<em>Malaria is one of the public health problems that can cause death in infants, children under five, pregnant women and reduces work productivity. Vector control by chemical means leads to vector resistance and environmental pollution and negatively impacts public and animal health. This study aims to determine the effectiveness of the bark extract of Maja (Aegle marmelos L) against the death of Anopheles sp mosquito larvae with concentrations of 5%, 10%, 20%, 40%, and 50% and the observation of contact times every 15 minutes, 30 minutes, 60 minutes, 90 minutes and 120 minutes. The study used a Completely Randomized Design (CRD) with Factorial. Extraction used maceration method, with 70% ethanol as solvent. Larvae of Anopheles sp. instar III obtained from malaria endemic area. The test used a 200 ml container, with two replications. All data were analyzed by two way ANOVA and Tukey HSD test. This study obtained mortality of 93.3% larvae at a concentration of 50% and a contact time of 120 minutes. The results of statistical analysis showed a significant effect of concentration (p=0.0001), contact time (p=0.0001), and interaction concentration-contact time (p=0.004) on the mortality of Anopheles sp. mosquito larvae. This study has proven the use of bark extract of Maja (Aegle marmelos L) as an alternative bio-insecticide in controlling mosquito larvae of Anopheles sp.</em>
Hazardous and Toxic Material Waste is the residue of a business and service facility activity containing B3. Hazardous and toxic waste (B3) at the Puskesmas has excellent potential to pollute the environment, cause accidents, and endanger public health due to viruses and bacteria that are the source of disease transmission; for this reason, this research is essential to do as an illustration of B3 waste management which has not been optimal at Health Centre in Bandar Lampung which has never been done before. The results of the research on the B3 solid waste management process at the Bandar Lampung City Health Center facilities with the same standards did not meet the technical requirements and the utilization of the facilities; the process of transporting Puskesmas waste was transported entirely to RSUD Dr. A. Dadi Tjokrodipo Bandar Lampung City. A destruction company with a B3 waste processing operational legality permit carries out the processing, burial, and stockpiling operations. Recommendations are needed to provide policies for the Forestry Environment Service and Bandar Lampung City Health Office related to solid medical waste management with the fulfillment of waste management facilities/infrastructure and the completion of an incinerator with permission from PT. Wastec International, PT. Tenang Jaya Sejahtera, and PT. Pengolahan Limbah Industri Bekasi . In addition, it is necessary to supervise and monitor the processing of Puskesmas waste in the destruction company.
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