The emergence of antimicrobial-resistantfood-borne bacteria is a great challenge to public health. This study was conducted to characterize and determine the resistance profile of Salmonella strains isolated from foods including sesames, ready-to-eat (RTE) salads, mango juices, and lettuce in Burkina Faso. One hundred and forty-eight biochemically identified Salmonella isolates were characterized by molecular amplification of Salmonella marker invA and spiC, misL, orfL, and pipD virulence genes. After that, all confirmed strains were examined for susceptibility to sixteen antimicrobials, and PCR amplifications were used to identify the following resistance genes: blaTEM, temA, temB, StrA, aadA, sul1, sul2, tet(A), and tet(B). One hundred and eight isolates were genetically confirmed as Salmonella spp. Virulence genes were observed in 57.4%, 55.6%, 49.1%, and 38% isolates for pipD, SpiC, misL, and orfL, respectively. Isolates have shown moderate resistance to gentamycin (26.8%), ampicillin (22.2%), cefoxitin (19.4%), and nalidixic acid (18.5%). All isolates were sensitive to six antibiotics, including cefotaxime, ceftazidime, aztreonam, imipenem, meropenem, and ciprofloxacin. Among the 66 isolates resistant to at least one antibiotic, 11 (16.7%) were multidrug resistant. The Multiple Antimicrobial Resistance (MAR) index of Salmonella serovars ranged from 0.06 to 0.53. PCR detected 7 resistance genes (tet(A), tet(B), blaTEM, temB, sul1, sul2, and aadA) in drug-resistant isolates. These findings raise serious concerns because ready-to-eat food in Burkina Faso could serve as a reservoir for spreading antimicrobial resistance genes worldwide.
Background and Aim: The use of pesticides in vegetable production can cause public health problems because these agrochemicals can leave residues in foodstuffs and disrupt the appropriate functioning of the organism. The aim of this study was to assess the level of contamination of lettuce and tomato by pesticide residues. Materials and Methods: This study was conducted from February 12 to May 3, 2021, in concerned five market gardening sites in the city of Ouagadougou. A survey was conducted among 58 market gardeners selected at random from the sites of Boulmiougou, Tanghin No. 1 and 2, Bissigin, and the National School of Public Health. Simultaneously, 25 lettuce samples and 25 tomato samples were collected in a randomized manner for the examination and determination of pesticide residues using the quick, easy, cheap, effective, rugged, and safe method. Results: The market gardening population was predominantly represented by women who had a low level of education. The cultivated species were a mixture of exotic cultures and traditional cultures. These crops were treated with various pesticides, the most common of which belonged to the organochlorine family. The tomato was the crop most contaminated by pesticide residues. However, no active ingredient from the synthetic pyrethroid family was found on lettuce or tomato. Conclusion: Raising the awareness of market gardeners on good practices in the use of pesticides and on alternative methods to synthetic phytosanitary products is of paramount importance for the health of consumers.
Introduction. In the response to the coronavirus pandemic, several PCR kits have been developed with varying performance. Aim. In this study, we compared the results of SARS-CoV-2 testing using the Xpert® Xpress SARS-CoV-2 (Cepheid EUA) Testing with the TaqPathTM -COVID-19 CE IVD kit. Methods. A total of 92 nasopharyngeal swab samples from patients during September to November 2021 at the National Public Health Laboratory in Ouagadougou, Burkina Faso. These samples were analyzed using both the Xpert Xpress SARS-CoV-2 assay and the TaqPath-COVID-19-CE IVD kit on the QuantStudio 5 thermal cycler after extraction with the ANDiS 350 automated extractor. Results. The majority of patients was male 68.48% (63/92) and female 31.52% (29/92). The mean age was 42.2 ± 13.76 years with extremes of 12 and 70 years. The Xpert Xpress SARS-CoV-2 kit showed positive agreement with a kappa coefficient of 93.35% [85.9; 100] compared to the TaqPath-COVID-19-CE IVD kit. It also showed a sensitivity of 100%, a specificity of 92.68 and negative and positive predictive values of 100% and 94.44% respectively. Conclusion. The Xpert Xpress test is a very simple assay that compares favorably with the TaqPath-COVID-19-CE IVD test and can be reliably used for the detection of SARS-CoV-2 in nasopharyngeal specimens.
Knowledge of the risks of chemicals remains low among most parts of the African population. This situation is further complicated by the widespread lack of reliable data and information on the toxicity and safe use of chemicals. The present study was conducted to describe the chemical hazards to which workers in auto body paint shops would be exposed.The cross-sectional and descriptive study was conducted in car body paint shops in the city of Ouagadougou. The data collection consisted of an inventory of the products, direct observation of the workers in the work situation, and interviews.A total of 17 chemical products were inventoried in all the car body paint shops. Of the 17 products inventoried, 15 were flammable solvents (88.23%), 2 (11.77%) were in paste form and 1 (5.88%) was an oxidizer. All the products were dangerous for health and among them 6 (35.29%) were dangerous for the environment according to their pictograms. These products were handled without special precautions.All these products present risks for human health and the environment. In view of the working conditions, there is a potential exposure of the workers to all the chemicals as well as the inhabitants of the nearby residential areas.
Introduction. The use of partially or untreated wastewater in the irrigation of vegetable crops constitutes a risk of microbiological contamination that can cause diarrhea in the consumer. Aim. This study mainly aimed to assess the level of contamination of irrigation water and lettuce as well as to determine the antibiotic resistance profile of isolated bacteria. Methods. A total of eighty samples of lettuce and ten irrigation waters were collected from the market gardening sites of Boulmiougou, Bissigin, the National School of Public Health and Tanghin. The research of thermotolerant coliforms, Escherichia coli and Salmonella was done following ISO standard methods. Isolated Escherichia coli and Salmonella strains were tested for their resistance profile by using the Kirby-Bauer method with a panel of twenty different antibiotics. Results. Out of the eighty lettuce samples, 68.75% (55) contained thermotolerant coliforms with an average load of 8.75 ×105 CFU g-1. The prevalence of Escherichia coli was 57.5% (46) and Salmonella prevalence was 11.25% (9) including eight strains of Salmonella enterica spp. and one strain of Salmonella enterica arizonea. All irrigation waters were contaminated by thermotolerant coliforms at an average concentration of 3.11×104 CFU/100mL. The prevalence of Escherichia coli in the irrigation waters was 40% (4/10) while Salmonella enterica spp. was 20% (2/10). The highest antibiotic resistance was found in Escherichia coli strains with ampicillin 28.26%, amoxicillin + clavulanic acid 26.09% and tetracycline 19.57%. Conclusion. The water used to irrigate vegetable crops in Ouagadougou could be a major source of contamination of vegetables by microorganisms. In addition, the consumption of lettuce would constitute a risk of propagation of salmonellosis among the population.
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