Cyclic nucleotide gated ion channels (CNGCs) in plants have very important role in signaling and development. The study reports role of CNGC19 and CNGC20 in salt stress in
A. thaliana. In-silico,
genome wide analysis showed that
CNGC19
and
CNGC20
are related to salt stress with maximum expression after 6 h in
A. thaliana
. The position of inserted T-DNA was determined (
in-vivo)
through TAIL-PCR for activation tagged mutants of
CNGC19
and
CNGC20
under salt stress. The expression of
AtCNGC19
and
AtCNGC20
after cloning under 35S promoter of expression vectors pBCH1 and pEarleyGate100 was determined in
A. thaliana
by real-time PCR analysis. Genome wide analysis showed that AtCNGC11 had lowest and AtCNGC20 highest molecular weight as well as number of amino acid residues.
In-vivo
expression of
AtCNGC19
and
AtCNGC20
was enhanced through T-DNA insertion and 35S promoter in over-expressed plants under high salt concentration.
AtCNGC19
was activated twice in control and about five times under 150 mM NaCl stress level, and expression value was also higher than
AtCNGC20
. Phenotypically, over-expressed plants and calli were healthier while knock-out plants and calli showed retarded growth under salinity stress. The study provides new insight for the role of AtCNGC19 and AtCNGC20 under salt stress regulation in
A. thaliana
and will be helpful for improvement of crop plants for salt stress to combat food shortage and security.
A comparative analysis of sorted semiconducting (sc‐) single‐walled carbon nanotubes (SWCNTs) films, metallic (m‐) SWCNTs films, pristine SWCNTs films, and graphene‐based simple two‐terminal sensors for the applications of ammonia gas sensing is presented. The comparison of the sensing response of different devices by separately measuring the SWCNTs film resistance and the contact resistance between SWCNTs and the electrodes reveals that the performance mainly relies on the modification of tube conductivity under exposure to gas. Moreover, the measurements show that the highest sensitivity of the devices is achieved by use of sc‐SWCNTs as the conducting channels. Sensor coated with surfactant‐free sc‐SWCNTs shows a sensitivity of 0.78% ppm at 5–100 ppm ammonia (NH3) concentrations that happened to be ≈28, ≈22, and ≈173 times more sensitive than pristine‐SWCNTs, m‐SWCNTs, and graphene‐based sensors, respectively, at 50 ppm and ≈4.5 times the previously reported sc‐SWCNTs‐based sensor at 5 ppm. Notably, all the experiments values are achieved at room temperature without any extra heating treatment for the recovery process. These results show that surfactant‐free sc‐SWCNTs provide a promising way of creating sensors with improved selectivity and sensitivity, which predicts the auspicious prospects in the development of future applications.
Gel chromatography is used to separate single-chirality and selective-diameter SWCNTs. We also explore the use of photothermal therapy and biosensor applications based on single-chirality, selected-diameter, and unique geometric shape.
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