R2R3-MYB Transcription Factor SmMYB52 Positively Regulates Biosynthesis of Salvianolic Acid B and Inhibits Root Growth in Salvia miltiorrhiza

Yang, Rong; Wang, Shengsong; Zou, Haolan; Li, Lin; Li, Yonghui; Wang, Donghao; Xu, Hongxing; Cao, Xiaoyan

doi:10.3390/ijms22179538

Cited by 20 publications

(7 citation statements)

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“…For example, transferring the TF AaORA into A. annua up-regulated the expression of the structural enzyme genes ADS , CYP71AV1 , and DBR2 , and significantly increased the contents of artemisinin and dihydroartemisinic acid [ 34 ]. S. miltiorrhiza SmMYB52 directly binds to and activates the promoters of genes encoding four enzymes in the salvianolic acid B biosynthesis pathway (SmTAT1, Sm4CL9, SmC4H1, SmHPPR1) to promote salvianolic acid B accumulation [ 35 ]. In citrus, CsMADS5 positively regulates carotenoid synthesis by promoting the transcription of the PSY , PDS , and LCYb1 genes [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

Zeng

Junzhong

et al. 2023

IJMS

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Natural pyrethrins have high application value, and are widely used as a green pesticide in crop pest prevention and control. Pyrethrins are mainly extracted from the flower heads of Tanacetum cinerariifolium; however, the natural content is low. Therefore, it is essential to understand the regulatory mechanisms underlying the synthesis of pyrethrins through identification of key transcription factors. We identified a gene encoding a MYC2-like transcription factor named TcbHLH14 from T. cinerariifolium transcriptome, which is induced by methyl jasmonate. In the present study, we evaluated the regulatory effects and mechanisms of TcbHLH14 using expression analysis, a yeast one-hybrid assay, electrophoretic mobility shift assay, and overexpression/virus-induced gene silencing experiments. We found that TcbHLH14 can directly bind to the cis-elements of the pyrethrins synthesis genes TcAOC and TcGLIP to activate their expression. The transient overexpression of TcbHLH14 enhanced expression of the TcAOC and TcGLIP genes. Conversely, transient silencing of TcbHLH14 downregulated the expression of TcAOC and TcGLIP and reduced the content of pyrethrins. In summary, these results indicate that the potential application of TcbHLH14 in improving the germplasm resources and provide a new insight into the regulatory network of pyrethrins biosynthesis of T. cinerariifolium to further inform the development of engineering strategies for increasing pyrethrins contents.

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Section: Discussionmentioning

confidence: 99%

TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

Zeng

Junzhong

et al. 2023

IJMS

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“…The full-length ORF of SmSPL2 were inserted into the Sma I and Kpn I sites of pGreenII-62SK to obtain the effector vector pGreenII- SmSPL2 . The pSm4Cl9 - LUC , pSmTAT1 - LUC , and pSmPAL 1- LUC reporter vectors were obtained in our previous study [ 50 ]. The pair of effector and reporter vector were co-transferred into tobacco leaves, then monitor the transient expression of firefly luciferase (LUC) and renillia luciferase (REN), according to the previous protocol [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Transcription Factor SmSPL2 Inhibits the Accumulation of Salvianolic Acid B and Influences Root Architecture

Wang

Cao

Yang

et al. 2022

IJMS

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The SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) transcription factor play vital roles in plant growth and development. Although 15 SPL family genes have been recognized in the model medical plant Salvia miltiorrhiza Bunge, most of them have not been functionally characterized to date. Here, we performed a careful characterization of SmSPL2, which was expressed in almost all tissues of S. miltiorrhiza and had the highest transcriptional level in the calyx. Meanwhile, SmSPL2 has strong transcriptional activation activity and resides in the nucleus. We obtained overexpression lines of SmSPL2 and rSmSPL2 (miR156-resistant SmSPL2). Morphological changes in roots, including longer length, fewer adventitious roots, decreased lateral root density, and increased fresh weight, were observed in all of these transgenic lines. Two rSmSPL2-overexpressed lines were subjected to transcriptome analysis. Overexpression of rSmSPL2 changed root architectures by inhibiting biosynthesis and signal transduction of auxin, while triggering that of cytokinin. The salvianolic acid B (SalB) concentration was significantly decreased in rSmSPL2-overexpressed lines. Further analysis revealed that SmSPL2 binds directly to the promoters of Sm4CL9, SmTAT1, and SmPAL1 and inhibits their expression. In conclusion, SmSPL2 is a potential gene that efficiently manipulate both root architecture and SalB concentration in S. miltiorrhiza.

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“…SmbHLH148 and SmMYB1 regulate phenolic acid biosynthesis by activating the expression of downstream genes, such as PAL1, C4H1, TAT, HPPR, RAS, and CYP98A14 (Xing et al, 2018;Zhou et al, 2021b). SmMYB52 simultaneously affects the production of phenolic acids by binding to the MBE elements in promoter regions of SmTAT1, Sm4CL9, SmC4H1, and SmHPPR1 (Yang et al, 2021b). Furthermore, SmbHLH3 acts a repressor in the biosynthesis of phenolic acids in S. miltiorrhiza hairy roots by reducing the expression of DXS3, DXR, HMGR1, KSL1, CPS1 and CYP76AH1 (Zhang et al, 2020).…”

Section: Transcription Factors Involved In the Biosynthesis Of Phenol...mentioning

confidence: 99%

Environmental and Genetic Factors Involved in Plant Protection-Associated Secondary Metabolite Biosynthesis Pathways

Zhan

Chen

et al. 2022

Front. Plant Sci.

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Plant specialized metabolites (PSMs) play essential roles in the adaptation to harsh environments and function in plant defense responses. PSMs act as key components of defense-related signaling pathways and trigger the extensive expression of defense-related genes. In addition, PSMs serve as antioxidants, participating in the scavenging of rapidly rising reactive oxygen species, and as chelators, participating in the chelation of toxins under stress conditions. PSMs include nitrogen-containing chemical compounds, terpenoids/isoprenoids, and phenolics. Each category of secondary metabolites has a specific biosynthetic pathway, including precursors, intermediates, and end products. The basic biosynthetic pathways of representative PSMs are summarized, providing potential target enzymes of stress-mediated regulation and responses. Multiple metabolic pathways share the same origin, and the common enzymes are frequently to be the targets of metabolic regulation. Most biosynthetic pathways are controlled by different environmental and genetic factors. Here, we summarized the effects of environmental factors, including abiotic and biotic stresses, on PSM biosynthesis in various plants. We also discuss the positive and negative transcription factors involved in various PSM biosynthetic pathways. The potential target genes of the stress-related transcription factors were also summarized. We further found that the downstream targets of these Transcription factors (TFs) are frequently enriched in the synthesis pathway of precursors, suggesting an effective role of precursors in enhancing of terminal products. The present review provides valuable insights regarding screening targets and regulators involved in PSM-mediated plant protection in non-model plants.

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R2R3-MYB Transcription Factor SmMYB52 Positively Regulates Biosynthesis of Salvianolic Acid B and Inhibits Root Growth in Salvia miltiorrhiza

Cited by 20 publications

References 50 publications

TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

Transcription Factor SmSPL2 Inhibits the Accumulation of Salvianolic Acid B and Influences Root Architecture

Environmental and Genetic Factors Involved in Plant Protection-Associated Secondary Metabolite Biosynthesis Pathways

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