People have been searching toward to biofuel as a prominent alternative to replace oil fuel. Microalgae biomass, specifically from the genus Synechococcus, has been used as the biofuel’s feedstock as it’s able to grow faster than the other groups at cyanobacteria. Strains being used were isolated from Ciseeng hot spring (HS-7) and Rawa Danau Banten hot spring (HS-9). The intensity of aeration (IA) were given as high (330 bubbles/min) and low (220 bubbles/min) to understand its influence on feedstock growth. Change in growth was observed microscopically using direct methods over fourteen days. At the end of the experiment, result demonstrate that high IA gaves rise to Synechococcus biomass density at 125,21 × 106 cell/ml and 100,63 × 106 cell/ml for HS-7 & HS-9 respectively. Meanwhile, low IA gaves rise to Synechococcus biomass density at 21,7 × 106 cell/ml and 10,61 × 106 cell/ml for HS-7 & HS-9 accordingly. Positive trend also follows the growth at Synechococcus biomass with high IA, but did not apply to the system with low IA. The difference in biomass production occurred because of discrepancies in cell and nutrient distribution inside the photobioreactor system. Therefore, it implies that proper controlling of IA would affect the yielding of feedstock for producing biofuels.
Photobioreactor (PBR) have been one of the most popular system that used for multiplying microalgal biomass. Many researches have been conducted to develop photobioreactor that suit the most with prominent microalgae strains. Carbon dioxide supply is one of many sectors that play an important role for growing microalgae. Carbon dioxide are used by the microalgal cells to produce many chemical compounds that support growth. In many cases, carbon dioxide must be solved into growth media to be utilized by the cells. This research focused on analyzing the effect of carbon dioxide solubility increasement caused by variation of baffle in airlift photobioreactor (APBR) to growth rate of Synechococcus HS-9 biomass. Twenty-one day of experiment resulting that there are growth rate differences occurs. The highest growth rate occurs in APBR-A, APBR-B, and control PBR are 0.515, 0.463, and 0.738 respectively. Despite of the maximum growth rate occurred, there are fluctuation of growth rate happen during experiment. Carbon dioxide solubility test then conducted to see effect of baffle variation. The best carbon dioxide solubility occurred in APBR-B with 23 mg/L, then followed by APBR-A with 22 mg/L, and control PBR with 20 mg/L after 24 hours of treatment. Further analysis resulting that there is no strong correlation between carbon dioxide solubility increasement to growth rate of Synechococcus HS-9 biomass. This occurred due degression of acidity value (pH) caused by excess of carbonic acid formed by carbon dioxides who diluted into growth media.
Indonesia known as a hotspot of biodiversity, including cyanobacteria biodiversity. One member of cyanobacteria (prokaryotic algae) is Leptolyngbya. Leptolyngbya HS-16 is an isolate that had been isolated from hot spring in Red Crater of Gunung Pancar, Sentul, Bogor. As mats-producing microalgae, this strain is a very promising source of Biofuel. Biofuel can be extracted from lipid of microalgal biomass. Bioreactor is a method to encourage the growth of microalgal biomass. To get a best result in growth, agitation must be done, to make sure every single cell of microalgae gets the adequate nutrition. The aeration on simple photobioreactors is set to high and low intensity. The high intensity of aerations average amount are 191 bubble/min, while the low intensity one are 117 bubble/min, with a device that could produce smaller bubble size to reduce the aeration-agitation effect. The research was done to acknowledge the effect of aeration intensity to Leptolygnbya HS-16.
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