The expression of matrix metalloproteinases (MMPs) has been implicated in the invasion and metastasis of cancer cells. Here we examined the effect of ascochlorin, a prenyl-phenol anti-tumor compound from the fungus Ascochyta viciae, on the regulation of signaling pathways that control MMP-9 expression in human renal carcinoma (Caki-1) cells. Ascochlorin reduced the invasive activity of Caki-1 cells and inhibited phorbol 12-myristate 13-acetate-induced increases in MMP-9 expression and activity in a dose-dependent manner. Reporter gene, electrophoretic mobility shift, kinase inhibitor assays, and in vitro kinase assay showed that ascochlorin inhibits MMP-9 gene expression by suppressing activation of the nuclear transcription factor activator protein-1 (AP-1) via the extracellular signalregulated kinase 1 and 2 pathway. The AP-1 family member most specifically affected by ascochlorin was Fra-1. Ascochlorin did not affect the activation of the c-Jun N-terminal or p38 kinase pathways. Moreover, transfection of Caki-1 cells with AP-1 decoy oligodeoxynucleotides resulted in the suppression of phorbol 12-myristate 13-acetate-induced MMP-9 expression and invasion. In conclusion, ascochlorin represents a unique natural anti-tumor compound that specifically inhibits MMP-9 activity through suppression of AP-1-dependent induction of MMP-9 gene expression.Ascochlorin (see Fig. 1A), a prenyl-phenol compound isolated from the fungus, Ascochyta viciae, was originally found to have antiviral antibiotic activity (1). In addition to its antiviral and anti-fungal activity, natural and synthetic derivatives of ascochlorin reduce serum cholesterol and triglyceride levels suppress hypertension and tumor development and ameliorate type I and II diabetes (2-9). Moreover, several ascochlorin derivatives have been reported to be potent agonists of nuclear hormone receptors, including peroxisome proliferator-activated receptor ␥, suggesting that the structure of ascochlorin would be useful in designing modulators of nuclear receptors (6, 10, 11, 13).The most well known extracellular matrix (ECM) 1 -degrading enzymes are the matrix metalloproteinases (MMPs). MMPs are a family of zinc-dependent endoproteinases that are capable of degrading all the components of the ECM. MMPs are structurally and functionally homologous proteins, with at least 20 known members. MMPs can be divided into four families based on structure and substrate specificity: collagenases, gelatinases, stromelysins, and membrane-associated MMPs. Among the previously reported human MMPs, gelatinase-A (MMP-2) and gelatinase-B (MMP-9) are key enzymes that degrade type IV collagen (14, 15). These two gelatinases share structural and catalytic similarities, but transcription of the MMP-2 and MMP-9 genes is independently regulated due to distinct arrays of regulatory elements in the gene promoters. Regulated expression of MMP-9 has been implicated in renal development, macrophage differentiation, atherosclerosis, inflammation, rheumatoid arthritis, and tumor invasion (16 -17). I...