The aim of this study was to explore the biophysical effects of static magnetic field on osteoblastic cells. MG63 cells were exposed to 0.25 and 0.4-T static magnetic fields (SMF). The cell cycle effects were tested by flow cytometry. The differentiation of the cells was assessed by detecting the changes in prostaglandin E2, osteocalcin, and extracellular matrix expression. Membrane fluidity was used to evaluate the alterations in the biophysical properties of cellular membranes after the SMF simulations. Our results show that SMF exposure increases prostaglandin E2 level and extracellular matrix express in MG63 cells. On the other hand, MG63 cells exposed to 0.4-T SMF exhibited a significant decrease in membrane fluidity at 8 h. Based on these findings, it appears reasonable to suggest that SMF affect osteoblastic maturation by increasing membrane rigidity and then inducing differentiation pathway.
Exposure to an SMF increases the plasma levels of IL-1ra. This effect may inhibit the reduction in PLT in plasma, resulting in prevention in LPS induced DIC.
Recently, the term tensotaxis was proposed to describe the phenomenon that tensile stress or strain affects cell migration. Even so, less attention has been paid to the effects of compressive stress on cell behavior. In this study, by using an injection-molded method combined with photoelastic technology, we developed residual stress gradient-controlled poly-L-lactide discs. After culturing NIH-3T3 fibroblasts on the stress gradient substrate, the cell distributions for high- and low-stress regions were measured and compared. Our results showed that there were significantly more cells in the low-compressive stress region relative to their high-stress analogs (p < 0.05). In addition, NIH-3T3 fibroblasts in the low-compressive stress region expressed more abundant extensive filopodia. These findings provide greater insight into the interaction between cells and substrates, and could serve as a useful reference for connective tissue development and repair.
The aim of this study was to evaluate the effectiveness of vibrational assessment of the mandible fracture patterns. Measurement of natural frequencies and associated vibrational mode shapes was performed to determine the relationship between the dynamic behavior of the human mandible and incidence of mandibular fractures using both in vitro modal testing and finite element analysis. Our results show that the natural frequencies of the human mandible in dry and wet conditions are 567 Hz and 501 Hz, respectively. The first vibrational mode of human mandible is a bending vibration with nodes located at the mandibular body where bone fracture is less likely to occur. By contrast, high vibration amplitudes were identified in the symphysis/parasymphysis and subcondyle regions where bone fractures tend occur. These findings indicate that the vibrational characteristics of the mandible are potential parameters for assessment of the mechanisms of injury.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.