Background As one typical cardiovascular disease, atherosclerosis severely endanger people’ life and cause burden to people health and mentality. It has been extensively accepted that oxidative stress and inflammation closely correlate with the evolution of atherosclerotic plaques, and they directly participate in all stages of atherosclerosis. Regarding this, anti-oxidation or anti-inflammation drugs were developed to enable anti-oxidative therapy and anti-inflammation therapy against atherosclerosis. However, current drugs failed to meet clinical demands. Methods Nanomedicine and nanotechnology hold great potential in addressing the issue. In this report, we engineered a simvastatin (Sim)-loaded theranostic agent based on porous manganese-substituted prussian blue (PMPB) analogues. The biomimetic PMPB carrier could scavenge ROS and mitigate inflammation in vitro and in vivo. Especially after combining with Sim, the composite Sim@PMPB NC was expected to regulate the processes of atherosclerosis. As well, Mn2+ release from PMPB was expected to enhance MRI. Results The composite Sim@PMPB NC performed the best in regulating the hallmarks of atherosclerosis with above twofold decreases, typically such as oxidative stress, macrophage infiltration, plaque density, LDL internalization, fibrous cap thickness and foam cell birth, etc. Moreover, H2O2-induced Mn2+ release from PMPB NC in atherosclerotic inflammation could enhance MRI for visualizing plaques. Moreover, Sim@PMPB exhibited high biocompatibility according to references and experimental results. Conclusions The biomimetic Sim@PMPB theranostic agent successfully stabilized atherosclerotic plaques and alleviated atherosclerosis, and also localized and magnified atherosclerosis, which enabled the monitoring of H2O2-associated atherosclerosis evolution after treatment. As well, Sim@PMPB was biocompatible, thus holding great potential in clinical translation for treating atherosclerosis. Graphic abstract

In this study, ultraviolet‐B radiation (UV‐B) was used to effect the accumulation of vitamin C, phenolics and flavonoids in mung bean sprouts. Results indicate that the content of vitamin C and flavonoids increased during the initial period, and after a brief decline, reached peak levels of 25.29 ± 1.02 mg/100 g FW and 726.67 ± 7.35 mg/100 g DW, respectively, at 2.5 h (1.845 kJ m−2), while the peak levels of the phenolics were 10741.33 ± 68.04 mg/100 g DW. The thiobarbituric acid reactive substances (TBARS) content decreases with the increase in irradiation time. The activities of the related enzymes, including phenylalanine ammonia‐lyase (PAL), L‐galactono‐1, 4‐lactone dehydrogenase (GalLDH), peroxidase (POD), polyphenol oxidase (PPO) and chalcone isomerase (CHI) were determined, which showed strong correlations with the change in the content of vitamin C, phenolics and flavonoids. In conclusion, the accumulation of vitamin C, phenolics and flavonoids in mung bean sprouts can be promoted by a low‐dose UV‐B irradiation.

Grouper iridovirus (GIV) is one of the most serious pathogens in mariculture and causes high mortality rates in cultured groupers; then, effective medicines for controlling GIV infections are urgently needed. Viola philippica is a well‐known medicinal plant, and the application of V. philippica aqueous extracts against GIV infection was assessed by different methods in this study. The results showed that the working concentration of V. philippica aqueous extracts was 10 mg/ml. V. philippica aqueous extracts below 10 mg/ml have no significant cytotoxic effects on cell viability, while extracts over 15 mg/ml decreased cell viability and showed cytotoxic activity. V. philippica aqueous extracts had excellent inhibitory effects against GIV infection in vitro and in vivo. The possible antiviral mechanism of V. philippica was further analysed, which indicated that V. philippica did no damages to GIV particles, but it could disturb GIV binding, entry and replication in host cells. V. philippica had the best inhibitory effects against GIV during viral infection stage of binding and replication in host cells. Overall, the results suggest that appropriate concentration of V. philippica aqueous extracts has great antiviral effects, making it an interesting candidate for developing effective medicines for preventing and controlling GIV infection in farmed groupers.

