This work describes a method for the determination of theFusarium mycotoxin moniliformin (MON) in cereals. In addition to the optimization of the clean-up and the HPLC determination the most efficient extraction mode was investigated on natural contaminated samples. The method was validated for maize and wheat using a calibration range from 57 to 2300 µg/kg. Due to the ionic nature of the toxin the clean-up of the extracts was carried out with strong-anion-exchange columns. Moniliformin was separated by reversed phase ion-pair-chromatography (RP-Ion pair-HPLC) and detected by DAD. The validated method yielded recoveries of 76%±9% (maize) and 87%±5% (wheat) and detection limits of 39 µg/kg and 30 µg/kg, respectively. The suitability of the developed method was demonstrated on natural contaminated samples.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.