Vitamin D receptor (VDR) has been proposed as a possible marker for fibromyalgia syndrome (FMS). The purpose of this study is to characterize the expression pattern of BsmI polymorphism (rs1544410) in the VDR gene in women with FMS and the genotype-phenotype association.
MethodsA total of 105 FMS patients and 105 controls were included in this study. VDR gene BsmI polymorphism was assessed by polymerase chain reaction (PCR) and restriction fragment length polymerase (RFLP) method.
ResultsThere was no significant difference in the frequency distribution of both genotypes and alleles for VDR gene BsmI polymorphism between FMS patients and controls (p>0.05). The frequencies of BB, Bb, and bb in the VDR gene BsmI polymorphism were 19%, 43%, and 37% in patients, while in controls were 22.9%, 55.2%, and 21.9%. However, we did not find any significant association between the clinical symptoms of this disease and VDR BsmI genotypes among FMS patients (p>0.05).
ConclusionsThe relationship between the VDR gene BsmI polymorphism and FMS could not be determined in this study. However, further studies with a larger sample size may be required to show a relation between the VDR gene BsmI polymorphism and FMS.
The aim: To determine the clinical and the genetic association of the COMT rs4680 SNP in women with FMS.
Materials and methods: Extracted DNA from peripheral blood samples were utilized as template for the PCR and RFLP analysis.
Results: A significant difference was found in the distribution of the COMT genotype between FMS patients and controls (P<0.05). The frequency of GG, AG, AA genotypes were 12%, 72%, 21% in FMS patients and 32%, 62%, 11% in controls. The clinical features of FMS reveal that FIQR and the severity of pain measured by VAS were significantly associated with the COMT rs4680 SNP (P=0.042; P=0.016). The co-dominant model for GG verse v. AG genotype (P=0.004) and AG v. AA genotype (P=0.002) has shown to be high risk for FMS. An increased risk of FMS in the dominant model for (AG+AA) v. GG genotype (P=0.001) and no significant difference was found between (GG+AG) v. AA genotype (P=0.08) in the recessive model. The result indicated that A allele considerably increase the risk of FMS (P=0.004) in comparison to the G allele.
Conclusions: AA genotype and A allele of the COMT rs4680 SNP were significantly associated with severity in FMS patients and also plays a significant role in the clinical manifestation of this disease.
Background
Several psychiatric disorders have been found associated with serotonin transporter gene polymorphisms. The purpose of this study was to explore the correlation between serotonin gene polymorphism (5-HTTLPR & 5-HTTVNTR) in North Indian Women with Fibromyalgia Syndrome (FMS).
Results
105 FMS patients and 105 controls were enrolled in the study. Polymerase chain reaction (PCR) method was used to analyse the 5-HTTLPR & 5-HTTVNTR gene polymorphism. The psychopathology status of the 105 FMS patients and 105 healthy controls was assessed using the Beck Depression Inventory (BDI) and the Symptom Checklist-90-Revised questionnaires (SCL-90-R). Patients with psychiatric symptoms were excluded from the study. No significant differences were found in 5-HTTVNTR genotypes; 10/10 (OR 0.74, P = 1.000), 10/12 (OR 0.72, P = 0.4691) and 12/12 (OR 0.92, P = 0.3697) or 5-HTTLPR genotypes; L/L (OR 0.59, P = 0.7212), S/L (OR 1.10, P = 0.6700) and S/S (OR 0.92, P = 0.8890) between patients and controls.
Conclusion
Hence, we conclude that serotonin gene polymorphism (5-HTTLPR & 5-HTTVNTR) is not associated with FMS in north Indian women.
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