psychological scales at four time points so that the changes in their well-being could be assessed. Our findings indicate that having a memorable experience during a vacation had positive influences not only on the recovery experience at that time, but also on the recovery experience during subsequent daily life. Although the recovery experience during and after the vacation was profoundly involved, it should be noted that only the recovery experience after returning to everyday life was further associated with individuals’ well-being. The results demonstrate new findings on how extraordinary experiences during vacations influence commonplace activities.

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