Crataeva magna and Euphorbia nerifolia have been shown to possess hepatoprotective activity and antioxidant property. The present works with these plants were undertaken with the premise that the drug promoting hepatoprotective activity and radical scavenging property could have effect on wound healing also. The wound healing property of the methanolic extract of the leaves of Crataeva magna (CNM) and Euphorbia nerifolia (ENM) were chosen to investigate in excision and incision wound models. The methanolic extracts of the two plants at the dose of 500 mg/kg/day by topically applying method. Healing was assessed by the rate of wound contraction, time until complete epithelialization, incision breaking strength, estimation of hydroxyproline and histopathological parameters. Complete wound contraction was shown by both the plants in the study period. In excision and incision wound models, all the test drugs showed significant (P<0.001) wound healing activities compared to the control. Moreover the CNM was found to possess significant wound healing activities than the ENM and had been observed to have equipotent wound healing activity as of the standard drug Framycetin.

Objective: These studies focus on the interaction between two clinically active antiparkinsonian drugs L-dopa (L) and carbidopa (C) with the cis-[Cr(C2O4)2(H2O)2] - Methods:The resulting synthesized complexes were characterized by UV-VIS and FTIR spectroscopy. Evaluation of antioxidant activities of this cis-[Cr(C and evaluation of the synthesized product from a coordination chemistry aspect with respect to the possibility of its antioxidant activity and its therapeutic application in the treatment of Parkinson disease. Results:The results of spectral analysis of the synthesized products indicate that complexes have a Cr(III) ion coordinated via the carboxylic and amino group. In the reduction of radical DPPH -carbidopa(MC) and standard butylated hydroxytoluene (BHT) were carried out by using 1,1-diphenyl-1-picrylhydrazyl free radical (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cations and hydrogen peroxide method.· and the formation of radical monocation ABTS ·+ the ability to scavenge radical was measured in these experiments by the discoloration of the solution. However, in hydrogen peroxide method, the increased in absorbance showing its scavenging potential. The scavenging capacity of the test compounds and standard on the DPPH, ABTS ·+ , H2O2 decreased in the order BHT>ML>MC>C>L which were 98.4, 96.4, 86.4, 68.3, 49.7% for DPPH, BHT>ML>L>MC>C which were 99.3, 96.9, 96.3,66.6, 53.4% for ABTS ·+ , BHT>ML>MC>L>C which were 68.8%, 52.4%, 49.6%, 43.1% and 37.7% for H2O2 Conclusion:The experimental findings showed that cis-[Cr(C at the concentration of 50 µg/ml, respectively.

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Bacopa monnieri and Convolvulus pluricaulis with the Panchagavya ghrita are expected to have better nootropic activity than the combination of Bacopa monnieri and Convolvulus pluricaulis. These marshy herbs are found throughout India having various medicinal importances like treatment of ulcer, epilepsy, memory improvement. The present study was focused on the exploration and assessment of memory and learning activity from the aqueous and methanol extract of Bacopa monnieri (Brahmi) and Convolvulus pluricaulis (Sankhapuspi) leaves with and without the Panchagavya ghrita (PGG) by performing different pharmacological screening models. The study of nootropic activity was performed by using different screening methods such as; phenobarbitone sodium-induced sleeping time, elevated plus maze, radial arm maze, actophotometer. Both methanol and aqueous extracts of Brahmi, Sankhapuspi with the combinations of Panchagavya ghrita showed a significant increase in memory and learning activity as compared to solvent control group where methanol extract containing combination exhibited better enhancement of memory learning activity in all experimental models as compared to aqueous extract. However, it is confirmed that herbs are having excellent activities in learning and memory when combined with Panchagavya ghrita.

