A Validated RP‐HPLC Method for theDetermination of Impurities in Montelukast Sodium

Rashmitha, N.; Raj, T. Joseph Sunder; Srinivas, C. V.; Srinivas, N.; Ray, Uttam Kumar; Sharma, Hemant; Mukkanti, K.

doi:10.1155/2010/156593

Cited by 22 publications

(15 citation statements)

References 1 publication

(1 reference statement)

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“…Method which has been previously validated in HPLC device is as follows: TSP HPLC device was used [18]. Mobile phase was water-acetonitrile (5:95 vv) of 3 mL/min rate was used.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model

Lafçı

Gedik

Korkmaz

et al. 2013

J Cardiothorac Surg

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BackgroundThe thoracic or thoracoabdominal aortic aneurysm surgery may cause spinal cord ischemia because of aortic cross-clamping and may result in severe postoperative complications caused by spinal cord injury. Ischemia/reperfusion injury may directly or indirectly be responsible for these complications. In this study we sought to determine whether combination of iloprost and montelukast can reduce the ischemia/reperfusion injury of spinal cord in a rat model.MethodsMedulla spinalis tissue concentrations of interleukin-6 (IL-6), myeloperoxidase (MPO) and heat shock protein 70 (HSP-70) were determined in 3 groups of Spraque Dawley rats: control group (operation with cross clamping and intraperitoneal administration of 0.9% saline, n = 7), sham group (operation without cross clamping, n = 7), and study group (operation with cross-clamping and intraperitoneal administration of iloprost (25 ng/kg) and montelukast (1 mg/kg), n = 7). The abdominal aorta was clamped for 45 minutes, with a proximal (just below the left renal artery) and a distal (just above the aortic bifurcation) clip in control and study groups. Hindlimb motor functions were evaluated at 6, 12, 24, and 48 hours using the Motor Deficit Index score. All rats were sacrificed 48 hours after the procedure and spinal cord tissue levels of myeloperoxidase, interleukin-6, and heat shock protein (HSP-70) were evaluated as markers of oxidative stress and inflammation. Histopathological analyses of spinal cord were also performed.ResultsThe tissue level of HSP-70 was found to be similar among the 3 groups, however, MPO was highest and IL-6 receptor level was lowest in the control group (p = 0.007 and p = 0.005; respectively). In histopathological examination, there was no significant difference among the groups with respect to the neuronal cell degeneration, edema, or inflammation, but vascular congestion was found to be significantly more prominent in the control group than in the sham or in the study group (p = 0.05). Motor deficit index scores at 24 and 48 hours after ischemia were significantly lower in the study group than in the control group.ConclusionThis study suggests that combined use of iloprost and montelukast may reduce ischemic damage in transient spinal cord ischemia and may provide better neurological outcome.

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“…Method which has been previously validated in HPLC device is as follows: TSP HPLC device was used [18]. Mobile phase was water-acetonitrile (5:95 vv) of 3 mL/min rate was used.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model

Lafçı

Gedik

Korkmaz

et al. 2013

J Cardiothorac Surg

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“…reviewed. 4 Various techniques such as uv-spectrophotometry, [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] potentiometry, voltammetry, [27][28] high-performance liquid chromatography, [29][30][31][32][33][34][35][36][37][38][39] ultra-performance liquid chromatography, 40 highperformance thin layer chromatography [41][42][43][44][45][46] and capillary electrophoresis 47 have been reported for the determination of PGH in bulk sampleand tablets.…”

Section: Pioglitazone Hydrochloride (Pgh) Chemically Known As 5-[[4-mentioning

confidence: 99%

Spectrophotometric assay of pioglitazone hydrochloride using permanganate in acidic and basic media

Basavaiah¹,

Rajendraprasad²

2018

10.5267/j.ccl

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Pioglitazone hydrochloride (PGH) is an oral anti-hyperglycemic agent used in the treatment of type-2 diabetes mellitus. Potassium permanganate was found to oxidize PGH both in acidic and basic conditions, based on which two simple and sensitive methods were developed for its determination in bulk sample and tablets, and validated. In the first method (indirect method), PGH was reacted with a measured excess of standard permanganate in H2SO4 medium, and the residual oxidant was determined by measuring its absorbance at 550 nm. The second method (Direct method) entails treating PGH with permanganate in NaOH medium, followed by the measurement of the resulting bluish-green manganite at 610 nm. Experimental variables affecting the reactions were studied and optimized. Under optimum conditions, linear relationships with good correlation coefficients were found between absorbance and concentration in the ranges, 1.25 -25 µg mL -1 (Indirect method) and 1-12 µg mL -1 (Direct method) with respective molar absorptivity values of 1.10 × 10 4 and 2.77 × 10 4 l mol -1 cm -1 . The limits of detection (LOD) and quantification (LOQ) were 0.36 and 1.08 (Indirect method) and 0.23 and 0.69 µg mL -1 (Direct method). Intra-day and inter-day precisions were satisfactory, with %RSD values of ≤2.11, and the respective accuracies were excellent with %RE values of ≤2. The methods were also validated for robustness, ruggedness and selectivity. The methods were applied to the determination of PGH in its tablets with good accuracy and precision, and no interference from the tablet additives was encountered. The results were also compared with those obtained by a reference method.

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“…100 μL of this solution was injected into the semi-preparative high-performance liquid chromatography (HPLC) system and fractioned according to time of extracting from chromatogram. We use a method in the HPLC device which was previously validated [16].…”

Section: Study Groupsmentioning

confidence: 99%

Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi

Budak¹,

Korkmaz²,

Gedik³

et al. 2017

TURKISH JOURNAL of CLINICS and LABORATORY

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Aim: The thoracoabdominal aortic aneurysm surgery may cause splanchnic ischemia and may result in severe postoperative complications caused by liver and/or kidney injury. This study investigated the effect of temporary occlusion of the aorta on the development of I/R injury of liver and kidney and the protective effect of montelukast, a selective reverse CysLT1 receptor antagosit. Material and Methods: Twenty-one male Sprague-Dawley rats were randomly assigned to three groups (n=7 per group) as G1 (no aortic occlusion and montelukast administration), G2 (45 min. aortic occlusion; no montelukast administration) and G3 (45 min. aortic occlusion, 10 mg/kg montelukast administration). At the 48th hour of reperfusion, kidney and liver samples were dissected for histopathological evaluation and immunohistochemical staining for HSP70, interleukin-6 and myeloperoxidase (MPO). Results: Tissue samples taken from the kidney showed significant intergroup differences in terms of tubular cell degeneration, cytoplasmic vacuole formation, hemorrhage and tubular dilatation (P = 0.001, P = 0.017, P = 0.001, P = 0.001 respectively). There was a significant difference with regards to HSP-70 stain (P = 0.01) in favor of G1 and G3 with respect to G2. Hepatocyte degeneration in the liver samples of G3 was significantly lower than that of G1 and G2 (P = 0.027). Conclusion: The findings of the current study demonstrated that montelukast has protective effects on both kidney and liver; and were confirmed histopathologically and immunohistochemically. The protective effects of montelukast can be attributed to its potential of anti-oxidative and anti-inflammatory actions.

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A Validated RP‐HPLC Method for theDetermination of Impurities in Montelukast Sodium

Cited by 22 publications

References 1 publication

Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model

Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model

Spectrophotometric assay of pioglitazone hydrochloride using permanganate in acidic and basic media

Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi

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