Summary A comparative assessment of the phytochemical profiles and antioxidant activities of seven cultivars of Aloe was conducted to evaluate the potential health benefits of Aloe. Aloe arborescens contained the highest levels of phenolic content, total antioxidant capacity by the oxygen radical scavenging capacity assay and cellular antioxidant activity assay. Aloe vera showed the highest levels of flavonoid content and antioxidant capacity by the peroxyl radical scavenging capacity assay. Aloe greenii had the highest CAA value with a PBS wash before adding ABAP. There were no significant differences observed between Aloe arborescens and Aloe greenii. Aloin, aloe‐emodin‐8‐O‐beta‐D‐glucopyranoside, catechin, epicatechin, sinapic acid and chlorogenic acid were identified in Aloe samples by the HPLC analysis. Aloin, aloe‐emodin‐8‐O‐beta‐D‐glucopyranoside and catechin showed strong relationships with antioxidant activity. Significant levels of aloin, aloe‐emodin‐8‐O‐beta‐D‐glucopyranoside and catechin were determined in Aloe greenii, Aloe vera and Aloe saponaria, respectively.

: High-level reactive oxygen species (ROS) have been reported to exert a robust anti-tumor effect by inducing cell apoptosis or necroptosis. Based on the Fenton reaction or Fenton-like reaction, a therapeutic strategy (i.e., chemodynamic therapy (CDT)) is proposed, where hydroxyl radicals (•OH) that are one typical ROS via the spontaneous activation by endogenous stimulus can be produced to kill tumors. Moreover, high-level ROS can also facilitate tumor-associated antigen exposure, which benefits phagocytosis of corpses and debris by antigen-presenting cells (e.g., dendritic cells (DCs)) and further activates systematic immune responses. Great efforts wherein nanotechnology is underlined have been made in interdisciplinary communities to witness the development of this field. To provide a comprehensive understanding of CDT, the state of art of strategies on nanotechnology-enabled CDT is discussed in detail. In particular, the combination of CDT and its augmented immunotherapy against tumor for overcoming the poor outcome that mono-CDT suffers from is highlighted. Moreover, the potential challenges will also be discussed.

Achyranthes bidentata is a popular perennial medicine herb used for 1000s of years in China to treat various diseases. Although this herb has multiple pharmaceutical purposes in China, no transcriptomic information has been reported for this species. In addition, the understanding of several key pathways and enzymes involved in the biosynthesis of oleanolic acid and ecdysterone, two pharmacologically active classes of metabolites and major chemical constituents of A. bidentata root extracts, is limited. The aim of the present study was to characterize the transcriptome profile of the roots and leaves of A. bidentata to uncover the biosynthetic and transport mechanisms of the active components. In this study, we identified 100,987 transcripts, with an average length of 1146.8 base pairs. A total of 31,634 (31.33%) unigenes were annotated, and 12,762 unigenes were mapped to 303 pathways according to the Kyoto Encyclopedia of Genes and Genomes pathway database. Moreover, we identified a total of 260 oleanolic acid and ecdysterone genes encoding biosynthetic enzymes. Furthermore, the key enzymes involved in the oleanolic acid and ecdysterone synthesis pathways were analyzed using quantitative real-time polymerase chain reaction, revealing that the roots expressed these enzymes to a greater extent than the leaves. In addition, we identified 85 ATP-binding cassette transporters, some of which might be involved in the translocation of secondary metabolites.

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Autophagy as a double-edged sword features an oncolytic impediment/promotion balance, which manipulates tumor progression. From this perspective, a sonosensitizer-free targeting oncolytic nanoplatform (SFTON) consisting of chloroquine (CQ) and porphyrin-structured metal centers (PMCS) was engineered to break this balance for enhancing antitumor activity. Porphyrin structure retention in a ZIF-8-derived hydrophobic carbon skeleton retained high stability and high sonocatalytic activity, and the hydrophobic carbon skeleton capable of adsorbing air provided cavitation nuclei for further elevating sonocatalytic activity. More significantly, the encapsulated CQ as the autophagy inhibitor reprogrammed autophagy, terminated the autophagy-induced self-protection or self-detoxification, and unfroze the resistances to reactive oxygen species (ROS) therapy associated with ROS accumulation and ROS activity. Systematic experiments reveal the action principles and validate that the induced apoptosis and blockaded autophagosome escalation into the autolysosome were two activated pathways to magnify the antitumor sonocatalytic therapy. Contributed by these actions, the SFTON-unlocked oncolytic impediment/promotion balance disruption strategy acquired considerable antitumor outcomes in vivo and in vitro against liver tumor progression, especially after combining with AS1411-mediated active targeting. This impediment/promotion balance disruption enabled by the SFTON can serve as a general method to elevate ROS-based antitumor activity.