Proteome analysis of Candida albicans cells undergoing chlamydosporulation

Ingle, Sujata; Kazi, Rubina; Patil, Rajendra; Zore, Gajanan

doi:10.1007/s42485-019-00024-8

Cited by 3 publications

(2 citation statements)

References 96 publications

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“…These data shed light on adaptations that contribute to C. albicans pathobiology and indicated that survival to salt stress involves a transient reduction in ribosome biogenesis and translation, together with the accumulation of glycerol, a key osmolyte for this yeast. Still in C. albicans , Ingle et al [ 280 ] investigated proteins specific to chlamydospore, a unique non-virulent morphological form induced in response to stresses and occasionally found in tissue samples. SWATH-MS analysis revealed morpho-physiological changes in cellular architecture such as cell wall thickening, that enable survival under hostile micro-environments.…”

Section: Proteomics Advances In Ascomycotamentioning

confidence: 99%

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

Muggia

Ametrano

Sterflinger

et al. 2020

Life

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Fungi are among the most successful eukaryotes on Earth: they have evolved strategies to survive in the most diverse environments and stressful conditions and have been selected and exploited for multiple aims by humans. The characteristic features intrinsic of Fungi have required evolutionary changes and adaptations at deep molecular levels. Omics approaches, nowadays including genomics, metagenomics, phylogenomics, transcriptomics, metabolomics, and proteomics have enormously advanced the way to understand fungal diversity at diverse taxonomic levels, under changeable conditions and in still under-investigated environments. These approaches can be applied both on environmental communities and on individual organisms, either in nature or in axenic culture and have led the traditional morphology-based fungal systematic to increasingly implement molecular-based approaches. The advent of next-generation sequencing technologies was key to boost advances in fungal genomics and proteomics research. Much effort has also been directed towards the development of methodologies for optimal genomic DNA and protein extraction and separation. To date, the amount of proteomics investigations in Ascomycetes exceeds those carried out in any other fungal group. This is primarily due to the preponderance of their involvement in plant and animal diseases and multiple industrial applications, and therefore the need to understand the biological basis of the infectious process to develop mechanisms for biologic control, as well as to detect key proteins with roles in stress survival. Here we chose to present an overview as much comprehensive as possible of the major advances, mainly of the past decade, in the fields of genomics (including phylogenomics) and proteomics of Ascomycota, focusing particularly on those reporting on opportunistic pathogenic, extremophilic, polyextremotolerant and lichenized fungi. We also present a review of the mostly used genome sequencing technologies and methods for DNA sequence and protein analyses applied so far for fungi.

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Section: Proteomics Advances In Ascomycotamentioning

confidence: 99%

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

Muggia

Ametrano

Sterflinger

et al. 2020

Life

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“…This leads to cell impermeability, adhesion, and the formation of biofilms to survive under stress conditions [ 49 ]. In addition, during starvation and other stress conditions that affect C. albicans , the chlamydosporulation process is triggered, promoting the generation of chlamydospores, which are cells with a much thicker cell wall, which would make the entry of H. pylori difficult [ 60 ]. In addition, the loss of mobility and of bacterial viability can explain the low percentage of Y-BLBs found in SS co-cultures.…”

Section: Discussionmentioning

confidence: 99%

Nutrient Deficiency Promotes the Entry of Helicobacter pylori Cells into Candida Yeast Cells

Sánchez-Alonzo

Silva-Mieres

Arellano-Arriagada

et al. 2021

Biology

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Helicobacter pylori, a Gram-negative bacterium, has as a natural niche the human gastric epithelium. This pathogen has been reported to enter into Candida yeast cells; however, factors triggering this endosymbiotic relationship remain unknown. The aim of this work was to evaluate in vitro if variations in nutrient concentration in the cultured medium trigger the internalization of H. pylori within Candida cells. We used H. pylori–Candida co-cultures in Brucella broth supplemented with 1%, 5% or 20% fetal bovine serum or in saline solution. Intra-yeast bacteria-like bodies (BLBs) were observed using optical microscopy, while intra-yeast BLBs were identified as H. pylori using FISH and PCR techniques. Intra-yeast H. pylori (BLBs) viability was confirmed using the LIVE/DEAD BacLight Bacterial Viability kit. Intra-yeast H. pylori was present in all combinations of bacteria–yeast strains co-cultured. However, the percentages of yeast cells harboring bacteria (Y-BLBs) varied according to nutrient concentrations and also were strain-dependent. In conclusion, reduced nutrients stresses H. pylori, promoting its entry into Candida cells. The starvation of both H. pylori and Candida strains reduced the percentages of Y-BLBs, suggesting that starving yeast cells may be less capable of harboring stressed H. pylori cells. Moreover, the endosymbiotic relationship between H. pylori and Candida is dependent on the strains co-cultured.

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Opaque cell-specific proteome ofCandida albicansATCC 10231

et al. 2023

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Candida albicans, a polymorphic opportunistic pathogen of humans, can exist in different morphological forms like yeast, hyphae, pseudohyphae, chlamydospores and white and opaque cells. Proteomic analysis of opaque form of C. albicans ATCC 10231 is carried out in the present study using micro-LC-MS/MS and validated using expression analysis of selected genes using RT-qPCR and mitochondrial membrane potential (MMP) assay. This is the first report identifying opaque cell-specific proteins of C. albicans. A total of 188 proteins were significantly modulated under opaque form compared to white cells, of which 110 were up-regulated, and 78 were down-regulated. It was observed that oxidative phosphorylation (OxPhos) and oxidative stress are enhanced in C. albicans cells growing under opaque form as proteins involved in OxPhos (Atp1, Atp3, Atp16, Atp7, Cox6, Nuc2, Qcr7 and Sdh12) and oxidative stress response (Gcs1, Gtt11, Gpx2, Sod1, Ccp1 and Lys7) were significantly up-regulated. The maximum up-regulation of 23.16 and 13.93-fold is observed in the case of Ccp1 and Nuc2, respectively. The down-regulation of proteins viz. Als1, Csh1, Sap9 and Rho1 determining cell surface chemistry indicates modulation in cell wall integrity and reduced adhesion of opaque cells compared to white cells. This study is significant as it is the first draft of the proteomic profile of opaque cells that suggests enhanced OxPhos, oxidative stress, and modulation in cell surface chemistry that indicates reduced adhesion and cell wall integrity that could be associated with reduced virulence in opaque form. However, a deeper investigation is needed to explore it further.

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Proteome analysis of Candida albicans cells undergoing chlamydosporulation

Cited by 3 publications

References 96 publications

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

Nutrient Deficiency Promotes the Entry of Helicobacter pylori Cells into Candida Yeast Cells

Opaque cell-specific proteome ofCandida albicansATCC 10231

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