Detection of human T-cell leukemia virus type I (HTLV-I) provirus in an infected cell line and in peripheral mononuclear cells of blood donors by the nested double polymerase chain reaction method: comparison with HTLV-I antibody tests

Matsumoto, Chieko; Mitsunaga, Shigeki; Oguchi, Takashi; Mitomi, Yoshitada; Shimada, Tooru; Ichikawa, Akiko; Watanabe, Junnosuke; Nishioka, Kusuya

doi:10.1128/jvi.64.11.5290-5294.1990

Cited by 59 publications

(18 citation statements)

References 29 publications

(20 reference statements)

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“…Indeed, Saito et al (1989Saito et al ( , 1990 reported several cases of HTLV-I-seronegative and PCR-positive children who were born to HTLV-I carrier mothers. On the other hand, Matsumoto et al (1990) reported a correlation between PCR positivity and IF seropositivity in HTLV-I-infected donors. In the present study, we used the IF method for the serological test and investigated the level of HTLV-I infection among the children.…”

Section: Discussionmentioning

confidence: 98%

Inhibitory effect of maternal antibody on mother‐to‐child transmission of human T‐lymphotropic virus type I

Takahashi

Takezaki

Oki

et al. 1991

Intl Journal of Cancer

133

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In order to evaluate the protective role of the maternal antibody against mother-to-child transmission of HTLV-I, we followed a total of 780 children born to HTLV-I carrier mothers by investigating the level of anti-HTLV-I antibody transferred in utero, decline of the maternal antibody and seroconversion in post-natal life. The anti-HTLV-I antibody was positively detected within the first 3-6 months of life and declined at 6-12 months after birth in all children. After the maternal antibody declined, seroconversion occurred in some of the children following either breast feeding or bottle feeding. The seroconversion rates of short-term (less than or equal to 6 months) and long-term (greater than or equal to 7 months) breast feeders were 4.4% (4/90 cases) and 14.4% (20/139 cases), and the rate of bottle feeders was 5.7% (9/158 cases). Long-term breast feeding yielded more seroconverters than short-term breast feeding; 14.4% (20/139 cases) vs. 4.4% (4/90 cases), RR = 3.68, p = 0.018. The seroconversion rate of short-term breast feeders was nearly equal to that of bottle feeders; 4.4% (4/90 cases) vs. 5.7% (9/158 cases), RR = 0.770, p = 0.471. When neonatal lymphocytes were cultured with breast milk cells of HTLV-I carrier mothers, the in vitro infection of HTLV-I was inhibited by the addition of HTLV-I-seropositive cord-blood plasma. Our results suggest that the maternal antibody may inhibit HTLV-I infection by short-term breast feeding but not by long-term breast feeding after decline of the maternal antibody.

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Section: Discussionmentioning

confidence: 98%

Inhibitory effect of maternal antibody on mother‐to‐child transmission of human T‐lymphotropic virus type I

Takahashi

Takezaki

Oki

et al. 1991

Intl Journal of Cancer

133

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“…The PCR method has been used in both qualitative and quantitative measurement of proviral DNA (Ehrlich et al, 1990). Matsumoto et al (1990) used a nested doublc PCR method to examine PBMC from HTLV-I-seropositive blooddonor samples and found that 48 of 57 carriers had a high level with more than 200 copies in 1.5 x lo5 PBMC.…”

Section: Discussionmentioning

confidence: 99%

“…An alternative method is the polymerase chain reaction (PCR) which increases target DNA sequences to a detectable level (Saiki et al, 1985). The detection of HTLV-I proviral DNA in peripheral blood mononuclear cells (PBMC) of carriers by this method has been reported (Matsumoto et al, 1990;Nakayama et al, 1990;Ehrlich et al, 1990).…”

mentioning

confidence: 99%

High HTLV‐I proviral DNA level associated with abnormal lymphocytes in peripheral blood from asymptomatic carriers

Tachibana¹,

Okayama²,

Ishihara³

et al. 1992

Intl Journal of Cancer

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The level of proviral DNA in peripheral blood mononuclear cells from a representative group of asymptomatic HTLV-I carriers in Miyazaki district, an HTLV-I endemic area in Japan, was determined by a single-cycle polymerase chain reaction method (PCR). Of 217 subjects, 26% had a high level of proviral DNA, 43% a medium level, 18% a low level, and 13% an undetectable level. In the high-DNA group, 60% had at least 0.6% abnormal lymphocytes on peripheral blood smears, significantly higher than in those with low DNA levels (19%). This association was present for men of all ages and for women under 55. Men were more than twice as likely to have abnormal lymphocytes as well as high levels of proviral DNA. These differences may reflect different host responses to the virus by sex or by the time or route of infection. This study supports the utility of PCR for molecular screening in epidemiologic studies of the natural history of HTLV-I, and may lead to the identification of those carriers who are at greatest risk of developing HTLV-I-induced malignancy.

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“…Screening-positive blood donations underwent an indirect immunofluorescence (IF)-based confirmatory test for the presence of anti-HTLV-1 antibodies, as described previously. 15 In the case that more than two testers were undecided about an IF result, based on weak positive and negative references, the sample was classified as IF indeterminate.…”

Section: Routine Screening and Confirmatory Tests For Anti-htlv-1 Amentioning

confidence: 99%

Analysis of HTLV‐1 proviral load (PVL) and antibody detected with various kinds of tests in Japanese blood donors to understand the relationship between PVL and antibody level and to gain insights toward better antibody testing

Matsumoto

Sagara²,

Sobata

et al. 2017

Journal of Medical Virology

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Adult T-cell leukemia/lymphoma (ATL) occurs in approximately 5% of individuals infected with human T-cell leukemia virus type 1 (HTLV-1). A high proviral load (PVL; more than four copies per 100 peripheral blood mononuclear cells (PBMCs) or 1.6 copies per 100 blood leukocytes) and being male are risk factors for ATL development. Whether anti-HTLV-1 antibody level is related to such risk is unknown. Here, PVL and antibody levels were examined using real-time PCR and other tests in 600 HTLV-1 positive screened Japanese blood donors to understand the relationship between PVL and antibody level in asymptomatic carriers and to gain insights toward better antibody testing for HTLV-1 infection. The 430 donors in whom proviral DNA was detected were considered as true positives for HTLV-1 infection. Among donors aged 40 years or older, more males than females had a PVL corresponding to more than 1.6% infected leukocytes, and an antibody titer below the median (P = 0.0018). In antibody tests using an HTLV-1 positive cell line or Env antigens there was a large discrepancy in antibody titer among 13 provirus-positive samples, probably suggesting that antibody-based screening tests should incorporate multiple HTLV-1 antigens, such as Gag and Env antigens.

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Detection of human T-cell leukemia virus type I (HTLV-I) provirus in an infected cell line and in peripheral mononuclear cells of blood donors by the nested double polymerase chain reaction method: comparison with HTLV-I antibody tests

Cited by 59 publications

References 29 publications

Inhibitory effect of maternal antibody on mother‐to‐child transmission of human T‐lymphotropic virus type I

Inhibitory effect of maternal antibody on mother‐to‐child transmission of human T‐lymphotropic virus type I

High HTLV‐I proviral DNA level associated with abnormal lymphocytes in peripheral blood from asymptomatic carriers

Analysis of HTLV‐1 proviral load (PVL) and antibody detected with various kinds of tests in Japanese blood donors to understand the relationship between PVL and antibody level and to gain insights toward better antibody testing

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