No abstract

We used 69 steers of varying percentage Brahman (B) breeding (0% B, n = 11; 25% B, n = 13; 37% B, n = 10; 50% B, n = 12; 75% B, n = 12; 100% B, n = 11) to study the relationship between carcass traits, the calpain proteinase system, and aged meat tenderness in intermediate B crosses. Calpains and calpastatin activities were determined on fresh longissimus muscle samples using anion-exchange chromatography. The USDA yield and quality grade data (24 h) were collected for each carcass. Longissimus steaks were removed and aged for 5 or 14 d for determination of shear force and 5 d for sensory panel evaluation. Even though some yield grade factors were affected by the percentage of B breeding, USDA yield grades did not differ (P > .15) between breed types. Marbling score and USDA quality grade decreased linearly (P < .01) with increasing percentage of B breeding. Shear force after 5 and 14 d of aging was higher (P < .05) in the 100% B steers than in all other breed types, which were not significantly different. Sensory panel tenderness and connective tissue scores decreased linearly (P < .05) with increasing B breeding. A quadratic effect was also noted for tenderness and connective tissue scores; 37% B steers received the highest scores. A similar response was found in mu-calpain activities; the 37% B steers had the highest activities. Conversely, calpastatin activity increased linearly (P < .01) with increasing percentage B breeding. These data show strong linear relationships between calpastatin activity (positive), marbling score (negative), and percentage B breeding, suggesting a possible combined effect of these traits on aged tenderness of intermediate Brahman crosses.

This study was conducted to determine the ability of additional ultrasound measures to enhance the prediction accuracy of retail product and trimmable fat yields based on weight and percentage. Thirty-two Hereford-sired steers were ultrasonically measured for 12th-rib fat thickness, longissimus muscle area, rump fat thickness, and gluteus medius depth immediately before slaughter. Chilled carcasses were evaluated for USDA yield grade factors and then fabricated into closely trimmed, boneless subprimals with 0.32 cm s.c. fat. The kilogram weight of end-point product included the weight of trimmed, boneless subprimals plus lean trim weights, chemically adjusted to 20% fat, whereas the fat included the weight of trimmed fat plus the weight of fat in the lean trim. Prediction equations for carcass yield end points were developed using live animal or carcass measurements, and live animal equations were developed including ultrasound ribeye area or using only linear measurements. Multiple regression equations, with and without ultrasound rump fat thickness and gluteus medius depth, had similar R2 values when predicting kilograms of product and percentages of product, suggesting that these alternative variables explained little additional variation. Final unshrunk weight and ultrasound 12th-rib fat thickness explained most of the variation when predicting kilograms of fat. Rump fat and gluteus medius depth accounted for an additional 10% of the variation in kilograms of fat, compared with the equation containing final weight, ultrasound ribeye area, and ultrasound 12th-rib fat thickness; however, the two equations were not significantly different. Prediction equations for the cutability end points had similar R2 values whether live animal ultrasound measurements or actual carcass measurements were used. However, when ultrasound ribeye area was excluded from live animal predictions, lower R2 values were obtained for kilograms of product (0.81 vs 0.67) and percentages of product (0.41 vs 0.17). Conversely, the exclusion of ultrasound ribeye area had little effect on the prediction accuracy for kilograms of fat (0.75 vs 0.74) and percentage fat (0.50 vs 0.40). These data substantiate the ability of live animal ultrasound measures to accurately assess beef carcass composition and suggest that the alternative ultrasound measures, rump fat and gluteus medius depth, improve the accuracy of predicting fat-based carcass yields.

No abstract

Crossbred barrows (n = 144; 80 kg) from four farrowing groups were phenotypically selected into fat (FAT) and lean (LEAN) pens using ultrasound. The difference in 10th-rib fat depth between the LEAN and FAT groups was > or =0.5 cm. Within a farrowing group, pigs were assigned to pens (five pigs per pen and eight pens per phenotype) to equalize pen weight and fat depth. Pigs were fed a corn-soybean meal diet containing 19% CP, 1.0% added animal/vegetable fat, and 1.1% lysine (as-fed basis). Half the pens received 10 ppm (as-fed basis) of ractopamine (RAC) during the 28-d finishing phase. At 7-d intervals, live weight and feed disappearance were recorded to calculate ADG, ADFI, and G:F, and 10th-rib fat depth and LM area were ultrasonically measured to calculate fat-free lean and fat and muscle accretion rates. During the first 7 d on feed, LEAN pigs fed RAC gained less (P < 0.05) than FAT pigs fed RAC or LEAN and FAT pigs fed the control diet (RAC x phenotype; P = 0.02); however, RAC did not (P > 0.25) affect ADG after the second, third, and fourth weeks, or over the entire 28-d feeding period. Although wk-2 and -3 ADG were higher (P < or = 0.03) in LEAN than in FAT pigs, phenotype did not (P = 0.08) affect overall ADG. Dietary RAC decreased (P < or = 0.05) ADFI over the 28-d feeding trial, as well as in wk 2, 3, and 4, but intake was not (P > 0.20) affected by phenotype. Neither RAC nor phenotype affected (P > 0.10) G:F after 7 d on trial; however, RAC improved (P < or = 0.04) wk-3, wk-4, and overall G:F. Lean pigs were more efficient (P < or = 0.05) in wk 2 and 3 and over the duration of the trial than FAT pigs. Ultrasound LM accretion (ULA) was not (P > or = 0.10) affected by RAC; however, LEAN pigs had greater (P < or = 0.02) ULA in wk 2 and 4 than FAT pigs. Although fat depth was lower (P < 0.01) in RAC-fed pigs than pigs fed the control diet, ultrasound fat accretion rate indicated that RAC-pigs deposited less (P = 0.04) fat only during wk 4. In addition, calculated fat-free lean (using ultrasound body fat, ULA, and BW) was increased (P < 0.05) in RAC pigs after 3 and 4 wk of supplementation. In conclusion, RAC enhanced the performance of finishing swine through decreased ADFI and increased G:F, whereas carcass lean was enhanced through decreases in carcass fat and increases in carcass muscling.