: Background: Stunting is a condition of toddlers who have less length or height compared to age. Toddler stunting is a chronic nutritional problem caused by many factors, one of which is LBW. Babies born with LBW will be at high risk of morbidity, mortality, infectious diseases, underweight and stunting in the early neonatal period to childhood.The purpose of this study is to determine the association between Low Birth Weight (LBW) with the incidence of stunting in toddlers (aged 24-59 months) in the Way Urang Community Health Center in South Lampung Regency. Method: This study uses an observational analytic method with a case control study design. LBW data is secondary data obtained by looking at the birth weight data of children under five listed in the Maternal and Child Health book Way Urang Community Health Center data. The statistical analysis uses the Chi Square test. Result: There is an association of Low Birth Weight (LBW) with the occurrence of stunting in toddlers (aged 24-59 months) in Way Urang Community Health Center South Lampung Regency. P value= 0,024. Conclusion: There is a significant association of Low Birth Weight with the incidence of stunting in toddlers (aged 24-59 months) in the Way Urang Community Health Center in South Lampung Regency.
Stunting is a condition of chronic malnutrition in children that causes the posture is not optimal and triggers other disorders such as deacreasing of immunity and intelligence. The level of adequacy of zinc is one of the factors that influence the incidence of stunting. Zinc is needed to activate and begin the synthesis of Growth Hormone (GH) so that children with zinc deficiency cause GH receptors to be disrupted and GH production to be resistant. This study aims to examine the decrease in zinc levels by looking at the difference in mean zinc levels in stunting and non-stunting toddlers. The research method was carried out observational analytic with a cross sectional approach used in this study. The study sample was 40 toddlers aged 24-60 months with 18 stunting toddlers and 22 non-stunting toddlers in Gunung Sugih District, Central Lampung Regency. The results of univariate analysis revealed that the average zinc level of toddlers serum was 52.60 ug / dl with the largest value of 76 ug / dl and the lowest value was 24 ug / dl. The results of bivariate analysis showed that there were significant differences in the mean zinc levels of stunting and non-stunting children (p = 0.01). The mean zinc content of stunting infants was 45.06 ± 12.21 lower than the mean zinc level of non-stunting infants 58.77 ± 12.98. There is a decrease in serum zinc levels in stunting toddlers compared to non stunting toddlers.
Santri atau sabun cair antibakteri adalah sabun cair yang diformulasikan dari bahan-bahan sabun dan tanaman herbal antiseptik, daun sirih. Tujuan dari kegiatan pengabdian kepada masyarakat ini adalah memberikan pelatihan pembuatan sabun cair antibakteri berbasis tanaman herbal kepada kelompok PKK Desa Mandah. Metode yang digunakan meliputi pelatihan, pembinaan dan pendampingan pembuatan sabun cair antibakteri. Hasil kegiatan menunjukkan adanya kenaikan dengan rata-rata peningkatan pencapaian TIK (tujuan Instruksional Khusus) peserta rata-rata sekitar 55,1 %. Hasil ini menunjukkan bahwa pengetahuan masyarakat tentang pembuatan sabun cair antibakteri dan cara pembuatannya mengalami peningkatan secara signifikan. Peningkatan pengetahuan ibu-ibu PKK ini diharapkan dapat ditularkan ke masyarakat Desa Mandah secara umum sehingga dapat bersinergi dalam peningkatan kesejahteraan masyarakat dan pemberantasan pengangguran.
