Ecological intensification, or the improvement of crop yield through enhancement of biodiversity, may be a sustainable pathway toward greater food supplies. Such sustainable increases may be especially important for the 2 billion people reliant on small farms, many of which are undernourished, yet we know little about the efficacy of this approach. Using a coordinated protocol across regions and crops, we quantify to what degree enhancing pollinator density and richness can improve yields on 344 fields from 33 pollinator-dependent crop systems in small and large farms from Africa, Asia, and Latin America. For fields less than 2 hectares, we found that yield gaps could be closed by a median of 24% through higher flower-visitor density. For larger fields, such benefits only occurred at high flower-visitor richness. Worldwide, our study demonstrates that ecological intensification can create synchronous biodiversity and yield outcomes.

Alternative pollinators can ensure pollination services if the availability of the managed or most common pollinator is compromised. In this study, the behavior and pollination efficiency of Apis mellifera L. and two species of stingless bees, Plebeia emerina Friese and Tetragonisca fiebrigi Schwarz, were evaluated and compared in flowers of Brassica napus L. 'Hyola 61'. A. mellifera was an efficient pollinator when collecting nectar because it effectively touched the reproductive organs of the flower. In contrast, stingless bees were efficient pollinators only when collecting pollen. The number of pollen grains deposited on the stigma after a single visit by worker bees of the three species was greater than the number of grains resulting from pollination without the bee visits. On average, the three species deposited enough pollen grains to fertilize all of the flower ovules. A. mellifera and P. emerina had similar pollination efficiency because no significant differences were observed in the characteristics of the siliques produced. Although T. fiebrigi is also an effective pollinator, the seed mass produced by their pollination was lower. Native bees promoted similar rates of fruit set compared with A. mellifera. Thus, P. emerina has potential to be used for pollination in canola crops.

A escolha das cultivares possui importância relevante para alcançar o sucesso no cultivo do morangueiro. No entanto, as pesquisas com cultivares, normalmente ocorrem em sistemas convencionais de produção e não em sistemas de cultivo sem solo. Em função disso, avaliaram-se quatro cultivares de morangueiro em sistema sem solo, em casas de vegetação, em duas localidades, com condições ecoclimáticas diferentes, em Eldorado do Sul (Depressão Central) e Caxias do Sul (Serra do Nordeste), no RS. Foram estudadas as cultivares de morangueiro Aromas, Camarosa, Oso Grande e Tudla. O plantio ocorreu em maio de 2005, sendo as mudas colocadas em sacolas que continham substrato composto de 50% de turfa e 50% de casca de arroz queimada. A adubação foi realizada via fertirrigação. A colheita foi feita quando os frutos apresentavam 70% de cor avermelhada até totalmente vermelhos. Os resultados mostraram que o morango, em cultivo sem solo, embora apresentasse menor rendimento por planta que em cultivo tradicional, propiciou melhor aproveitamento interno da casa de vegetação, com reflexos positivos no rendimento por área, em função da alta densidade de plantas e maior facilidade de manejo da cultura. A cultura do morangueiro apresentou maior produtividade no cultivo realizado em Caxias do Sul (235,8 g planta-1), quando comparado a Eldorado do Sul (196,4 g planta-1) mesmo sem interação entre locais e cultivares. Em Caxias do Sul, as cultivares Aromas, Oso Grande e Camarosa apresentaram maiores produtividades quando comparadas a Tudla, mas nenhuma delas apresentou diferença em Eldorado do Sul.

