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Background: The object of this work was to evaluate of the hepatic effects of the herbicides glyphosate-Roundup® by different doses in both sexes of albino rats. Methods: Forty animals divided into four groups with ten animals for each (both sexes) were treated orally with vehicle (controls) and 25, 50, and 100 mg/kg bwt of glyphosate-Roundup® (treated groups) for 15 days daily. Results: The most conspicuous changes occurred on the liver treated groups due to glyphosate toxicity were the increase of enzymes activities of ALT and AST, cellular infiltration, many signs of nucleus degeneration, focal necrosis, rarified cytoplasm, disorganization of cellular organelles, and deposition of lipid droplets. The increase in the amount of collagenous fibers and the number of the mast cell were also observed. Conclusion: Our results indicated that the administration of glyphosate-Roundup® in different doses may cause adverse effects on the histopathological, ultrastructure, and biochemical alternations on the liver of the albino rats.

Background: This work was designed to study the histological and ultrastructure abnormalities in the liver induced by high dose of aspartame (ASP) in normal and diabetic rats. Methods: Forty adult male rats were used in this study and divided into four groups with ten animals for each. Rats of group one served as controls and administered vehicle. Rats of group two were administered aspartame (ASP) at 200 mg/Kg bwt once daily for 4 weeks. Rats of group three (D) were given streptozotocin (STZ) one time at a dose of 70 mg/Kg bwt. The fourth group (D+ASP) was represented by diabetic rats administered with aspartame at 200 mg/Kg bwt. Results: The most obvious changes that occurred in the liver treated rats were destruction of hepatocytes which led to necrosis, the appearance of large areas of rarified cytoplasm separating dense areas of cellular organelles, disorganization of cellular organelles, increase in collagenous fibers and depletion of polysaccharides (carbohydrate content of hepatocytes). Conclusions: Our results indicated that ASP administration may be responsible for the morphological alternations of liver in both normal and diabetic animals.

This study aimed to determine the effect of gallic acid (GA) on ameliorating bisphenol A (BPA) nephrotoxicity in male rat kidneys. Forty rats were assigned randomly into two groups: control (ten animals) and BPA (40 mg/kg bwt) (thirty animals), the second group was divided into three subgroups: BPA alone, BPA + G50 (50 mg/kg bwt), and BPA + G200 (200 mg/kg bwt). The biochemical analysis included measurements of the contents of nitric oxide, lipid peroxidation, reactive oxygen species, and cytokines (interleukin-1α and interleukin-6) in the kidney. The antioxidant enzymes catalase and superoxide dismutase were also measured in the kidney. Kidney function was assessed by determining uric acid, urea, and creatinine levels. The morphological investigations included hematoxylin and eosin staining for assessing the general histology and determining the glomerular and corpuscular areas, the tubular cell degeneration mean area, and the mean leukocyte infiltration area. Also, collagen fiber intensity and polysaccharide content were analyzed. Furthermore, immunohistochemical, morphometric, and ultrastructural studies were carried out. The results revealed morphological, immunohistochemical, and biochemical alterations in the kidney. Most of these changes showed a satisfactory improvement of kidney damage when BPA-administered rats were treated with GA at both doses. In conclusion, GA exhibited a strong protective effect against BPA-induced nephrotoxicity.

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