Enhancement of natural killer cell cytotoxicity by using static magnetic field to increase their viability

Lin, Shu Li; Su, Yi; Feng, Sheng; Chang, Wei Jen; Fan, Kan Hsin; Huang, Haw Ming

doi:10.1080/15368378.2019.1591439

Cited by 12 publications

(13 citation statements)

References 46 publications

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“…The selected magnetic field condition deformed HAPF nanocomplexes that can transfer mechanical force to NK cells (Figure S6). , To test the magneto-activated cytolytic function of NK cells, magnetic-field-treated HAPF-NK cells were cocultured with HepG2 cancer cells. Indeed, the HAPF-NK cells treated with the a.c. magnetic field (15 Oe and 1 Hz) for 1 min showed significantly enhanced cytotoxicity against HepG2 cancer cells compared with nonlabeled NK cells without magnetic field application or only F-labeled NK cells at the same magnetic field application (Figure a and Figure S7).…”

Section: Resultsmentioning

confidence: 99%

Magneto-Activation and Magnetic Resonance Imaging of Natural Killer Cells Labeled with Magnetic Nanocomplexes for the Treatment of Solid Tumors

et al. 2021

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Natural killer (NK) cell-based immunotherapy has been considered a promising cell-based cancer treatment strategy with low side effects for early tumors and metastasis. However, the therapeutic efficacy is generally low in established solid tumors. Ex vivo activation of NK cells with exogenous cytokines is often essential but ineffective to generate high doses of functional NK cells for cancer treatment. Image-guided local delivery of NK cells is also suggested for the therapy. However, there is a lack of noninvasive tools for monitoring NK cells. Herein, magnetic nanocomplexes are fabricated with clinically available materials (hyaluronic acid, protamine, and ferumoxytol; HAPF) for labeling NK cells. The prepared HAPF–nanocomplexes effectively attach to the NK cells (HAPF-NK). An exogenous magnetic field application effectively achieves magneto-activation of NK cells, promoting the generation and secretion of lytic granules of NK cells. The magneto-activated HAPF-NK cells also allow an MR image-guided NK cell therapy to treat hepatocellular carcinoma (HCC) solid tumors via transcatheter intra-arterial infusion. Suppressed tumor growth after the treatment of IA infused magneto-activated NK cells demonstrated a potential enhanced therapeutic efficacy of image guided local delivery of magneto-activated HAPF-NK cells. Given the potential challenges of NK cell cancer immunotherapy against established solid tumors, the effective NK cell labeling with HAPF, magneto-activation, and MRI contrast effect of NK cells will be beneficial to enhance the NK cell-therapeutic efficacy in various cancers.

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Section: Resultsmentioning

confidence: 99%

Magneto-Activation and Magnetic Resonance Imaging of Natural Killer Cells Labeled with Magnetic Nanocomplexes for the Treatment of Solid Tumors

et al. 2021

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“…Indeed, SMS induces alterations in the viability of SH-SY5Y cells in response to cisplatin (24), having a modulatory effect on the cell's response to several pharmacological treatments, (42)(43)(44)(45)(46)(47). Other effects of exposure to SMS have already been described in other cellular functions, such as ROS production (24), modulation of redoxrelated enzymes (25), pro-and anti-inflammatory cytokine release (24) and improvement in the killing function of NK cell (17). Given the diversity of the processes affected by SMS, changes in cell viability probably involve processes in addition to cell death, cell cycle distribution and neurotrophin production.…”

Section: Discussionmentioning

confidence: 98%

“…Potential uses and effects of magnetic stimulation over cellular processes have been described, particularly using static magnetic stimulation (SMS). Apart from physiological and homeostatic events, such as wound healing (14), SMS has shown effects in cancer models of glioblastoma (15), adenocarcinoma (16) and leukemia (17), where it has been shown to control the cell cycle, reduce drug resistance to cisplatin and enhance natural killer cell cytotoxicity against tumor cells, respectively. SMS, unlike repetitive transcranial magnetic stimulation (rTMS) -in which changes in the magnetic field create an electric current through electromagnetic induction -does not induce electric currents; however, it has been shown to influence a variety of biological systems (18).…”

