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It is well known from laboratory studies that a single mycorrhizal fungal isolate can colonize different plant species, form interplant linkages, and provide a conduit for interplant transfer of isotopic carbon, nitrogen, phosphorus, or water. There is increasing laboratory and field evidence that the magnitude and direction of transfer is influenced by physiological source–sink gradients between plants. There is also evidence that mycorrhizal fungi play a role in regulating transfer through their own source–sink patterns, frequency of links, and mycorrhizal dependency. Although it is plausible that connections are extensive in nature, field studies have been hampered by our inability to observe them in situ and by belowground complexity. In future, isotopic tracers, morphological observations, microsatellite techniques, and fluorescent dyes will be useful in the study of networks in nature. Mycorrhizal networks have the potential to influence patterns of seedling establishment, interplant competition, plant diversity, and plant community dynamics, but studies in this area are just beginning. Future plant community studies would benefit from concurrent experimental use of fungal network controls, isotopic labeling, direct observation of interplant linkages, and long-term observation in the field. In this paper, we review recent literature on mycorrhizal networks and interplant carbon transfer, suggest future research directions, and highlight promising scientific approaches.Key words: common mycorrhizal network, carbon transfer, source–sink, establishment, competition, diversity.

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Summary• Ectomycorrhizal (ECM) fungal communities of Douglas-fir ( Pseudotsuga menziesii ) and paper birch ( Betula papyrifera ) were studied along a chronosequence of forest development after stand-replacing disturbance. Previous studies of ECM succession did not use molecular techniques for fungal identification or lacked replication, and none examined different host species.• Four age classes of mixed forests were sampled: 5-, 26-, 65-, and 100-yr-old, including wildfire-origin stands from all four classes and stands of clearcut origin from the youngest two classes. Morphotyping and DNA sequences were used to identify fungi on ECM root tips.• ECM fungal diversities were lower in 5-yr-old than in older stands on Douglas-fir, but were similar among age classes on paper birch. Host-specific fungi dominated in 5-yr-old stands, but host generalists were dominant in the oldest two age classes. ECM fungal community compositions were similar in 65-and 100-yr-old stands but differed among all other pairs of age classes.• Within the age range studied, site-level ECM fungal diversity reached a plateau by the 26-yr-old age class, while community composition stabilized by the 65-yr-old class. Simple categories such as 'early stage', 'multi stage', and 'late stage' were insufficient to describe fungal species' successional patterns. Rather, ECM fungal succession may be best described in the context of stand development.

Climate warming is leading to shrub expansion in Arctic tundra. Shrubs form ectomycorrhizal (ECM) associations with soil fungi that are central to ecosystem carbon balance as determinants of plant community structure and as decomposers of soil organic matter. To assess potential climate change impacts on ECM communities, we analysed fungal internal transcribed spacer sequences from ECM root tips of the dominant tundra shrub Betula nana growing in treatments plots that had received long-term warming by greenhouses and/or fertilization as part of the Arctic LongTerm Ecological Research experiment at Toolik Lake Alaska, USA. We demonstrate opposing effects of long-term warming and fertilization treatments on ECM fungal diversity; with warming increasing and fertilization reducing the diversity of ECM communities. We show that warming leads to a significant increase in high biomass fungi with proteolytic capacity, especially Cortinarius spp., and a reduction of fungi with high affinities for labile N, especially Russula spp. In contrast, fertilization treatments led to relatively small changes in the composition of the ECM community, but increased the abundance of saprotrophs. Our data suggest that warming profoundly alters nutrient cycling in tundra, and may facilitate the expansion of B. nana through the formation of mycorrhizal networks of larger size.

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Summary• The role of mycorrhizal networks in forest dynamics is poorly understood because of the elusiveness of their spatial structure. We mapped the belowground distribution of the fungi Rhizopogon vesiculosus and Rhizopogon vinicolor and interior Douglas-fir trees (Pseudotsuga menziesii var. glauca) to determine the architecture of a mycorrhizal network in a multi-aged old-growth forest.• Rhizopogon spp. mycorrhizas were collected within a 30 · 30 m plot. Trees and fungal genets were identified using multi-locus microsatellite DNA analysis. Tree genotypes from mycorrhizas were matched to reference trees aboveground. Two trees were considered linked if they shared the same fungal genet(s).• The two Rhizopogon species each formed 13-14 genets, each colonizing up to 19 trees in the plot. Rhizopogon vesiculosus genets were larger, occurred at greater depths, and linked more trees than genets of R. vinicolor. Multiple tree cohorts were linked, with young saplings established within the mycorrhizal network of Douglas-fir veterans. A strong positive relationship was found between tree size and connectivity, resulting in a scale-free network architecture with smallworld properties.• This mycorrhizal network architecture suggests an efficient and robust network, where large trees play a foundational role in facilitating conspecific regeneration and stabilizing the ecosystem.