ABSTRAKUpaya mempercepat penurunan angka kematian ibu (AKI) dan AKB, Departemen Kesehatan telah melaksanakan berbagai program yang berhubungan dengan kesehatan ibu dan anak dan salah satunya pencegahan tetanus neonatorum. Upaya ini dilaksanakan dengan pencegahan infeksi pada persalinan dan perawatan bayi baru lahir termasuk perawatan tali pusat (Depkes RI,2007). Tujuannya Untuk mengetahui hubungan perawatan tali pusat dengan lama lepas tali pusat pada bayi baru lahir di Puskesmas Lakessi Penelitian ini menggunakan metode observasional dengan menggunakan pendekatan analitik cross sectional, subyek penelitian adalah ibu postpartum yang memiliki bayi usia satu hari sampai lepasnya tali pusat, di Puskesmas Lakessi Kota Parepare. Teknik pengambilan sampel dengan teknik sample jenuh sejumlah 30 responden dengan alat ukur yang digunakan adalah checklist dan lembar observasi, analisis data menggunakan Kendall Tau.Dari penelitian ini diperoleh 19 responden (63,3%) melakukan perawatan tali pusat dengan baik, 8 responden (26,7%) melakukan perawatan dengan kategori cukup dan 3 responden (10%) melakukan perawatan tali pusat dengan kategori kurang, sedangkan untuk lama lepas tali pusat diperoleh 21 responden (70%) dengan waktu lepas sedang, 5 responden (16,7%) dalam kategori cepat, dan 4 responden (13,3%) dalam kategori lama. Analisis uji Kendall Tau diperoleh hasil nilai signifikan 0,012 yang menunjukkan bahwa nilai p value < 0,05. Ada hubungan yang signifikan antara perawatan tali pusat pada bayi baru lahir dengan lama lepas tali pusat. Bagi bidan diharapkan tidak hanya memberikan konseling saja tentang perawatan tali pusat tetapi sebaiknya didemonstrasikan bersama ibu. Kata kunci: Perawatan tali pusat, bayi baru lahir, lama lepas. ABSTRACTAccelerate efforts to reduce maternal mortality rate and infant mortality rate, the department of health has implemented various programs dealing with health mother and child and one of them tetanus neonatorum prevention .This effort implemented with the prevention of infection in childbirth and care of newborn infant including care umbilical cord ( indonesian ministry of finance , 2007 ) .The goal is to know the care relationships the umbilical cord with long off the umbilical cord at a new baby born in puskesmas lakessi From the study obtained 19 respondents ( 63,3 % ) maintenance of the umbilical cord well , 8 respondents ( 26,7 % ) maintenance category 3 respondents enough and ( 10 % ) maintenance of the umbilical cord category less , the long off the umbilical cord obtained 21 respondents ( 70 % ) with time off was , 5 respondents ( 16,7 % ) in a category fast , and 4 respondents ( 13,3 % ) in a category long .The analysis kendall know the results significant value 0,012 indicating that the p value < 0,05 .A significant relation exists between care the umbilical cord in newborn babies with long off the umbilical cord .For the is expected to not only give counseling just about care the umbilical cord but should demonstrated with mrs
Optimization of Western Blot Technique for Protein Expression of Rice Plant (Oryza sativa L.) Western blot (WB) technique has been widely used for analyzing protein expression and for identifying specific proteins derived from animals, plants, and microorganisms. During the use of WB, especially in agricultural studies, some difficulties are encountered such as unclear or unspecific protein bands, the presence of bubbles, and the absence of protein bands on membrane. This study aims to determine the WB conditions appropriate for the protein expression of rice plants (Oryza sativa L.). Protein from rice plant was extracted and the obtained protein lysate was then used for proteomic analysis using western blot with β-actin antibody. Our experiment showed that some optimized parameters like blocking buffers, the concentration of primary antibody and the ratio of secondary antibody determined the clarity of the results. β-actin was used as internal control that measured the success of the WB technique. Results showed that lysis process was important in determining good WB results in addition to the optimal blocking solution using a BSA of 0.2%, a primary antibody concentration of 1 μg mL–1, and a secondary antibody of 1:10,000. Optimizing techniques during extraction, incubation, and documentation facilitated good WB results.Keywords: β-actin; optimization; protein; rice plant; western blotABSTRAKTeknik western blot (WB) telah banyak digunakan untuk analisis ekspresi protein dan mengidentifikasi protein spesifik dari hewan, tumbuhan dan mikroorganisme. Dalam implementasi teknik WB, khususnya studi dalam bidang pertanian, beberapa kesulitan ditemui seperti pita protein tidak jelas, tidak spesifik, adanya gelembung, hingga tidak munculnya pita protein pada membran. Penelitian ini bertujuan untuk mengetahui kondisi WB yang tepat untuk deteksi protein tanaman padi (Oryza sativa L.). Protein tanaman padi diekstraksi, kemudian lysate protein yang didapat dianalisis dengan metode westernblot menggunakan antibody β-actin. Penelitian kami menunjukkan bahwa beberapa parameter yang dioptimasi seperti larutan blocking, konsentrasi antibodi primer dan rasio antibodi sekunder akan menentukan hasil yang jelas. β-actin digunakan sebagai kontrol internal yang menjadi tolok ukur keberhasilan teknik WB. Hasil menunjukkan bahwa proses lisis menjadi hal penting dalam menentukan hasil WB yang baik disamping larutan blocking yang optimal menggunakan BSA 0,2%, konsentrasi antibodi primer 1 µg mL–1 dan antibodi sekunder 1:10.000. Mengoptimalkan teknik selama ekstraksi, inkubasi dan dokumentasi membantu mendapatkan hasil WB yang baik.
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