Biological wastewater treatment is not effective in removal of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this study, we fabricated a photocatalytic reactive membrane by functionalizing polyvinylidene fluoride (PVDF) ultrafiltration (UF) membrane with titanium oxide (TiO) nanoparticles for the removal of ARB and ARGs from a secondary wastewater effluent. The TiO-modified PVDF membrane provided complete retention of ARB and effective photocatalytic degradation of ARGs and integrons. Specifically, the total removal efficiency of ARGs (i.e., plasmid-mediated floR, sul1, and sul2) with TiO-modified PVDF membrane reached ∼98% after exposure to UV irradiation. Photocatalytic degradation of ARGs located in the genome was found to be more efficient than those located in plasmid. Excellent removal of integrons (i.e., intI1, intI2, and intI3) after UV treatment indicated that the horizontal transfer potential of ARGs was effectively controlled by the TiO photocatalytic reaction. We also evaluated the antifouling properties of the TiO-UF membrane to demonstrate its potential application in wastewater treatment.
To combat the spread of antibiotic
resistance into the environment,
we should adequately manage wastewater effluent treatment to achieve
simultaneous removal of antibiotics, antibiotic resistant bacteria
(ARB), and antibiotic resistance genes (ARGs). Herein, we fabricate
a multifunctional electroactive poly(vinylidene fluoride) ultrafiltration
membrane (C/PVDF) by phase inversion on conductive carbon cloth. The
membrane possesses not only excellent retention toward ARB and ARGs
but also exhibits high oxidation capacity as an electrode. Notably,
sulfamethoxazole degradation involving hydroxylation and hydrolysis
by the anode membrane is predominant, and the degradation efficiency
is up to 81.5% at +4 V. Both electro-filtration processes exhibit
significant ARB inactivation, anode filtration is superior to cathode
filtration. Moreover, the degradation of intracellular ARGs (iARGs)
located in the genome is more efficient than those located in the
plasmid, and these degradation efficiencies at −2 V are higher
than +2 V. The degradation efficiencies of extracellular ARGs (eARGs)
are opposite and are lower than iARGs. Compared with regular filtration,
the normalized flux of electroactive ultrafiltration membrane is improved
by 18.0% at −2 V, 15.9% at +2 V, and 30.4% at +4 V during treating
wastewater effluent, confirming its antifouling properties and feasibility
for practical application.
Biofouling is a multifaceted and
unavoidable problem in the application
of membrane separation technology. Here, we functionalized polyvinylidene
fluoride (PVDF) ultrafiltration membranes with poly(ionic liquid)
(PIL) brushes to provide them with antibiofouling properties. The
PIL brush grafted membranes (PIL-M) were prepared via atom transfer
radical polymerization (ATRP) using different ionic liquids (ILs)
on the membrane surface. Four functionalized membranes with different
alkyl chain lengths (C4-M, C8-M, C12-M, and C16-M) were prepared to explore the relationship
between surface structure and antibacterial properties. Our results
showed that all of the PIL-M had antibacterial capabilities with the
highest efficiency of 84.6% for the C12-M. Moreover, the
antibacterial performance was improved by increasing the ATRP reaction
temperature and time. Liposome vesicles were used as the bacterial
cell membrane model to evaluate the antibacterial membrane damage
mechanism. IL and PIL brushes could damage cell membranes through
disrupting the lipid bilayer with longer alkyl chains associated with
an enhanced effect. Zeta potential measurements showed that the interference
of electrostatic interactions with bacteria also played an important
role in the bactericidal mechanism. Moreover, filtration experiments
in a cross-flow system further indicated that PIL-M membranes have
favorable antibiofouling performance, with a stable flux increase
41.7% larger than that of the pristine PVDF membrane. Our results
suggest that functionalization of the membrane surface with the PIL
brushes can effectively resist bacteria and thereby significantly
mitigate biofouling on the PVDF membranes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.