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Abstract.A severe winter storm from January to February 2008 caused considerable damage to the Nanling evergreen broad-leaved forest. The sprout vigor of each species during postdisaster recovery plays a very important role in the succession trends of disturbed forests. In April 2008, four plots (50 × 30 m) were established in the Shierdushui Nature Reserve of Guangdong Province, China, to examine the damage status by the dominant species and diameters at breast height (DBHs), and the sprout response (indicated by the number of sprouts per stem) of the evergreen broad-leaved forest to the severe winter storm. In contrast with freezing damage, the percentage of trees killed by mechanical damage from the mass of snow and ice was significantly higher (10% vs. 90%). More than 70% of the total dead trees had DBHs less than 4 cm. The impact of the severe winter storm in the tree layer was much more serious than in the shrub layer. Stem bending killed the highest percentage of trees, accounting for 59.53% of the total number of dead trees. The sprout number per stem differed significantly among damage types. The more severe damage induced a higher sprout number. Without considering the damage extent, significant differences in the sprout response were also found at both the dominant species and family levels. The tree layer exceeded the shrub layer in the mean number of sprouts (35.87 vs. 15.92). A bivariate correlation indicated that the sprout response was positively related to DBH and annual stem radial growth of the mother trees, implying that DBH and the growth rate of plant species most likely control the sprout response to disturbance in the subtropical evergreen broad-leaved forests in the Nanling Mountains, southern China.

No abstract

Genomic resources including transcriptomic sequences and molecular markers remain scarce in the medicinally important woody legume genus Archidendron F. Mueller. Here we conducted transcriptome sequencing, genic microsatellite marker development, and population diversity analysis in Archidendron clypearia (Jack) I.C. Nielsen. Flower and ower bud transcriptomes were de novo assembled into 173,172 transcripts, with an average transcript length of 1597.3 bp and an N50 length of 2427 bp. A total of 34,701 microsatellite loci were identi ed from 26,716 (15.4%) transcripts. Primer pairs were designed for 718 microsatellite loci, of which 456 (63.5%) were polymorphic. Of the 456 polymorphic markers, 391 (85.7%) and 402 (88.1%) were transferable to A. lucidum (Benth.) I.C. Nielsen and A. multifoliolatum (H.Q. Wen) T.L. Wu, respectively. Using a subset of 15 microsatellite markers, relatively high genetic diversity was detected over two A. clypearia populations, with overall mean expected heterozygosity (H e ) being 0.707 and demonstrating the necessity of conservation. Relatively low differentiation between the two populations was revealed despite the distant separation (about 700 km), with overall inbreeding coe cient of sub-population to the total population (F st ) being 8.7%. This suggests that A. clypearia has mainly an outcrossing mating system and weak genetic structure. These results will offer valuable resources and information for further genetic studies and practical applications in Archidendron and the related taxa.

Genomic resources including transcriptomic sequences and molecular markers remain scarce in the medicinally important woody legume genus Archidendron F. Mueller. Here we conducted transcriptome sequencing, genic microsatellite marker development, and population diversity analysis in Archidendron clypearia (Jack) I.C. Nielsen. Flower and flower bud transcriptomes were de novo assembled into 173,172 transcripts, with an average transcript length of 1597.3 bp and an N50 length of 2427 bp. A total of 34,701 microsatellite loci were identified from 26,716 (15.4%) transcripts. Primer pairs were designed for 718 microsatellite loci, of which 456 (63.5%) were polymorphic. Of the 456 polymorphic markers, 391 (85.7%) and 402 (88.1%) were transferable to A. lucidum (Benth.) I.C. Nielsen and A. multifoliolatum (H.Q. Wen) T.L. Wu, respectively. Using a subset of 15 microsatellite markers, relatively high genetic diversity was detected over two A. clypearia populations, with overall mean expected heterozygosity (He) being 0.707 and demonstrating the necessity of conservation. Relatively low differentiation between the two populations was revealed despite the distant separation (about 700 km), with overall inbreeding coefficient of sub-population to the total population (Fst) being 8.7%. This suggests that A. clypearia has mainly an outcrossing mating system and weak genetic structure. These results will offer valuable resources and information for further genetic studies and practical applications in Archidendron and the related taxa.