Objective:Highly variable genome of HCV and high prevalence in many geographical areas made it necessary to conduct local population studies. This study has been conducted to show HCV parameters along with clinical features in the local population of interior Sindh, province of Pakistan.Methods:Present study was conducted in from August 2010 to November 2015 in the rural areas of Sindh, Pakistan. All the 31560 screened samples selected for the study were tested by second Generation Enzyme Linked Immunosorbent Assay (ELISA Biokit 480&96).Results:Total 31560 people were screened for HCV and out of these 13.67% (n= 4314) HCV infected patients. When 4314 samples of patients were examined; the anti-HCV was significantly higher in males 2814 (14.98%) than in females 1500 (11.74%) with P value = 0.06. The age of the patients ranged from 18 to 65 years. Out of 4314 HCV samples, 3020 (70%) were of Genotype 3a, 237(5.5%) of Genotype 2a, 108 (2.5%) of Genotype- 1a, 216 (5%) of Genotype 1b, 237 (5.5%) of Genotype 3b and 43 (1%) of Genotype 4. Additionally, 108 (2.5%) had co-infection and 345 (8%) samples showed no result –designated as untypable by the genotyping.Conclusion:This study showed that HCV is most frequently reported disease with genotype 3a being the most prevalent genotype.
Objective: This study was done to determine the association between lipid levels and diabetic Indices in confirm patients of myocardial infarction with versus without diabetes mellitus type II. Methodology: Two hundred acute myocardial infarction having ST-segment elevation patients with diabetes mellitus type II and without diabetes mellitus type II were enrolled. Lipid levels and diabetic indices were measured, and the correlations among them were analyzed. Results: Positive correlations were found between triglycerides and fasting blood sugar, low density lipoprotein and Insulin when compared among myocardial infarction patients. Comparison among myocardial infarction patients with diabetes mellitus type II and without diabetes mellitus type II, negative correlations were found between triglycerides and glycosylated hemoglobin (HbA1C), low density lipoprotein and glycosylated hemoglobin, glycosylated hemoglobin and Insulin. Conclusion: Correlations between lipid levels and diabetic indices when compared were found to be Positive in myocardial infarction patients only and Negative when compared between myocardial infarction patients with diabetes mellitus type II and without diabetes mellitus type II.
Hepatitis C virus (HCV) chronically infects almost 2% of world's population. Chronic infection can lead to liver failure and hepatocellular carcinoma (HCC). Approximately 10% of the Pakistani population is infected with HCV and type 3 is the most prevalent genotype with 75-90% prevalence. In this study we have developed transiently expressing cell culture based system for the expression of HCV non-structural NS3, NS3-4A and NS4A proteins of genotype 3a. HCV non-structural genes NS3, NS3-4A and NS4A were cloned in to pFLAG-CMV2 and pEGFP-C1vectors. All vectors were transfected separately to Huh-7 cells and their protein expression was analyzed by Western blot and immunofluorescence. All proteins were expressed correctly and in the transfection we have obtained 42-70% efficiency for all clones. This system can be used for the development of novel antiviral strategies to inhibit the viral replication, to study apoptosis pathways induced by HCV, for the evaluation of vaccine candidates and also to study the role of HCV different signaling pathways.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.