Ultra-widefield photoacoustic microscopy with a dual-channel slider-crank laser-scanning apparatus for in vivo biomedical study

Nguyen, Van Tu; Truong, Nguyen Thanh Phong; Pham, Van Hiep; Choi, Jaeyeop; Park, Sumin; Ly, Cao Duong; Cho, Soon-Woo; Lim, Hae Gyun; Kim, Chang-Seok; Oh, Junghwan

doi:10.1016/j.pacs.2021.100274

Cited by 11 publications

(9 citation statements)

References 41 publications

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“…The choice of laser source depends on the specific application and the desired imaging depth. Additionally, photoacoustic imaging can be performed in various modes, including linear and nonlinear modes, depending on the desired imaging resolution and contrast [11] . There have been several recent advances in photoacoustic biomedical imaging that have the potential to enhance the capabilities and applications of this technique.…”

Section: Acoustic Wave Generationmentioning

confidence: 99%

“…The technique works by illuminating tissue with a pulsed laser, which causes the tissue to heat up and expand, generating an acoustic wave that can be detected and used to create images. 2021) reported Ultra-widefield photoacoustic microscopy with a dual-channel slidercrank laser-scanning apparatus for in vivo biomedical study [11] . The developed PAM system achieves an ultra-widefield scanning area of 24 mm and a high B-scan speed of 32 Hz, providing stable imaging.…”

Section: Introductionmentioning

confidence: 99%

“…By using different wavelengths of light to excite different types of tissue, it is possible to create images that reveal the distribution of oxygenated and deoxygenated blood, as well as the flow of blood through vessels [14] . [11] . (ii) A framework for segmenting skin and blood vessel profiles in vivo photoacoustic images using deep learning.…”

Section: Introductionmentioning

confidence: 99%

“…(C) The 3D image of the mouse ear. (reprinted with permission from Nguyen et al Elsevier, 2021)[11] . (ii) A framework for segmenting skin and blood vessel profiles in vivo…”

mentioning

confidence: 99%

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Photoacoustic Imaging an Emerging Technique for Biomedical Imaging

Park

Choi

2023

BME Horizon

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Photoacoustic imaging (PAI) is a rapidly growing biomedical imaging technique that combines the advantages of optical and acoustic imaging. This technique utilizes pulsed laser light to generate acoustic waves in biological tissue, which are then detected and used to create high-resolution images of tissue structure and function. PAI has several advantages over other imaging modalities, including its ability to provide functional information about tissue properties such as blood oxygenation and blood flow, as well as its ability to image tissue at greater depths than many other techniques. However, there are also challenges associated with PAI, including the need for specialized equipment and concerns regarding the potential for tissue damage due to laser exposure. Despite these challenges, PAI is a promising technique with many potential applications in biomedical research and clinical practice, and ongoing research in this area is likely to yield further advances in the coming years.

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Section: Acoustic Wave Generationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…(C) The 3D image of the mouse ear. (reprinted with permission from Nguyen et al Elsevier, 2021)[11] . (ii) A framework for segmenting skin and blood vessel profiles in vivo…”

mentioning

confidence: 99%

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Photoacoustic Imaging an Emerging Technique for Biomedical Imaging

Park

Choi

2023

BME Horizon

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“… – 22 Among the currently available systems for PA imaging, PA microscopy (PAM) yields high-resolution images with a resolution of a few micrometers by tightly focusing light and/or acoustic waves on a single spot 23 – 36 Furthermore, the PAMs have achieved high-speed capability due to the introduction of micro-electro-mechanical system (MEMS)-based mirror scanning, 37 and since then, various high-speed water-immersible scanners such as galvanometers and polygon-mirror scanners have been developed to increase the imaging speed of PAMs 38 , 39 . Notably, this imaging technique has been used to visualize and monitor microvessels in various studies focusing on wound healing, stimulus response, drug delivery, and regenerative medicines 40 …”

Section: Introductionmentioning

confidence: 99%

In vivo quantitative photoacoustic monitoring of corticosteroid-induced vasoconstriction

et al. 2023

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Significance: Corticosteroids-commonly prescribed medications for skin diseases-inhibit the secretion of vasodilators, such as prostaglandin, thereby exerting anti-inflammatory action by constricting capillaries in the dermis. The effectiveness of corticosteroids is determined by the degree of vasoconstriction followed by skin whitening, namely, the blanching effect. However, the current method of observing the blanching effect indirectly evaluates the effects of corticosteroids.Aim: In this study, we employed optical-resolution photoacoustic (PA) microscopy (OR-PAM) to directly visualize the blood vessels and quantitatively evaluate vasoconstriction.Approach: Using OR-PAM, the vascular density in mice skin was monitored for 60 min after performing each experimental procedure for four groups, and the vasoconstriction was quantified. Volumetric PA data were segmented into the papillary dermis, reticular dermis, and hypodermis based on the vascular characteristics obtained through OR-PAM. The vasoconstrictive effect of each skin layer was quantified according to the dermatological treatment method.Results: In the case of corticosteroid topical application, vasoconstriction was observed in the papillary (56.4 AE 10.9%) and reticular (45.1 AE 4.71%) dermis. For corticosteroid subcutaneous injection, constriction was observed solely in the reticular (49.5 AE 9.35%) dermis. In contrast, no vasoconstrictions were observed with nonsteroidal topical application. Conclusions:Our results indicate that OR-PAM can quantitatively monitor the vasoconstriction induced by corticosteroids, thereby validating OR-PAMs potential as a practical evaluation tool for predicting the effectiveness of corticosteroids in dermatology.

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