The present paper describes the development of a reverse phase chromatographic (RPLC) method for montelukast sodium in the presence of its impurities and degradation products generated from forced degradation studies. The drug substance was subjected to stress conditions of hydrolysis, oxidation, photolysis and thermal degradation. The degradation of montelukast sodium was observed under acid and oxidative environment. The drug was found to be stable in other stress conditions studied. Successful separation of the drug from the process impurities and degradation products formed under stress conditions were achieved on an Atlantis dC18 (250 x 4.6 mm) 5 µm column. The gradient LC method employs solution A and solution B as mobile phase. The solution A contains aqueous 0.1% OPA and solution B contains a mixture of water, acetonitrile (5:95 v/v). The HPLC method was developed and validated with respect to linearity, accuracy, precision, specificity and ruggedness.
Since the existence of life, all species are dependent on nature for food, shelter and other requirements. From the inception of civilizations, human beings are also dependent on nature to fulfil their requirements of food, shelter and health with the help of natural sources. Natural compounds have been recognized to perform various biological functions such as; defence against pathogens and medicinal activity etc. The plants are considered to be nature's largest laboratory that synthesizes the myriad of novel compounds and may be considered as inexhaustible source of bioactive compounds. These compounds possess chemo-diversity and broadly categorized as alkaloids, terpenoids, phenols, tannins, saponins and glycosides etc. which are reported to exhibit various biological activities. In this study the hexane extract of Cordia obliqua was prepared and analyzed by FTIR and GCMS to analyze the presence of phytochemicals. GCMS analysis revealed that this extract contains large number of phytochemicals such as 3-Hexen-2-one, n-
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.