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The study of the method in the determination of the isoleucine/leucine ratio in-the leucine fraction from the hydrolysates of casein, insulin and edestin gave results which agree with those obtained by microbiological methods except in the case of edestin where there is a very large discrepancy' We are grateful to. Prof. A. C. Chibnall, at whose instigatien this investigation was undertaken, for his interest and helpfil advice. We are indebted to the Medical Research Council (S.E.D.) and to the Agricultural Research Council (G.R.T.) for grants during the tenures of which this investigation was undertaken.

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