The hydroalcoholic extract of Fabiana imbricata inhibited the enzyme /3-glucuronidase (B-gluc) in vitro. Bioassay-guided isolation led to scopoletin as the main active constituent of F . imbricafa. Scopoletin was a noncompetitive inhibitor of p-D-glucuronidase with a Ki value of 4 X M. A single oral dose of 250 mg/kg body weight F. imbricata extract produced a significant increase (pCO.05) in the urine output of rats. The diuretic effect of the extract was weak in comparison with hydrochlorothiazide at 25 mglkg. In the acute oral toxicity study in rats, 'Pichi' was shown to be a low toxicity crude drug at doses up to 5 g crude extract/kg body weight. At concentrations up to 0.50 mg/mL, the crude extract did not increase the number of chromosome aberrations in the in uitro human lymphocyte assay.
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