Snake envenomation has been estimated to affect 1.8 million people annually with about 94,000 deaths mostly in poor tropical countries. Specific antivenoms are the only rational and effective therapy for these cases. Efforts are being made to produce effective, affordable and sufficient antivenoms for these victims. The immunization process, which has rarely been described in detail, is one step that needs to be rigorously studied and improved especially with regard to the production of polyspecific antisera. The polyspecific nature of therapeutic antivenom could obviate the need to identify the culprit snake species. The aim of this study was to produce potent polyspecific antisera against 3 medically important vipers of Thailand and its neighboring countries, namely Cryptelytrops albolabris "White lipped pit viper" (CA), Calleoselasma rhodostoma “Malayan pit viper” (CR), and Daboia siamensis “Russell’s viper” (DS). Four horses were immunized with a mixture of the 3 viper venoms using the ‘low dose, low volume multi-site’ immunization protocol. The antisera showed rapid rise in ELISA titers against the 3 venoms and reached plateau at about the 8th week post-immunization. The in vivo neutralization potency (P) of the antisera against CA, CR and DS venoms was 10.40, 2.42 and 0.76 mg/ml, respectively and was much higher than the minimal potency limits set by Queen Soavabha Memorial Institute (QSMI). The corresponding potency values for the QSMI monospecific antisera against CA, CR and DS venoms were 7.28, 3.12 and 1.50 mg/ml, respectively. The polyspecific antisera also effectively neutralized the procoagulant, hemorrhagic, necrotic and nephrotoxic activities of the viper venoms. This effective immunization protocol should be useful in the production of potent polyspecific antisera against snake venoms, and equine antisera against tetanus, diphtheria or rabies.
The ability of monovalent IgG and F(ab')2 antivenoms to neutralize lethality, phospholipase A2, and coagulant activities induced by Daboia siamensis venom was studied. Both antivenoms were produced from the same batch of hyperimmune horse plasma and were adjusted to the same potency against the lethal effect of D. siamensis venom in experiments involving preincubation of venom and antivenom. Intact neutralization experiments involving independent injection of venom and antivenoms showed that the F(ab')2 antivenom was slightly more effective. Significant differences in favour of F(ab')2 antivenom were observed with respect to neutralization of phospholipase A2 and coagulant activities. Both IgG and F(ab')2 antivenoms were able to activate human complement in vitro. IgG antivenom had a significantly higher anticomplementary activity than F(ab')2 antivenom.
Characterization and quantification of cytokine production is essential for understanding the immune response. A SYBR Green real-time quantification Reverse Transcriptase-Polymerase Chain Reaction (RT-qPCR) was used to measure mRNA expression levels of the most common cytokines in horses after cobra snake venom stimulated Peripheral Blood Mononuclear Cells (PBMC) carried out by in vitro method. The PBMC from horse was stimulated with cobra snake venom (10 μg mLG 1) prior to incubate at 3, 6, 24 and 48 h. Cytokine mRNA expression level, IL-1β, IL-10, IFNγ and TNFα, were measured using SYBR Green real-time RT-PCR. The highest mRNA expression of IL-1β and IL-10 were demonstrated at 3 h after stimulation of cobra snake venom. The levels of IL-1β and IL-10 expression were 0.7 and 2.3 folds. Meanwhile, the mRNA expression of IFNγ and TNFα were peaked at 6 and 24 h with appreciable increment of 13 and 5.4 folds, respectively. However, the mRNA expression of these cytokines was decreased after 24 h. It seems likely that cobra snake venom could stimulate more mRNA expression of IFNγ than IL-1β, IL-10 and TNFα in PBMC of horse. Secretion of IFNγ is likely to be important in early host defense against pro-inflammation and tissue injury from cobra snake venom. Thus, the study of mRNA expression of inflammatory cytokine profiles in this model could provide information useful for understanding the immunopathological mechanism of inflammation from snake venom immunization in horses and led to improve the design and manufacture of snake anitvenom.
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