In recent years, many methods have been investigated to improve imaging speed in photoacoustic microscopy (PAM). These methods mainly focused upon three critical factors contributing to fast PAM: laser pulse repetition rate, scanning speed, and computing power of the microprocessors. A high laser repetition rate is fundamentally the most crucial factor to increase the PAM speed. In this paper, we review methods adopted for fast PAM systems in detail, specifically with respect to light sources. To the best of our knowledge, ours is the first review article analyzing the fundamental requirements for developing high-speed PAM and their limitations from the perspective of light sources.

Cancer theragnosis agents with both cancer diagnosis and therapy abilities would be the next generation of cancer treatment. Recently, nanomaterials with strong absorption in near-infrared (NIR) region have been explored as promising cancer theragnosis agents for bio-imaging and photothermal therapy (PTT). Herein, we reported the synthesis and application of a novel multifunctional theranostic nanoagent based on hyaluronan (HA)-coated FeOOH@polypyrrole (FeOOH@PPy) nanorods (HA-FeOOH@PPy NRs) for photoacoustic imaging (PAI)-guided PTT. The nanoparticles were intentionally designed with rod-like shape and conjugated with tumor-targeting ligands to enhance the accumulation and achieve the entire tumor distribution of nanoparticles. The prepared HA-FeOOH@PPy NRs showed excellent biocompatible and physiological stabilities in different media. Importantly, HA-FeOOH@PPy NRs exhibited strong NIR absorbance, remarkable photothermal conversion capability, and conversion stability. Furthermore, HA-FeOOH@PPy NRs could act as strong contrast agents to enhance PAI, conducting accurate locating of cancerous tissue, as well as precise guidance for PTT. The in vitro and in vivo photothermal anticancer activity results of the designed nanoparticles evidenced their promising potential in cancer treatment. The tumor-bearing mice completely recovered after 17 days of PTT treatment without obvious side effects. Thus, our work highlights the great potential of using HA-FeOOH@PPy NRs as a theranostic nanoplatform for cancer imaging-guided therapy.

Optical-resolution photoacoustic microscopy (OR-PAM), which has been widely used and studied as a noninvasive and in vivo imaging technique, can yield high-resolution and absorption contrast images. Recently, metallic nanoparticles and dyes, such as gold nanoparticles, methylene blue, and indocyanine green, have been used as contrast agents of OR-PAM. This study demonstrates real-time functional OR-PAM images with high-speed alternating illumination at 2 wavelengths. To generate 2 wavelengths, second harmonic generation at 532 nm with an LBO crystal and a pump wavelength of 1064 nm is applied at a pulse repetition rate of 300 kHz. For alternating illumination, an electro-optical modulator is used as an optical switch. Therefore, the A-line rate for the functional image is 150 kHz, which is half of the laser repetition rate. To enable fast signal processing and real-time displays, parallel signal processing using a graphics processing unit (GPU) is performed. OR-PAM images of the distribution of blood vessels and gold nanorods in a BALB/c-nude mouse's ear can be simultaneously obtained with 500 × 500 pixels and real-time display at 0.49 fps.

Photoacoustic imaging (PAI) is a noninvasive imaging tool to visualize optical absorbing contrast agents. Due to high ultrasonic resolution and superior optical sensitivity, PAI can be used to monitor nanoparticle-mediated cancer therapy. The current study synthesized Food and Drug Administration-approved Prussian blue (PB) in the form of nanoparticles (NPs) with the peak absorption at 712 nm for photoacoustically imaging tumor-bearing mouse models. To monitor PB NPs from the background tissue in vivo, we also developed a new 700-nm-region stimulated Raman scattering (SRS) source (pulse energy up to 200 nJ and repetition rate up to 50 kHz) and implemented optical-resolution photoacoustic microscopy (OR-PAM). The SRS-assisted OR-PAM system was able to monitor PB NPs in the tumor model with micrometer resolution. Due to strong light absorption at 712 nm, the developed SRS light yielded a two-fold higher contrast from PB NPs, in comparison with a 532-nm pumping source. The proposed laser source involved cost-effective and simple system implementation along with high compatibility with the fiber-based OR-PAM system. The study highlights the OR-PAM system in conjunction with the tunable-color SRS light source as a feasible tool to assist NP-mediated cancer therapy.

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Abstract:We demonstrate a novel electro-optic swept source for wavenumber-linear interferometric sensing and imaging applications. The electro-optic swept source based on an acousto-optic tunable filter (AOTF) provides high environmental stability and arbitrary drive function sweeping because the electro-optic wavelength selection does not depend on a mechanical moving component to tune the output lasing wavelength. We show improved stability of the suggested electro-optic swept source, compared to a conventional swept source based on a fiber Fabry-Perot tunable filter (FFP-TF). Various types of wavelength sweeping are demonstrated by applying the programmed drive function to the applied radio frequency (RF) of the AOTF. We demonstrated improved image quality of optical coherence tomography (OCT) by using the wavenumber-linear drive function of a simple triangular signal, which has a high wavenumber-linearity with an R-square value of 0.99991.

Paintings have high cultural and commercial value, so that needs to be preserved. Many techniques have been attempted to analyze properties of paintings, including X-ray analysis and optical coherence tomography (OCT) methods, and enable conservation of paintings from forgeries. In this paper, we suggest a simple and accurate optical analysis system to protect them from counterfeit which is comprised of fiber optics reflectance spectroscopy (FORS) and line laser-based topographic analysis. The system is designed to fully cover the whole area of paintings regardless of its size for the accurate analysis. For additional assessments, a line laser-based high resolved OCT was utilized. Some forgeries were created by the experts from the three different styles of genuine paintings for the experiments. After measuring surface properties of paintings, we could observe the results from the genuine works and the forgeries have the distinctive characteristics. The forgeries could be distinguished maximally 76.5% with obtained RGB spectra by FORS and 100% by topographic analysis. Through the several executions, the reliability of the system was confirmed. We could verify that the measurement system is worthwhile for the conservation of the valuable paintings. To store the surface information of the paintings in micron scale, we created a numerical database. Consequently, we secured the databases of three different famous Korean paintings for accurate authenticity.