β‐glucuronidase inhibition and diuretic activity of <i>Fabiana imbricata</i> R. &amp; P. (Solanaceae)

Schmeda-Hirschmann, Guillermo; Loyola, J. I.; Reyes, Sergio; Hubert, E; Rodríguez, Marcela A.; Rodrı́guez, Jaime A.; Dutra-Behrens, M.

doi:10.1002/ptr.2650080810

Cited by 10 publications

(2 citation statements)

References 11 publications

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“…b-Glucuronidase. b-Glucuronidase (b-glucur) activity was measured spectrophotometrically using p-nitrophenyl-b D-glucuronide as substrate as reported by Schmeda-Hirschmann et al (1994). The inhibitor glucosaccharo-1:4 lactone was used as a control.…”

Section: Enzyme Inhibitionmentioning

confidence: 99%

Antimicrobial Butyrolactone I Derivatives from the Ecuadorian Soil Fungus Aspergillus terreus Thorn. var terreus

Cazar

Schmeda-Hirschmann

Astudillo

2005

World J Microbiol Biotechnol

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The fungus Aspergillus terreus Thorn var. terreus isolated from an Ecuador soil sample was cultured in liquid and solid media and yielded three main metabolites identified as terreic acid (1), butyrolactone I (2) and lovastatin (3). The natural products as well as three synthetic butyrolactone I derivatives were assessed for antimicrobial activity against Gram-positive and Gram-negative bacteria and fungi as well as for seed germination and seedling growth. Furthermore, the compounds were assessed as inhibitors towards the enzymes acetylcholinesterase, b-glucosidase, and b-glucuronidase. Terreic acid, butyrolactone I, butyrolactone 4¢,4¢¢-diacetate (2.1), and 3¢-(3-methylbutyl)-butyrolactone II (2.2) were active towards the phytopathogenic bacteria Erwinia carotovora with IC 50 of 5 and 4-18 lg/ml, respectively. Under the same experimental conditions, the IC 50 of streptomycin was 1.9 lg/ml. 3¢-(3-Methylbutyl)-butyrolactone II was moderately active against Pseudomonas syringae and Botrytis cinerea with IC 50 of 21lg/ml and MIC of 15.6 lg/ml, respectively. Butyrolactone I also inhibited germination of the dicot Lactuca sativa with an IC 50 of 5 · 10 )5 M. The IC 50 of reference herbicide acetochlor was 1 · 10 )5 M. The effect of 2.2 and 2.3, known as butyrolactone III on Panicum millaceum germination and growth was stronger than that of 2 and 2.1. Reduction of the double bond in the isoprenyl side chain of butyrolactone I increased the antibacterial effect against E. carotovora as well as acetylation. To our best knowledge, this is the first report on the antibacterial effect of butyrolactone derivatives towards Erwinia carotovora and the phytopathogenic fungus Botrytis cinerea. The butyrolactone I derivative 2.2 presented a moderate inhibitory effect against the enzyme acetylcholinesterase with an IC 50 of 47 lg/ml. Under the same experimental conditions, the reference inhibitor galanthamine had an IC 50 of 3 lg/ml.

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Section: Enzyme Inhibitionmentioning

confidence: 99%

Antimicrobial Butyrolactone I Derivatives from the Ecuadorian Soil Fungus Aspergillus terreus Thorn. var terreus

Cazar

Schmeda-Hirschmann

Astudillo

2005

World J Microbiol Biotechnol

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“…Scopoletin has shown strong inhibition of acetylcholinesterase and thus it is a potential candidate for Alzheimer's disease treatment (18). Furthermore, scopoletin has been introduced as inhibitor of β-glucuronidase, peroxidase, acetylcholinesterase, and mono amine oxidase (19)(20)(21)(22).…”

Section: Modification Of Ldh Enzyme Can Induce Conformationalmentioning

confidence: 99%

Scopoletin and Morin Inhibit Lactate Dehydrogenase Enzyme Activity, Which Is Critical for Cancer Metabolism

Mazlaghaninia

Atri

Seyedalipour

2019

Hormozgan medical journal

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Background: Lactate dehydrogenase (LDH) is a tetrameric enzyme that catalyzes the interconversion of pyruvate to L-lactate. The importance of this enzyme is because LDH isoenzymes are involved in cancer, heart, and liver diseases. Inhibition of this enzyme can help prevent and treat different diseases. Morin is a flavonoid found in the Moraceae family and scopoletin is a coumarin found in Scopolia genus. Objectives: The aim of this study was to determine the effect of morin and scopoletin as two natural products on the activity and structure of lactate dehydrogenase enzyme. Methods: Morin and scopoletin were examined for inhibition of the activity of LDH in 100 mM sodium phosphate buffer pH 7.5, at room temperature using UV-V spectrophotometry. Fluorescence spectroscopy was used to characterize protein structural changes in the presence of morin and scopoletin. Results: Km and Vmax of LDH for pyruvate were 11.69 mM and 1.258 mM/min, respectively. The kinetic results showed that morin and scopoletin are LDH inhibitors. The Ki values of morin and scopoletin were determined as 1.78 µM and 0.8 µM, respectively, using a secondary plot. Fluorescence intensity quenching and red shift of the maximum wavelength of emission in a concentrationdependent manner showed that morin and scopoletin bind to LDH and affect its structure. Conclusions: The results suggest that morin and scopoletin bind to LDH, influence its conformation and inhibit its activity. Scopoletin showed more effective inhibition of LDH activity and it can be a promising candidate in the field of tumor metabolism inhibitors.

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Fabiana imbricata Ruiz & Pav.

González

2021

Medicinal and Aromatic Plants of the World

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β‐glucuronidase inhibition and diuretic activity of Fabiana imbricata R. & P. (Solanaceae)

Cited by 10 publications

References 11 publications

Antimicrobial Butyrolactone I Derivatives from the Ecuadorian Soil Fungus Aspergillus terreus Thorn. var terreus

Antimicrobial Butyrolactone I Derivatives from the Ecuadorian Soil Fungus Aspergillus terreus Thorn. var terreus

Scopoletin and Morin Inhibit Lactate Dehydrogenase Enzyme Activity, Which Is Critical for Cancer Metabolism

Fabiana imbricata Ruiz & Pav.

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