Background: Platelet-rich fibrin (PRF) is a biomaterial whose frame is a network of fibrin fibers containing cells from peripheral blood and growth factors. PRF biomaterials have great potential for application in regenerative medicine, especially in maxillofacial surgery and orthopedic trauma. The purpose of this study is to investigate the cytological and histological properties of PRF in order to provide the background knowledge for the applied studies of PRF. Materials and Methods: Peripheral blood from 10 rabbits collected via the ear artery was used to generate platelet-rich plasma and platelet-rich fibrin. Blood samples with anticoagulant are centrifuged to obtain fractions of platelet-rich plasma (PRP) and Platelet rich and white blood cell rich plasma (L-PRP). Plasma is fibrinated with Calcium chloride (CaCl2) to produce platelet-rich fibrin (PRF), Platelet rich and white blood cell rich fibrin (L-PRF). The cellular composition of PRP and L-PRP was investigated using an automated veterinary hematology analyzer. The histological structures of PRF and L-PRF were examined by Hematoxylin-Eosin and Sirius Red staining. Results: The results of cell composition showed that PRP almost did not contain cellular components such as white blood cells and red blood cells, and the platelet concentration was lower than that of peripheral blood. Meanwhile, L-PRP contains a large number of cellular components, especially a high density of white blood cells and platelets. Histological examination results showed that PRF had a dense network of fibrin fibers and lacked the presence of cells. Conclusions: The PRF and L-PRF biomaterials have a suitable spatial structure as a natural biological substrate. Furthermore, L-PRF has a rich cellular component that has great potential in promoting tissue regeneration Key words: Biomaterials, Platelet-rich plasma (PRP), platelet rich and white blood cell rich plasma (L-PRP), tissue regenerative medicine, histological structure
Background: The gastrointestinal tract is the most common site of extranodal Non-Hodgkin lymphoma, accounting for 20% to 40% of all extranodal lymphomas. This study aims to accomplish the objective: classify the prevalent histopathological patterns of gastrointestinal lymphoma according to WHO 2019 classification based on histopathology and immunohistochemistry. Subjects and methods: A cross-sectional study sampled on 76 gastrointestinal lymphoma patients to be examined and treated at The Hue University of Medicine and Pharmacy Hospital and Viet Nam National Cancer Hospital from January 2021 to June 2022. Results: B cell and T cell Lymphoma made up 97.4% and 2.6%, respectively; among them, the most common pattern was diffuse large B-cell lymphoma (59.2%,), Mucosa-associated lymphoid tissue lymphoma (17.1%), Mantle cell lymphoma (15.8%), Follicular lymphoma (2.6%), High-grade B-cell lymphoma (1.3%), and Small lymphocytic lymphoma (1.3%). The most frequent position was the stomach (60.5%), the rectum and colon, small intestine, and ileocecal region at 19.7%, 10.5%, and 9.3%, respectively. Conclusions: Using histopathology and immunohistochemistry can classify the majority of gastrointestinal Non - Hodgkin lymphoma according to the WHO 2019 classification. Immunohistochemistry plays an important role in the classification of gastrointestinal lymphomas. Key words: gastrointestinal lymphoma, WHO 2019 classification, immunohistochemistry
Background: Lung cancer is the most common cancer and one of the leading causes of cancer-related deaths worldwide. In Vietnam, there have been many research associated with lung cancer, focusing mainly on the aspects of epidemiology, histopathological diagnosis and treatment therapy. The research of the expression of immunohistochemical markers to determine the immunophenotype of tumor cells in lung cancer and their relationship to histopathology and stage of the disease has not been systematically studied. Objectives: (1) To determine the expression of IHC markers in lung cancer, histopathology classification during immunohistochemistry evaluation, (2) to investigate the supplementary values of immunohistochemical test and immunophenotypes of common types of lung cancer. Materials and methods: 104 samples of patients who came for lung cancer examination and treatment at Hue Central Hospital and Hue University of Medicine and Pharmacy Hospital from October 2019 to July 2021, using cross-sectional descriptive study methods. Results: Histopathologically, the most common types were adenocarcinoma 36.5%, followed by squamous cell carcinoma (34.6%), small cell carcinoma (17.3%), and squamous cell carcinoma (17.3%), large cells carcinoma (2.9%), others (8.7%). after Immunohistochemical staining indicates that the most popular types were adenocarcinoma (48.1%), squamous cell carcinoma (24.0%), small cell carcinoma (15.4%), large cell carcinoma (3.8%), adeno-squamous cell carcinoma (1.0%) and other types (7.7%). Conclusions: Immunohistochemistry helps to determine the immunophenotype, which will contribute to the routine histopathological diagnosis of HE staining of lung cancer types, supporting targeted treatment for patients. Keywords: Lung cancer, Histopathology, Immunohistochemistry, Immunophenotype
Gastrointestinal lymphoma has a better prognosis than primary adenocarcinoma and responds well to chemotherapy if diagnosed correctly and promptly. WHO classification (2016) for this heterogeneous group of diseases has emphasized the role of immunohistochemistry and molecular biology. Determining the origin of tumor cells as B-lymphocytes or T-lymphocytes, in the marginal zone, mantle zone, or germinal center regions by identifying specific immune phenotypes by immunohistochemical techniques is an advanced method. Thereby helping to identify most types of gastrointestinal lymphoma, helping to orient targeted treatment, and contributing to improving prognosis as well as improving quality of life for patients. The role of molecular biology in the classification and prognosis of subtypes of gastrointestinal lymphoma is still studying. Key words: Gastrointestinal lymphoma classification, Immunohistochemistry, Review
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