Dimorphism-associated changes in intracellular pH of Candida albicans

Kaur, Simminder; Mishra, Prashant; Prasad, Rajendra

doi:10.1016/0167-4889(88)90202-9

Cited by 36 publications

(26 citation statements)

References 32 publications

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“…The pHi was measured as described earlier (Kaur et al 1988h) to form mycelia at pH 6.5 showed only a slight increase in pHi (0.12 units) at about the time of germ-tube emergence. This is in agreement with our earlier observation in C. alhica~zs ATCC 10261 that pHi increased by 0.44 and 0.14 pH units in populations destined to form buds and mycelia, respectively (Kaur et al 19886). However, these results are contrary to earlier observations in which cells were allowed to differentiate under zinc-sufficient conditions (Stewart et al 1988(Stewart et al , 1989.…”

Section: Resultssupporting

confidence: 90%

Differential increase in cytoplasmic pH at bud and germ tube formation in Candida albicans: studies of a nongerminative variant

Kaur¹,

Mishra²

1994

Can. J. Microbiol.

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The changes in cytoplasmic pH (pHi) accompanying the morphological transition of Candida albicans were studied using a nongerminative variant. A transient cytoplasmic alkalinization at the time of evagination was observed in both the variant and its parent. Under zinc-deficient conditions the wild-type cells that formed buds at pH 4.5 and mycelia at pH 6.5 showed an increase in pHi of 0.58 and 0.12 pH units, respectively. Under similar conditions the pHi of the nongerminative variant that formed buds at both pH 4.5 and 6.5 increased by 0.66 and 0.40 pH units, respectively, suggesting that a greater magnitude of increase in pHi at the time of evagination is probably needed for bud formation. These results provide evidence for a correlation between intracellular alkalinization and time of cell differentiation in C. albicans.

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Section: Resultssupporting

confidence: 90%

Differential increase in cytoplasmic pH at bud and germ tube formation in Candida albicans: studies of a nongerminative variant

Kaur¹,

Mishra²

1994

Can. J. Microbiol.

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“…It therefore follows that cellular control mechanisms for pHi which include activities of the plasma membrane-and vacuolar H+ -ATPases, are also linked to cellular responses to external stimuli. In C. albicans, an organism where external pH can be used to control dimorphic transitions (Chapter 19), cytoplasmic alkalinization accompanies germ tube formation, pHi rising from pH 6.8 to pH 7.5 within 2 min and to > pH 8.0 after 20 min (Kaur et al, 1988;Stewart et ai., 1988). Non-dimorphic variants grown under germ tubeinducing conditions, or cells grown in conditions that do not induce germ tube formation, do not exhibit such cytoplasmic alkalinization (Stewart et al, 1988(Stewart et al, , 1989.…”

Section: Intracellular Phmentioning

confidence: 99%

Signal Transduction In Fungi

Gadd

1995

The Growing Fungus

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“…Intracellular pH (pHi) was measured following the methods of Kaur et al, 1988 andManzoor et al, 2002, with minor modifications. Mid-exponential-phase cells grown in YPD medium were harvested and washed twice with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Influences of cinnamic aldehydes on H+ extrusion activity and ultrastructure of Candida

Shreaz

Bhatia

Khan

et al. 2013

Journal of Medical Microbiology

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The antifungal effects of cinnamaldehyde, 4-hydroxy-3-methoxycinnamaldehyde (coniferyl aldehyde) and 3,5-dimethoxy-4-hydroxycinnamaldehyde (sinapaldehyde) were investigated against 65 strains of Candida (six standard, 39 fluconazole-sensitive and 20 fluconazoleresistant). MICs of cinnamaldehyde, coniferyl aldehyde and sinapaldehyde ranged from 100 to 500 mg ml "1 , 100 to 300 mg ml "1 and 100 to 200 mg ml "1 , respectively. All tested isolates showed a marked sensitivity towards these aldehydes in spot and time-kill assays. Sinapaldehyde was found to be the most effective, followed by coniferyl aldehyde and cinnamaldehyde. At their respective MIC 90 values, the three compounds caused mean inhibition levels of glucosestimulated H + -efflux of 36, 34 and 41 % (cinnamaldehyde), 41, 42 and 47 % (coniferyl aldehyde) and 43, 45 and 51 % (sinapaldehyde) for standard-sensitive, clinical-sensitive and clinicalresistant isolates, respectively. Inhibition levels of H + -efflux caused by plasma membrane ATPase inhibitors N,N9-dicyclohexylcarbodiimide (100 mM) and diethylstilbestrol (10 mM) were 34, 45 and 44 %, and 57, 39 and 35 %, for standard-sensitive, clinical-sensitive and clinical-resistant isolates, respectively. Intracellular pH (pHi) was found to decrease by 0.34, 0.42 and 0.50 units following incubation with three tested aldehydes from the control pHi of 6.70. Scanning electron microscopy and transmission electron microscopy analysis was performed on a representative strain, C. albicans 10261, showing alterations in morphology, cell wall, plasma membrane damage and lysis. Haemolytic activity of the three compounds varied from 10 to 15 % at their highest MIC compared to an activity level of 20 % shown by fluconazole at 30 mg ml "1 . In conclusion, this study shows significant activity of cinnamic aldehydes against Candida, including azole-resistant strains, suggesting that these molecules can be developed as antifungals.

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Dimorphism-associated changes in intracellular pH of Candida albicans

Cited by 36 publications

References 32 publications

Differential increase in cytoplasmic pH at bud and germ tube formation in Candida albicans: studies of a nongerminative variant

Differential increase in cytoplasmic pH at bud and germ tube formation in Candida albicans: studies of a nongerminative variant

Signal Transduction In Fungi

Influences of cinnamic aldehydes on H+ extrusion activity and ultrastructure of Candida

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