Brassica napus Linnaeus is considered a self-compatible crop; however, studies show that bee foraging elevates their seed production. Considering bee food shortages during the winter season and that the canola is a winter crop, this study aimed to evaluate the foraging behaviour of Apis mellifera Linnaeus, 1758 regarding those flowers, and to verify if it presents adequate behaviour for successfully pollinating this crop in Rio Grande do Sul State. The study was carried out in a canola field, in Southern Brazil. The anthesis stages were morphologically characterised and then related to stigma receptivity and pollen grain viability. Similarly, the behaviour of A. mellifera individuals on flowers was followed, considering the number of flowers visited per plant, the amount of time spent on the flowers, touched structures, and collected resources. Floral fidelity was inferred by analysing the pollen load of bees collected on flowers. The bees visited from 1-7 flowers/plant (x = 2.02; sd = 1.16), the time spent on the flowers varied between 1-43 seconds (x = 3.29; sd = 2.36) and, when seeking nectar and pollen, they invariably touched anthers and stigmas. The pollen load presented 100% of B. napus pollen. The bees' attendance to a small number of flowers/plants, their short permanence on flowers, their contact with anthers and stigma and the integral floral constancy allows their consideration as potential B. napus pollinators.Keywords: behavior, canola, honey bees, anthesis, pollination.Apis mellifera (Hymenoptera: Apidae) como potencial polinizador de Brassica napus (cv. Hyola 432) (Brassicaceae), no Sul do Brasil ResumoBrassica napus Linnaeus é considerada uma cultura autocompatível, entretanto, estudos indicam que o forrageio de abelhas eleva sua produtividade de sementes. Considerando-se a escassez de alimento para abelhas no inverno e a canola sendo uma cultura desse período, objetivou-se avaliar o comportamento de forrageio de Apis mellifera Linnaeus, 1758 nas suas flores e verificar se apresenta comportamento propício ao sucesso de polinização dessa cultura no Rio Grande do Sul. O estudo foi desenvolvido em lavoura de canola, no Sul do Brasil. Os estágios da antese foram caracterizados morfologicamente e relacionados à receptividade do estigma e à viabilidade dos grãos de pólen. Paralelamente, o comportamento de indivíduos de A. mellifera nas flores foi acompanhado, considerando-se o número de flores visitadas por planta, o tempo de permanência nas flores, as estruturas tocadas e os recursos coletados. A fidelidade floral foi inferida analisando-se a carga polínica de abelhas coletadas em flores. As abelhas visitaram entre 1-7 flores/planta (x = 2,02; sd = 1,16), o tempo de permanência nas flores variou entre 1-43 segundos (x = 3,29; sd = 2,36) e, ao buscarem néctar e pólen, invariavelmente tocavam anteras e estigmas. A carga polínica apresentou 100% de pólen de B. napus. A visita a poucas flores/planta, a curta permanência sobre as flores, o contato das abelhas com anteras e estigma e a plena constâ...

Canola crop productivity is benefited by bee pollination and it has been shown that bee communities can be affected by landscape composition. The aim of this study was to analyse the bee community and its relationship to canola seed production in agricultural areas. The density, abundance and richness of floral visitors of Brassica napus cultivar Hyola 61 in six commercial fields in southern Brazil were studied, and their relationships with seed production and the ratio of semi-natural, forested and agricultural areas surrounding the crops were examined. It was observed that canola fields of southern Brazil are surrounded by a homogeneous landscape dominated by agricultural areas. The survey of bees detected a low abundance and richness of native bees in contrast to the high abundance of Apis mellifera. Except for a negative correlation between the abundance of honey bees and the proportion of forested areas within a 2000 m radius from the field (R = -0.90; P = 0.012), no other correlations were found among bee abundance and richness and landscape composition. Although there was not a relationship between A. mellifera and seed set, there was a positive correlation between insect density and seed weight per plant (R = 0.87; P = 0.024). As honey bees were the most captured insect (79%), much of the pollination in this system was probably achieved by honey bees.

Planning the artificial pollination of agricultural crops requires knowledge of the floral biology and reproductive system of the crop in question. Many studies have shown that rapeseed (Brassica napus Linnaeus) is self-compatible and self-pollinated, but its productivity may be increased by insect visitation. In the present study, the floral biology and the response of productivity to insect visitation of two rapeseed cultivars (Hyola 420 and Hyola 61) were analyzed and compared in three regions of Rio Grande do Sul, Brazil. The rapeseed flowers presented three stages during anthesis, with the time periods varying between the cultivars. Both cultivars are self-compatible, but free visitation of insects increased productivity by 17% in the Hyola 420 cultivar and by approximately 30% in the Hyola 61 cultivar. Therefore, it is concluded that the cultivar Hyola 61 is more dependent on insect pollination than Hyola 420.Keywords: floral biology, reproductive system, pollination, rapeseed, Brassica napus. Estudo comparativo da biologia floral e da resposta da produtividade à visita por insetos em duas cultivares de canola (Brassica napus L.) no Rio Grande do SulResumo O planejamento de polinização dirigida nas culturas agrícolas requer o conhecimento da biologia floral e do sistema reprodutivo da cultura em questão. Muitos estudos mostram que a canola (Brassica napus Linnaeus) é auto compatível e auto polinizada, no entanto, sua produtividade pode ser aumentada pela visita por insetos. Nesse estudo, a biologia floral e a resposta da produtividade à visita por insetos de duas cultivares de canola (Hyola 420 e Hyola 61) foram analisadas e comparadas em três regiões do Rio Grande do Sul, Brasil. As flores de canola apresentaram três fases durante a antese, havendo variação no período de tempo de acordo com a cultivar. Ambas as cultivares são auto compatíveis, mas a livre visita por insetos aumentou a produtividade em 17% na cultivar Hyola 420 e cerca de 30% na cultivar Hyola 61. Dessa maneira, concluímos que a cultivar Hyola 61 é mais dependente da polinização entomófila que a Hyola 420.Palavras-chave: biologia floral, sistema reprodutivo, polinização, canola, Brassica napus.

