In situ plasma membrane H(+)-ATPase activity was monitored during pH-regulated dimorphism of Candida albicans using permeabilized cells. ATPase activity was found to increase in both the bud and germ tube forming populations at 135 min which coincides with the time of evagination. Upon reaching the terminal phenotype the mycelial form exhibited higher H(+)-ATPase activity as compared to the yeast form. At the time of evagination H(+)-efflux exhibited an increase. K+ depletion resulted in attenuated ATPase activity and glucose induced H(+)-efflux. The results demonstrate that ATPase may play a regulatory role in dimorphism of C. albicans and K+ acts as a modulator.
Amino acid uptake was followed during pH‐regulated dimorphism of Candida albicans. It was observed that transport activities of various amino acids differed with the morphological phenotype. The uptake rates of l‐alanine, l‐phenylalanine and of l‐lysine were lower and those of l‐methionine were higher in elongated hypha (germ tube), while the rates of glycine, l‐glutamic acid and l‐proline were similar in bud and hyphal phenotypes. Minimum threshold of amino acids transport activity is required at the time of phenotypic commitment in a diverging population of Candida albicans.
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