Penelitian ini bertujuan mengevaluasi pengaruh bubuk tempe instan terhadap kadar malonaldehid (MDA) pada serum tikus hiperglikemik. Sebanyak 25 tikus jantan Spraque Dawley umur dua bulan digunakan dalam penelitian ini. Tikus dibagi atas lima kelompok perlakuan yaitu A (normal + diet standar), B (hiperglikemik + diet standar), C (hiperglikemik + 20% bubuk tempe segar), D (hiperglikemik + 20% bubuk tempe instan), dan E (hiperglikemik + 35% bubuk tempe instan). Tikus dikondisikan menjadi hiperglikemik dengan induksi streptozotocin dosis tunggal 45 mg/kg bb intraperitoneum. Diet tempe diberikan selama 8 minggu. Hasil penelitian memperlihatkan bahwa rata-rata kadar MDA kelompok A; B; C; D; dan E masing-masing adalah 2,16; 3,67; 3,13; 3,08; dan 2,98. Substitusi 35% bubuk tempe instan pada diet standar dapat menurunkan kadar MDA serum tikus hiperglikemik yang paling tinggi.
The objective of this study to evaluate the glucose control potential of instant yellow cornmeal and tempe flour by using hyperglycemic mouse models. In approaching this purpose, 24 male ddY mice were used and divided into 3 goups : group A (positive control group, hyperglycemic, 100 % standard feed), group B (treatment group, hyperglycemic, 60 % instant yellow cornmeal + 40 % instant tempe flour), and group C (negative control group, normal, 100 % standard feed). The evaluated parameters of body weight and blood glucose levels were measured during the 8-week period of the study. Results showed that group B (60 % instant yellow cornmeal + 40 % instant tempe flour ration) indicated the potential to control hyperglycemic conditions (p<0.05 ) and displayed higher levels of body weight (6.05 ± 6.08 grams, p >0.05 ) compared to groups A and C.
This research aimed to explore, isolate, select, and characterize lactic acid bacteria (LAB) potentially used as amylolytic bacteria from traditional fermented food “dadih”. Dadih was collected from Lareh Sago Halaban and Lintau, West Sumatera Province. The LAB was isolated by the streak method to obtain a uniform color and size colonies. The selected isolates were characterized by the reaction gram and catalase test. The result showed that isolates had the form of coccus, coccobacillus, and basil with gram-positive, catalase-negative, and endospore-negative reactions. The further characterization using the KIT API 50 CHL identified Lactobacillus plantarum (mH1), Lactobacillus paracasei ssp paracasei (mL3), Lactobacillus plantarum (mH4), Lactococcus lactis subsp lactis (mH6), Lactobacillus plantarum (mH7), Lactobacillus plantarum (mH8), Lactobacillus brevis (mH13), and Lactobacillus paracasei subsp paracasei (mL14). The amylolytic and starch hydrolysis test showed that the isolates with higher amylolytic index ≥2 were Lactobacillus paracasei ssp paracasei mL3, Lactobacillus plantarum mH4, and Lactobacillus paracasei ssp paracasei mL14. The Lactobacillus plantarum mH8 had a lower amylolytic index of 0.7. Therefore, the four isolates (mL3, mH4, mL14, and mH8) are potentially used as fermenting bacteria for high- carbohydrate food.
The research has been carried out in the Processing and Chemistry Laboratory of Politeknik Pertanian Negeri Payakumbuh and Post Harvest Laboratory in Bogor since July until November 2006. The research objectives was to prolong the using time of used cooking oil on food industries through applying the empty fruit bunch of palm bioadsorbent. The optimum condition of TKKS applied were 125 mm size and 5% bioadsorben from the weight of oil (Desminarti dan Rahzarni, 2004). The prolonging of cooking oil application can be done throughrefining used cooking oil so that the part of bad cooking oil can be lremoved. Statistical design used in this research was Completely Randomized Design with four treatments dan three replications. If the result was significant it will be followed by DMRT test on 5% significant level. Based on the experiment could be concluded that that four times titration could produced the oil based on SNI criterya in the relation to the water content (0.23%), peroxide value (0.82%) and free fatty acid value (0.23%) and it could also decrease Fe content from 76ppm to 22 ppm, Cu from 1.2 ppm to 0.40 ppm and Non Urea Adduct Forming (NAF) from 126 ppm to 102 ppm. The bioadsorbent sorption content on water varied from 78% to 80%; peroxide value from 14.71 to 59.80%, free fattyacid from 55.61 to 89.25%, Fe from 68.42 to 71.05%, Cu from 5% to 60% and NAF from 17.46 to 19.05%.
Heavy metal pollution of drinking water has been a major problem in the worldwide. Indonesian National Standarditaion has set the limits for heavy metals contamination in mineral water and bottled drinking water. This study was designed as a cross-sectional field observational study conducted among six refilled drinking water depots and one bottled drinking water in Limapuluh Kota Regency, West Sumatra Province. This study aimed to analyze the levels of chemical contamination, especially heavy metal contamination, which were Mercury (Hg), Cadmium (Cd), Lead (Pb), Chopper (Cu), and Zinc (Zn). Metal contamination was analyzed in 6 drinking water depots and one bottled drinking water sample using Inductively Coupled Plasma (ICP) spectrophotometry method. The result shows that there are 24 metals contained in the sample. Five types of heavy metals are highly toxic: Hg, Cd, Pb, Cu, and Zn. Refilled drinking water depot samples studied in Harau District area were containing heavy metals that exceeded the standards set in SNI 01-3553-2006 state that 0.005 mg/L for Pb, max. 0.5 mg/L for Cu, max. 0.003 mg/L for Cd, max. 0.001 mg/L for Hg, and no content for Ag and Co. In the result were; Lead (Pb) in sample A (0.0079 mg/L), B (0.0103 mg/L), C (0.0070 mg/L), D (0.0061 mg/L), E (0.0094 mg/L), F and G (0.0073 mg/L); Cadmium (Cd) in sample A (0.0052 mg/L), C (0.0035 mg/L), D (0095 mg/L), E (0.0063 mg/L), F (0.0049 mg/L), and G (0.0059 mg/L). Only one water sample was still at allowed levels of contaminant Cd in sample water B (0.0025 mg/L). While, all samples contained excess Pb. Meanwhile, six water samples contaminated by Cd were within unacceptable limits. While metal contamination such as Hg (not available), Cu (0.0148-0.0038 mg/L), and Zn (0.0293-0.0643 mg/L) are still at the allowed levels.
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