The dried root of Salvia miltiorrhiza is a renowned traditional Chinese medicine that was used for over 1000 years in China. Salvianolic acid B (SalB) is the main natural bioactive product of S. miltiorrhiza. Although many publications described the regulation mechanism of SalB biosynthesis, few reports simultaneously focused on S. miltiorrhiza root development. For this study, an R2R3-MYB transcription factor gene (SmMYB52) was overexpressed and silenced, respectively, in S. miltiorrhiza sterile seedlings. We found that SmMYB52 significantly inhibited root growth and indole-3-acetic acid (IAA) accumulation, whereas it activated phenolic acid biosynthesis and the jasmonate acid (JA) signaling pathway. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses revealed that SmMYB52 suppressed the transcription levels of key enzyme-encoding genes involved in the IAA biosynthetic pathway and activated key enzyme-encoding genes involved in the JA and phenolic acid biosynthesis pathways. In addition, yeast one-hybrid (Y1H) and dual-luciferase assay showed that SmMYB52 directly binds to and activates the promoters of several key enzyme genes for SalB biosynthesis, including SmTAT1, Sm4CL9, SmC4H1, and SmHPPR1, to promote the accumulation of SalB. This is the first report of a regulator that simultaneously affects root growth and the production of phenolic acids in S. miltiorrhiza.

MicroRNAs (miRNAs) are important regulators of gene expression involved in plant development and abiotic stress responses. Recently, miRNAs have also been reported to be engaged in the regulation of secondary plant metabolism. However, there are few functional studies of miRNAs in medicinal plants. For this study, we obtained Sm-miR408 interference lines to investigate the function of Sm-miR408 in a medicinal model plant (Salvia miltiorrhiza). It was found that inhibiting the expression of Sm-miR408 could increase the content of salvianolic acid B and rosmarinic acid in the roots. The SmLAC3 and Sm-miR408 expression patterns were analyzed by qRT-PCR. A 5’ RLM-RACE assay confirmed that Sm-miR408 targets and negatively regulates SmLAC3. Moreover, the overexpression of SmLAC3 in S. miltiorrhiza promoted the accumulation of salvianolic acids in the roots. Furthermore, the lignin content of the roots in overexpressed SmLAC3 lines was decreased. Taken together, these findings indicated that Sm-miR408 modulates the accumulation of phenolic acids in S. miltiorrhiza by targeting SmLAC3 expression levels.

Concentric tube robots (CTRs) are a promising prospect for minimally invasive surgery due to their inherent compliance and ability to navigate in constrained environments. Existing mechanics-based kinematic models typically neglect friction, clearance, and torsion between each pair of contacting tubes, leading to large positioning errors in medical applications. In this paper, an improved kinematic modeling method is developed. The effect of clearance on tip position during concentric tube assembly is compensated by the database method. The new kinematic model is mechanic-based, and the impact of friction moment and torsion on tubes is considered. Integrating the infinitesimal torsion of the concentric tube robots eliminates the errors caused by the interaction force between the tubes. A prototype is built, and several experiments with kinematic models are designed. The results indicate that the error of tube rotations is less than 2 mm. The maximum error of the feeding experiment does not exceed 0.4 mm. The error of the new modeling method is lower than that of the previous kinematic model. This paper has substantial implications for the high-precision and real-time control of concentric tube robots.

A new strategy combined gold-coated magnetic nanocomposites assisted enrichment with mass spectrometry was developed for the characterization of disulfide bond-contained proteins from Chinese cobra (Naja atra) venom. In this work, core-shell nanocomposites were synthesized by the seed-mediated growth method and used for the enrichment of snake venom proteins containing disulfide bonds. A total of 3545 tryptic digested peptides derived from 96 venom proteins in Naja atra venom were identified. The venom proteins comprised 14 toxin families including three-finger toxins, phospholipase A 2 , snake venom metalloproteinase, cobra venom factor, and so forth. Extra 16 venom proteins were detected exclusively in the nanocomposites set, among which 11 venom proteins were from the three-finger toxins family. In the present study, the proposed simple and efficient protocol replaced the tedious and laborious technologies commonly used for pre-separating crude snake venom, suggesting widely implementation in low-abundance or trace disulfide bond-contained proteins or peptides characterization.

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Promoting the construction of professional drug inspectors is an important measure to improve the drug regulatory system in China. At present, 31 provinces have basically completed the establishment of professional drug inspectors. In order to use and manage this team well, all provinces have carried out active exploration and hard practice in the construction of professional drug inspectors, and accumulated valuable experience. This paper summarizes Shandong's exploration and experience in recent years in terms of institution building, personnel allocation, capacity improvement, incentive mechanism, assessment and evaluation, integrity building, system building, etc. It analyzes the difficulties encountered in the construction of professional drug inspectors in terms of hierarchical and classified management, professionalization process, assessment and evaluation system, salary and treatment mechanism, etc. On this basis, it puts forward the establishment of evaluation standard system, positive incentive mechanism A series of measures such as optimizing the management system are expected to provide decision-making reference for the construction of professional drug inspectors at the provincial level in the future.

The compound continuum robot employs both concentric tube components and cable-driven continuum components to achieve its complex motions. Nevertheless, the interaction between these components causes coupling, which inevitably leads to reduced accuracy. Consequently, researchers have been striving to mitigate and compensate for this coupling-induced error in order to enhance the overall performance of the robot. This paper leverages the coupling between the components of the compound continuum robot to accomplish specific surgical procedures. Specifically, the internal concentric tube component is utilized to induce motion in the cable-driven external component, which generates coupled motion under the constraints of the cable. This approach enables the realization of high-precision surgical operations. Specifically, a kinematic model for the proposed robot is established, and an inverse kinematic algorithm is developed. In this inverse kinematic algorithm, the solution of a highly nonlinear system of equations is simplified into the solution of a single nonlinear equation. To demonstrate the effectiveness of the proposed approach, simulations are conducted to evaluate the efficiency of the algorithm. The simulations conducted in this study indicate that the proposed inverse kinematic (IK) algorithm improves computational speed by a significant margin. Specifically, it achieves a speedup of 2.8 × 103 over the Levenberg–Marquardt (LM) method. In addition, experimental results demonstrate that the coupled-motion system achieves high levels of accuracy. Specifically, the repetitive positioning accuracy is measured to be 0.9 mm, and the tracking accuracy is 1.5 mm. This paper is significant for dealing with the coupling of the compound continuum robot.