Tubular Photobioreactor: A Preliminary Experiment Using Synechococcus sp. (Cyanobacteria) Cultivated in NPK Media for Biomass Production as Biofuel Feedstock

Photobioreactor (PBR) have been one of the most popular system that used for multiplying microalgal biomass. Many researches have been conducted to develop photobioreactor that suit the most with prominent microalgae strains. Carbon dioxide supply is one of many sectors that play an important role for growing microalgae. Carbon dioxide are used by the microalgal cells to produce many chemical compounds that support growth. In many cases, carbon dioxide must be solved into growth media to be utilized by the cells. This research focused on analyzing the effect of carbon dioxide solubility increasement caused by variation of baffle in airlift photobioreactor (APBR) to growth rate of Synechococcus HS-9 biomass. Twenty-one day of experiment resulting that there are growth rate differences occurs. The highest growth rate occurs in APBR-A, APBR-B, and control PBR are 0.515, 0.463, and 0.738 respectively. Despite of the maximum growth rate occurred, there are fluctuation of growth rate happen during experiment. Carbon dioxide solubility test then conducted to see effect of baffle variation. The best carbon dioxide solubility occurred in APBR-B with 23 mg/L, then followed by APBR-A with 22 mg/L, and control PBR with 20 mg/L after 24 hours of treatment. Further analysis resulting that there is no strong correlation between carbon dioxide solubility increasement to growth rate of Synechococcus HS-9 biomass. This occurred due degression of acidity value (pH) caused by excess of carbonic acid formed by carbon dioxides who diluted into growth media.

Section: Methods and Experimental Setupmentioning

confidence: 99%

Analysis of Carbon Dioxide Solubility Increasement Caused by Baffle Diameter Variation in Airlift Photobioreactor to Growth Rate of Synechococcus HS-9 Biomass

Ardiansyah¹,

Nasruddin²,

Sjamsuridzal³

et al. 2021

“…NPK fertilizer is dissolved depending on the concentration to be made. A concentration of 80 ppm is produced by dissolving 80 mg of NPK commercial fertilizer into 1000 mL 28) . The same ways are used to obtain concentrations of 160 ppm and 240 ppm.…”

Section: Manufacture Of Bbm and Npk Mediummentioning

confidence: 99%

Biomass Production of Indonesian Indigenous Leptolyngbya Strain on NPK Fertilizer Medium and its Potential as a Source of Biofuel

Prihantini¹,

Rakhmayanti²,

Handayani³

et al. 2020

Leptolyngbya is one of the genera of Cyanobacteria (prokaryotic microalgae) which are often found in the hot spring area. These microalgae are known to have potential as a biofuels source. In order to utilize microalgae, it is necessary to make efforts to produce microalgae biomass in large quantities. Biomass production depends on many environmental factors, one of which is the medium. The use of appropriate media and cheaply be one option, since biomass must be produced in large scale. Therefore, research on variations in the concentration of NPK fertilizer medium against the growth of Leptolyngbya HS-16 compared to Bold Basal Medium (BBM) was carried out. NPK fertilizer medium (nitrogen/N, phosphorus/P and potassium/K) is an inexpensive microalgae growth medium. Leptolyngbya HS-16 or Leptolyngbia with strain code of HS-16 is a Leptolyngbia strain isolated from a habitat in the hot springs of Mount Pancar Crater in Indonesia. The purpose of the study was to determine the effect of NPK medium concentration on the biomass weight of Leptolyngbya HS-16. The medium used in the study was BBM as the control medium, and NPK media with various concentrations of 80 ppm, 160 ppm, and 240 ppm as the treatment medium. In addition, the study aimed to determine the appropriate concentration of NPK medium to produce high biomass weight, supported by high lipid content. The results showed that Leptolyngbya HS-16 grew better in NPK medium with a concentration of 80 ppm compared to other concentrations. This can be seen from the results of the average biomass produced at peak time of 3.008 mg. L -1 and the log length of the Leptolyngbya HS-16. The lipid content was produced in the 80 ppm NPK medium was 45%.

“…Beside of that, people also start using biofuel that can be produced from plants biomass like sugarcane and corn 4) . Besides plants biomass, biofuel also can be produced from microorganism biomass 5) . Microorganisms that can be used to produce biomass as a basic material for biofuels are microalgae.…”

Section: Introductionmentioning

confidence: 99%

Biomass Production and Lipid Content of Leptolyngbya HS-16 grown in Bubble Column Photobioreactors (BCPBR) with Air Bubbler Pore Variation

Orlando¹,

Nasruddin²,

Sjamsuridzal³

et al. 2021

Research on the production of biomass and lipid content of Leptolyngbya HS-16 grown in a photobioreactor with variations in the number of air bubbles forming holes in the photobioreactor has been carried out. Photobioreactor (PBR) is a system designed to support the life of microorganisms in the system by providing various factors that can be used for growth such as light, carbon dioxide and nutrients. Bubble column photobioreactor (BCPBR) provides aeration for mixing nutrients and a carbon dioxide source for culture. In this study, two types of bubble column photobioreactor (BCPBR) were used with variations in the number of air bubbles, namely 12 (BCPBR1) and 24 (BCPBR2). In addition, TPBR without aeration was used as a control. This study used Leptolyngbya HS-16, an indigenous cyanobacteria isolated from Red Crater, Pancar Mount, West Java. Leptolyngbya HS-16 was inoculated on a bubble column photobioreactor (BCPBR). The lipids obtained from this strain were 4.41% (BCPBR1) and 1.30% (BCPBR2) after 24 days.