Drug discovery process develops faster due to more advances in computational techniques. The protein ligand interaction well predicted due to the in-silico approach study. The present investigation focused towards the development of lead structure for treatment of hepatic disorders. An increase in serum acid hydrolase, includingglucuronidase has been reported in numbers of pathological conditions such as arthritis, renal diseases and epilepsies. Enhancement of this enzyme-glucuronidase in blood has been found to correlate significantly with liver damage.glucuronidase inhibitor is a novel approach which is different from the available hepatoprotective drug therapies. Method: The current study is based on in-silico ligand screening and in-vitro estimation of the three flavonols [Naringenin, Quercetin and 2-(3, 4-Dihydroxy Phenyl)-7-Hydroxy-3-(2-Hydroxy Ethoxy) 4-H-Chromen-4one] compounds with enzyme-glucuronidase. Molecular docking software Py Rex and Py Mol was used to dock the selected ligand in the binding site of the crystal structure of protein. Results: Docking results are based on the least binding energy of the selected flavonols compounds. Further attempt has been made towards in-vitro estimation of this enzyme with those selected compounds. The binding affinity with existence of hydrogen bonds leads to find out the mechanism which was well correlated with the findings of in-vitro inhibitory activity. Conclusion: The result outcome of the binding orientation of 2-(3, 4-Dihydroxy Phenyl)-7-Hydroxy-3-(2-Hydroxy Ethoxy) 4-H-Chromen-4one linked with the active amino acid residue of the protein and the binding affinity leads to find out the mechanism for its potential in-vitro inhibitory activity.

Chromatography is the procedure for analysing a multicomponent mixture of trace, minor or major constituent by separaationinto its individual fractions.For the analytical determination of sildenafil in pharmaceutical dosages forms an expeditious & sensitive RP-HPLC method is described.The mobile phase composition for chromatographing sildenafil was acetonitrile: methanol: water(60:25:15, V/V/V) with a flow rate of 1mL/min using C18 column. The internal standard selected was racecadotril and its eluents were observed at 217nm. The retention time was 4.919 min for the drug. Between 5-200μg/mL the linearity of this method was observed. For analysis of drug in tablets, the depicted method was found to be appropriate. The results of the analysis was evaluated statistically. The method were found to be robust, accurate and economic with a major advantage of column longevity, as buffer was not used throughout the method. Hence the proposed method can be extended for routine analysis.

The substitution reaction of cis-[Cr(C2O4)2(H2O)2]-with L-Dopa in aqueous medium has been studied over the range 35 ≤ t ≤ 50 °C, 3.5 (KNO3). The reaction takes place via an outer sphere association between cis-[Cr(C2O4)2(H2O)2]-and L-Dopa followed by chelation. Characterization of the product was done by physicochemical and infrared spectroscopic methods. The antiparkinsonian activity of L-Dopa was found to be less than that of the product chromium(III) complex.

Background: Olax psittacorum, a flowering plant grown in open forests, is consumed by the locals as dietary sources. Aim: This study was designed to check the phytochemical screening, antioxidant activity, antimicrobial studies and Chorioallantoic Membrane (CAM) study in the methanolic extract of Olax psittacorum. Further, in silico docking study was performed on the basis of GC-MS report of the methanolic extract. Methods: Antimicrobial activity was tested against five bacterial strains Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Shigella flexneri, Straphylococcus aureus. In silico docking study was done by two software, Patchdock followed by Firedock and HEX 8.0.0. to find the best fit receptor ligand docked structure. Results: Methanolic extract of Olax psittacorum has exhibited appreciable antioxidant activity. The study showed that the methanolic extract of Olax psittacorum (10mg/ml) with DMSO shows very less activity against Escherichia coli, Salmonella typhi and did not show any activity in Pseudomonas aeruginosa, Shigella flexneri, Staphylococcus aureus. The result of the CAM study showed that the extract has good antiangiogenic activity. The methanolic extract containing phytoligand 1, 2 -Benzenedicarboxylic acid, butyl octyl ester against salivary peroxidase shows maximum Global energy of -69.40 kcal/mol. Conclusion: This study attempted a new dimension for establishing phytochemicals present over methanolic extract of Olax psittacorum which may be useful for mouth ulcer prevention. Science and technology are growing faster to make up things much better but is still lagging with some issues. In vitro and in vivo models help us in determining many facts about plant and its constituent but still it also has many drawbacks. In an intention to overcome the drawbacks an economic and moreover not harming any life creature CAM studies were performed.