Molecular characterization and expression of cyclic nucleotide gated ion channels 19 and 20 in Arabidopsis thaliana for their potential role in salt stress

Abstract

Cyclic nucleotide gated ion channels (CNGCs) in plants have very important role in signaling and development. The study reports role of CNGC19 and CNGC20 in salt stress in A. thaliana. In-silico, genome wide analysis showed that CNGC19 and CNGC20 are related to salt stress with maximum expression after 6 h in A. thaliana . The position of inserted T-DNA was determined ( in-vivo) through TAIL-PCR for activation tagged mutants of CNGC19 and CNGC20 under salt stress. The expression of AtCNGC19 and AtCNGC20 after cloning under 35S promoter of expression vectors pBCH1 and pEarleyGate100 was determined in A. thaliana by real-time PCR analysis. Genome wide analysis showed that AtCNGC11 had lowest and AtCNGC20 highest molecular weight as well as number of amino acid residues. In-vivo expression of AtCNGC19 and AtCNGC20 was enhanced through T-DNA insertion and 35S promoter in over-expressed plants under high salt concentration. AtCNGC19 was activated twice in control and about five times under 150 mM NaCl stress level, and expression value was also higher than AtCNGC20 . Phenotypically, over-expressed plants and calli were healthier while knock-out plants and calli showed retarded growth under salinity stress. The study provides new insight for the role of AtCNGC19 and AtCNGC20 under salt stress regulation in A. thaliana and will be helpful for improvement of crop plants for salt stress to combat food shortage and security.

Genome-Wide Identification and Expression Analysis of BraGLRs Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction

“…Previous studies have reported that plant AtCNGCs engage in biotic and abiotic stresses and that AtCNGC19 expression is elevated at increased NaCl concentrations. AtCNGC19 is involved in the tolerance of A. thaliana to high salt concentrations [ 49 , 50 ]. Similarly, BraGLR1 may help respond to salt stress by interacting with CNGC family members.…”

Calcium channels and transporters: Roles in response to biotic and abiotic stresses

“…Under hot/cold and drought stresses, atcngc16 resulted in a greater than 10-fold stress-dependent reduction in pollen fitness and seed set ( Tunc-Ozdemir et al, 2013 ). Arabidopsis transgenic overexpressing AtCNGC19 or AtCNGC20 displayed salt tolerance and their knockout plants become more sensitive to the salt stress ( Oranab et al, 2021 ). Some AtCNGCs involved in the uptake and transport of heavy metal ions such as Pb 2+ and Cd 2+ .…”

“…When two or more channels create a complex and function as one unit, they are marked together. become more sensitive to the salt stress (Oranab et al, 2021). Some AtCNGCs involved in the uptake and transport of heavy metal ions such as Pb 2+ and Cd 2+ .…”

Genome-Wide Analysis and Expression of Cyclic Nucleotide–Gated Ion Channel (CNGC) Family Genes under Cold Stress in Mango (Mangifera indica)

“…For example, 15 AtCNGCs ( AtCNGC1-10 / 12 / 14 / 16 / 18 / 20 ) are encoded Ca 2+ -permeable channels [ 8 ], and numerous studies confirm their functions in plant development, such as AtCNGC2 in leaf senescence, AtCNGC3 in germination, AtCNGC5 / 6 / 9 in root hair development, AtCNGC7 / 8 in male fertility, AtCNGC14 in root gravitropism and AtCNGC16 / 18 in pollen development [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Several AtCNGCs are involved in stress response, including AtCNGC1 / 10 / 19 / 20 in salt stress, AtCNGC2 / 4 / 11 / 12 in abiotic stress, AtCNGC2 / 5 / 6 / 9 / 12 in stomatal defense and AtCNGC6 in heat stress [ 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. Together, these findings provide valuable references for functional characterization and application of CNGC genes in non-model plant species.…”