Lipids as modulators of ethanol tolerance in yeast

Mishra, Prashant; Kaur, Simminder

doi:10.1007/bf00169336

Cited by 52 publications

(23 citation statements)

References 53 publications

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“…The ergosterol content of ICV K1 (V-1116) never exceeded 5 mol% and was relatively consistent throughout fermentation. It has previously been reported that elevated ergosterol levels are associated with ethanol tolerance in yeast (42,43,51); however, studies involving wine yeast have reported that elevated levels of ergosterol were not critical to the ability to tolerate increasing levels of self-produced ethanol (45,46,52), an observation that was corroborated with this strain and others studied here.…”

Section: Discussionsupporting

confidence: 89%

“…In yeast, a number of studies investigating how lipid composition contributes to alcohol tolerance have concluded that elevated levels of ergosterol are important in mitigating the toxic effects of ethanol (42,43,51). However, other studies investigating wine fermentation yeast strains have found that ergosterol levels do not significantly contribute to ethanol tolerance (45,46,52).…”

Section: Discussionmentioning

confidence: 99%

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Ethanol Production and Maximum Cell Growth Are Highly Correlated with Membrane Lipid Composition during Fermentation as Determined by Lipidomic Analysis of 22 Saccharomyces cerevisiae Strains

Henderson

Lozada-Contreras

Jiranek

et al. 2013

Appl Environ Microbiol

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dOptimizing ethanol yield during fermentation is important for efficient production of fuel alcohol, as well as wine and other alcoholic beverages. However, increasing ethanol concentrations during fermentation can create problems that result in arrested or sluggish sugar-to-ethanol conversion. The fundamental cellular basis for these problem fermentations, however, is not well understood. Small-scale fermentations were performed in a synthetic grape must using 22 industrial Saccharomyces cerevisiae strains (

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Ethanol Production and Maximum Cell Growth Are Highly Correlated with Membrane Lipid Composition during Fermentation as Determined by Lipidomic Analysis of 22 Saccharomyces cerevisiae Strains

Henderson

Lozada-Contreras

Jiranek

et al. 2013

Appl Environ Microbiol

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“…Recently, there has been some progress in understanding ethanol tolerance. Plasma membrane phospholipids have been shown to play an important role in the ethanol tolerance mechanism (D'amore & Stewart, 1987;Mishra & Kaur, 1991;Mizoguchi & Hara, 1996). The difference in fatty acid composition of plasma membranes is shown to dramatically alter the permeability barrier of plasma membranes.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Measurement of Yeast Cell Density and Vitality Using 2,3,5-Trimethyl-1,4-benzoquinone and Effect of Ethanol on the Metabolism of Quinone by Yeast Cells

Tsukatani

Ide

Oba

et al. 2003

FSTR

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“…Another opportunity for a cell to overcome toxic effects of lipophilic compounds is altering the fatty acid composition of the membrane (6,15). This could lead to different sensitivities, probably as a result of altered partition characteristics of the lipophilic compounds (6).…”

Section: Methodsmentioning

confidence: 99%

Effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes

et al. 1992

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Tetralin is toxic to bacterial cells at concentrations below 100 ,mollliter. To assess the inhibitory action of tetralin on bacterial membranes, a membrane model system, consisting of proteoliposomes in which beef heart cytochrome c oxidase was reconstituted as the proton motive force-generating mechanism, and several gram-positive and gram-negative bacteria were studied. Because of its hydrophobicity, tetralin partitioned into lipid membranes preferentially (lipid/buffer partition coefficient of tetralin is approximately 1,100). The excessive accumulation of tetralin caused expansion of the membrane and impairment of different membrane functions. Studies with proteoliposomes and intact cells indicated that tetralin makes the membrane permeable for ions (protons) and inhibits the respiratory enzymes, which leads to a partial dissipation of the pH gradient and electrical potential. The effect of tetralin on the components of the proton motive force as well as disruption of protein-lipid interaction(s) could lead to impairment of various metabolic functions and to low growth rates. The data offer an explanation for the difficulty in isolating and cultivating microorganisms in media containing tetralin or other lipophilic compounds.Interest in the application of water-immiscible organic compounds in fermentations has increased in the last decade. Many lipophilic compounds are harmful to microorganisms, impair growth, and even inhibit other biological reactions. Knowledge of the toxic action of lipophilic compounds on bacterial cells is mainly restricted to the relation between the hydrophobicity (13) of a compound and its effect on a specific enzyme (19). In most studies, the cytoplasmic membrane is mentioned as a possible target, but information about the nature of the toxic action is not presented (1,19).A correlation between the hydrophobicity of a compound and its effects on cells was first observed for anesthetics (27)(28)(29), which provided a basis for calculating a dose-effect relationship. In addition, the uncoupling effects of lipophilic compounds on energy transduction have been studied in animal cells (23). For microorganisms, only a few studies on the toxic effects of lipophilic compounds on various membrane functions have been performed (3,32,33). These studies showed that hydrocarbons, e.g., 3-pinene (32) and cyclohexane (33), impaired energy transduction in both mitochondrial and plasma membranes of yeast cells. Studies on the toxic effects of ethanol on yeast cells indicated that the Ap across the plasma membrane was dissipated in the presence of ethanol (3), probably due to an increased influx of protons (3,12).In this investigation, the toxic action of the lipophilic compound tetralin on bacteria was studied. Tetralin was found to be toxic to bacterial cells at concentrations below 100 ,umol/liter (30). Tetralin, 1,2,3,4-tetrahydronaphthalene, is a bicyclic molecule that consists of an aromatic and an alicyclic moiety. The compound is widely applied as an industrial solvent and as a substitute f...

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Lipids as modulators of ethanol tolerance in yeast

Cited by 52 publications

References 53 publications

Ethanol Production and Maximum Cell Growth Are Highly Correlated with Membrane Lipid Composition during Fermentation as Determined by Lipidomic Analysis of 22 Saccharomyces cerevisiae Strains

Ethanol Production and Maximum Cell Growth Are Highly Correlated with Membrane Lipid Composition during Fermentation as Determined by Lipidomic Analysis of 22 Saccharomyces cerevisiae Strains

Electrochemical Measurement of Yeast Cell Density and Vitality Using 2,3,5-Trimethyl-1,4-benzoquinone and Effect of Ethanol on the Metabolism of Quinone by Yeast Cells

Effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes

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