The objectives of this research were to find out the influence and optimal different shading on growth, nitrogen, carotenoid, and chlorophyll content of Gynura procumbens leaf. Research was carried out in the greenhouse of Faculty of Agriculture Sebelas Maret University and Central Laboratory Sebelas Maret University Surakarta, in June until October 2003. Completely randomized design with single factor was used as follow: 0%, 40%, and 70% shading with 10 replications each treatment. Observation including growth parameters (plant height, surface leaf’s area, dry weight) and the contents of nitrogen, chlorophyll, and carotenoid. Data collected were analyzed using analysis of variance (ANOVA) followed by DMRT test at 5% confidence level and regression test. The result indicated that 40% shading had proven to increase growth parameters, 70% shading giving significant effect on nitrogen and chlorophyll content, while 0% shading showed to increase carotenoid content.
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