Considering the significance in survival and virulence, we have made an attempt to understand modulations in the membrane and cell wall properties of Candida albicans hyphae induced by temperature (37 °C) and neutral pH and yeast form cells grown under low hydrostatic pressure (LHP). Our results suggest that cell surface hydrophobicity (CSH) and adhesion are dynamic properties determined largely by the microenvironment rather than morphological forms, citing the significance of variation in niche specific virulence. GC-MS analysis showed that 49 and 41 fatty acids modulated under hyphal form induced by temperature alone (37 °C) and neutral pH, respectively while that of 58 under yeast form cells under low hydrostatic pressure (LHP) (1800 Pa). Fatty acid and ergosterol data indicates that fluidity increases with increase in temperature (37 °C) and neutral pH i.e., saturated fatty acids and ergosterol decreases. Similarly, CSH and adhesion decrease in response to temperature (37 °C), pH 7, and LHP compared to controls, irrespective of morphological forms. In general, membranes were more rigid, and cell walls were more hydrophobic and adhesive in yeast form compared to hyphal form cells, except in case of yeast form cells grown under LHP. Yeast form cells grown under LHP are less hydrophobic and adhesive.
Polymorphic yeast, Candida albicans, forms thick-walled structures called chlamydospores in order to survive under adverse conditions. We present proteomic profile changes occurring during chlamydospore formation. Chlamydospores were induced by inoculating C. albicans cells (grown for 48 h) on rice extract and semisolid agar containing tween 80 (1%), and were overlaid by a polyethene sheet to induce microaerophilic conditions at 30 • C. Proteins extracted from chlamydospores and hyphae (producing chlamydospores) were identified by LC-MS/MS analysis. Present datasets include proteomic data (Swath spectral libraries) of chlamydospores and yeast phase cells, as well as methodologies and tools used for the data generation. Further analysis is expected to provide an opportunity to understand modulations in metabolic processes, molecular architecture (i.e., cell wall, membrane, and cytoskeleton) and stress response pathways leading to chlamydospore formation and thus facilitating survival of C. albicans under adverse conditions. Data Set: http://www.peptideatlas.org/PASS/PASS01061 (Username: PASS01061, Password: PF8546i) Data Set License: There is no specific license.
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