To characterize the populational diversity of Mourella caerulea , an endemic stingless bee from the Pampa biome, we collected workers of the stingless bee Mourella caerulea from 24 colonies of five localities in Southern Brazil and analyzed it using geometric morphometrics of forewings, mtDNA cytochrome oxidase I variability, and cuticular hydrocarbon (CHC) chemical analysis. The morphometric analysis discriminated the populations of M. caerulea from different physiographic regions. There was a positive correlation between morphometric and geographic distances. CHC profiles also differentiated the colonies from different localities. We found six particular haplotypes, nucleotide diversity (π) of 0.01631, and a haplotype diversity (Hd) of 0.74. In this sense, the comparison of the population belonging to different physiographic regions indicates that we need to give particular attention to M. caerulea at the moment of creating conservation strategies for South Brazilian Fauna, once it is the only species of this monospecific genus, and its populations are much differentiated from each other. stingless bees / Mourella caerulea / geometric morphometrics / mitochondrial DNA / cuticular hydrocarbons Electronic supplementary material The online version of this article (

Resumo -O objetivo deste trabalho foi avaliar o efeito de diferentes tipos de polinização sobre a qualidade de frutos de cultivares de morangueiro e sua contribuição isolada para a massa dos frutos, bem como determinar o potencial de Plebeia nigriceps (Hymenoptera: Apidae, Meliponini) como agente polinizador em ambiente protegido. As cultivares Aromas, Diamante e Cegnidarem foram submetidas a tratamentos com autopolinização, polinização por P. nigriceps e polinização livre. Os experimentos foram conduzidos em estufa tipo pampeana, coberta com polietileno transparente e desprovida de telas anti-insetos nas laterais, com 1.344 plantas. Para as avaliações, foram marcadas 56 flores primárias em botão, de cada cultivar, e considerou-se cada planta uma repetição. Avaliaram-se massa de matéria fresca, peso, diâmetro, comprimento e presença de deformação nos frutos. A polinização entomófila tem contribuição variada à massa dos frutos, de acordo com a cultivar. As cultivares apresentam sensibilidade variada à autopolinização, no que se refere à incidência de frutos deformados. A interferência da polinização entomófila na produtividade do morangueiro está mais relacionada à redução do percentual de frutos deformados do que ao aumento da massa dos frutos em si. O comportamento de P. nigriceps indica que a espécie apresenta potencial para polinização da cultura do morangueiro em ambiente protegido.Termos para indexação: Fragaria x ananassa, Plebeia nigriceps, autopolinização, produtividade, qualidade de fruto. Performance of strawberry cultivars subjected to different types of pollination in a greenhouseAbstract -The objective of this work was to evaluate the effects of different types of pollination on the fruit quality of strawberry cultivars, and their isolated contribution to fruit mass, as well as to determine the potential of Plebeia nigriceps (Hymenoptera: Apidae, Meliponini) as a pollination agente in protected environment. The cultivars Aromas, Diamante, and Cegnidarem were subjected to self-pollination, pollination by P. nigriceps, and free pollination treatments. The experiments were carried out in a "pampeana" greenhouse, covered with transparent polyethylene and without anti-insect screens on the sides, with 1,344 plants. For the evaluations, 56 primary flowers of each cultivar, still in bud, were marked, and each of these plants was considered a replicate. Fresh matter mass, weight, diameter, and misshape incidence in fruit were evaluated. The contribution of Entomophilous pollination on fruit weight varies according to the cultivar. Cultivars have different sensibility to self-pollination, regarding misshape incidence on fruit. Entomophilous interference on strawberry yield is more related to reduction of misshape incidence than to increases in fruit mass itself. P. nigriceps behavior indicates that this species has good potential for pollinating strawberry crop in protected environment.