ABSTRAKPesatnya perkembangan ilmu dan teknologi menjadikan bioteknologi sebagai salah satu bidang ilmu dalam biologi yang harus dikuasai sejak dini oleh siswa. Berbagai permasalahan yang muncul dalam pembelajaran bioteknologi di sekolah, salah satunya adalah penggunaan media yang kurang tepat sehingga menyebabkan materi tersebut sulit dipahami oleh siswa. Masalah lain adalah adanya keterbatasan waktu, sementara materi yang disampaikam lebih banyak bersifat aplikatif dan abstrak terutama pada bioteknologi modern yang membutuhkan waktu relatif panjang. Tujuan dari penelitian pengembangan ini adalah mengembangkan media pembelajaran berbasis multimedia interaktif untuk meningkatkan pemahaman materi bioteknologi modern pada siswa kelas XII SMA. Model penembangan yang digunakan mengacu pada langkah-langkah pengembangan Borg & Gall (1983) yang dimodifikasi dari Sukmadinata (2009) yang meliputi tiga kegiatan besar, yaitu: studi pendahuluan, pengembangan dan validasi. Penelitian ini dilakukan pada tanggal 19 Januari sampai 28 Maret 2013 di MAN Malang 2 Batu. Hasil penelitian pengembangan ini menghasilkan produk multimedia interaktif yang telah melalui proses uji coba dan telah dilakukan beberapa kali revisi berdasarkan saran ahli materi dan ahli media serta komentar dari siswa sasaran uji coba. Multimedia interaktif yang dihasilkan memiliki kualifikasi sangat baik dan hasil eksperiment juga menunjukkan bahwa terdapat perbedaan tingkat pengetahuan materi bioteknologi modern antara sebelum dan setelah menggunakan multimedia interaktif, yaitu dari nilai ratarata sebelum menggunakan media (pretest) (54,03) meningkat menjadi (92,36) setelah menggunakan media (postest).
Forensics has become an essential part of the disclosure of criminal evidence. A bioinformatics approach in the form of DNA forensics and digital forensics can be a good combination in disclosing digital-based criminal evidence. This study explains how the role of bioinformatics through the digital approach can be a means of forming new approaches in integration with digital forensics, called cyber-bioinformatics. Despite many hopes and challenges ahead, it does not rule out the possibility of criminal cases related to the privacy of human genomic data, so it proposes a new hypothesis, “cyber-bioinformatics.” The role of cyber-bioinformatics is very central in this regard.
Abstract. Riani S, Prabowo RE, Nuryanto A. 2021. Molecular characteristics and taxonomic status of morphologically similar barnacles (Amphibalanus) assessed using the cytochrome c oxidase 1 gene. Biodiversitas 22: 1456-1466. Amphibalanus variegatus and A. reticulatus have similar external morphology. Morphological similarities can be a severe problem for direct species-level identification. The problem can be overcome through anatomy-based identification and validated through molecular barcoding. Molecular characterization using the cytochrome c oxidase 1 (COI) gene provides a useful tool for precise species identification. This study attempted to assess the molecular characteristics of morphologically similar barnacle (Amphibalanus) specimens collected at five localities in Indonesia to validate their taxonomic status. Forty-five barnacle specimens were collected during the field trips in Lampung, Jakarta, Semarang, Bali, and Lombok. The COI gene was amplified using LCO1490 and HCO2198 primers. The gene was sequenced using bidirectional sequencing at 1st base Asia. The specimens' taxonomic status was determined based on sequence identity, genetic distance, monophyly, nucleotide compositions, and nucleotides in a particular position. Shell shapes-based identification placed barnacle specimens into A. reticulatus. However, anatomical-based identification placed barnacle samples into two different anatomic groups, which was further validated by molecular data that two anatomic groups of Amphibalanus samples have significant differences in their COI gene. Based on the molecular characteristics, 43 samples were identified as A. reticulatus, while the two remaining samples were identified as A. variegatus.
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