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In this study were to explore the properties of interaction between cellulose and calcium carbonate particle (CaCO3) which derive from Papia Undulates Shell in procedure of biocomposite synthesis. The structural properties of cellulose powder Papia Undulates Shell and cellulose-calcium carbonate composite film were investigated by using X-ray Diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) and the compositions of cellulose/CaCO3 biocomposite film were studied by Energy Dispersive X-ray Fluorescence (EDXRF). The experimental results demonstrated the morphology of Papia Undulates Shell were crystalline aragonite phase and the cellulose have structural as amorphous-crystalline but after film forming the composite film between cellulose and Papia Undulates Shell also have amorphous structural. The result of FTIR used to confirmed the formation of bonding between molecular, it indicated that the cellulose/CacO3 biocomposite film had good biocompatibility due to the biocomposite film have both characteristic feature of CO3-2 group (~874 cm-1 and ~713 cm-1) and the glucose of cellulose at ~1635, ~1064 and ~946 cm-1. Another that, the result from EDXRF shown the chemical composition of organic compound of cellulose/CaCO3 biocomposite film was highest with 99.437 while the Papia Undulates Shell have 0.341 Wt% with corresponding with the ratio of filler material which mixture as 1%. So that, the cellulose/calcium carbonate bicomposite film could be candidate for biocomposite film application.