This work describes a method for the determination of theFusarium mycotoxin moniliformin (MON) in cereals. In addition to the optimization of the clean-up and the HPLC determination the most efficient extraction mode was investigated on natural contaminated samples. The method was validated for maize and wheat using a calibration range from 57 to 2300 µg/kg. Due to the ionic nature of the toxin the clean-up of the extracts was carried out with strong-anion-exchange columns. Moniliformin was separated by reversed phase ion-pair-chromatography (RP-Ion pair-HPLC) and detected by DAD. The validated method yielded recoveries of 76%±9% (maize) and 87%±5% (wheat) and detection limits of 39 µg/kg and 30 µg/kg, respectively. The suitability of the developed method was demonstrated on natural contaminated samples.
El estudio del nicho trófico permite conocer aspectos relacionados con el espectro nutricional en el contexto de la población, incluyendo la comparación de las preferencias de los subgrupos sexuales y etarios de la especie, como también las variaciones estacionales. Además, permite conocer características comportamentales en la obtención de los recursos (Aun et al., 1999;Teixeira-Fialho et al., 2003). La descripción y cuantificación de la dieta, son uno de los principales aspectos en los estudios de ecología de las especies (
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