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confidence: 99%

Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line

et al. 2020

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Background/Aim: Magnetic stimulation is used in the treatment of a diversity of diseases, but a complete understanding of the underlying mechanisms of action requires further investigation. We examined the effect of static magnetic stimulation (SMS) in different cell lines. Materials and Methods: A culture plate holder with attached NeFeB magnets was developed. Different magnetic field intensities and periods were tested in tumoral and nontumoral cell lines. To verify the cellular responses to SMS, cell viability, cell death, cell cycle and BDNF expression were evaluated. Results: Exposure of SH-SY5Y cells to SMS for 24 hours led to a decrease in cell viability. Analysis 24 h after stimulation revealed a decrease in apoptotic and double-positive cells, associated with an increase in the number of necrotic cells. Conclusion: The effects of SMS on cell viability are cell type-specific, inducing a decrease in cell viability in SH-SY5Y cells. This suggests that SMS may be a potential tool in the treatment of neuronal tumors.Over the years, several electrophysiological studies have expanded the understanding of normal brain activity and its pathological conditions. Technological advances have been an important part of the improvement of therapies and research in several areas such as neurology, psychology, and psychiatry (1). Brain stimulation is a tool for modulating brain function, allowing the association of activity patterns and cognitive function to establish cause-consequence relations (2). Brain stimulation techniques are usually divided into two different types: invasive and non-invasive (NIBS) techniques. Whereas invasive techniques involve greater risk for patients, as demonstrated by studies that compare the impact of different procedures (3, 4), noninvasive techniques have shown favorable results together with lower risks (3, 5). Indeed, NIBS's application has been described in different scenarios: 1. Cognitive improvement on depression (6, 7); 2. Improvement in post-stroke recovery (8); 3. Improvement of the memory and the quality of life of patients with Alzheimer's disease (9, 10); 4. Relief of 5151

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“…10 T SMF exposure can reduce pro‐inflammatory cytokines secreting, such as IL‐6, IL‐8, and TNF‐α, and increase the secretion of anti‐inflammatory factors IL‐10 to some extent 20 . In addition, a previous study reported that 0.4 T SMF increased NK cell cytotoxicity by activating multiple mitogen‐activated protein kinase pathways 21 . As for adaptive immune cells, magnetic exposure remarkably increased the apoptosis of phytohemagglutinin‐stimulated lymphocytes 20 .…”

Section: Introductionmentioning

confidence: 93%

Ultra‐high static magnetic fields cause immunosuppression through disrupting B‐cell peripheral differentiation and negatively regulating BCR signaling

Gu,

Fu,

et al. 2023

MedComm

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To increase the imaging resolution and detection capability, the field strength of static magnetic fields (SMFs) in magnetic resonance imaging (MRI) has significantly increased in the past few decades. However, research on the side effects of high magnetic field is still very inadequate and the effects of SMF above 1 T (Tesla) on B cells have never been reported. Here, we show that 33.0 T ultra‐high SMF exposure causes immunosuppression and disrupts B cell differentiation and signaling. 33.0 T SMF treatment resulted in disturbance of B cell peripheral differentiation and antibody secretion and reduced the expression of IgM on B cell membrane, and these might be intensity dependent. In addition, mice exposed to 33.0 T SMF showed inhibition on early activation of B cells, including B cell spreading, B cell receptor clustering and signalosome recruitment, and depression of both positive and negative molecules in the proximal BCR signaling, as well as impaired actin reorganization. Sequencing and gene enrichment analysis showed that SMF stimulation also affects splenic B cells' transcriptome and metabolic pathways. Therefore, in the clinical application of MRI, we should consider the influence of SMF on the immune system and choose the optimal intensity for treatment.

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Enhancement of natural killer cell cytotoxicity by using static magnetic field to increase their viability

Cited by 12 publications

References 46 publications

Magneto-Activation and Magnetic Resonance Imaging of Natural Killer Cells Labeled with Magnetic Nanocomplexes for the Treatment of Solid Tumors

Magneto-Activation and Magnetic Resonance Imaging of Natural Killer Cells Labeled with Magnetic Nanocomplexes for the Treatment of Solid Tumors

Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line

Ultra‐high static magnetic fields cause immunosuppression through disrupting B‐cell peripheral differentiation and negatively regulating BCR signaling

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