The toxicological characteristics of the injection form of copper nanopowder are presented. The studied compound was administered in carts of 500 mg /kg (group 2 animals), 1000 mg/kg (group 3 animals), 2000 mg/kg (group 4 animals), 3000 mg/kg (group 5 animals) and 5000 mg/kg (group 6 animals). Animals of the control group (group 1) were injected with an appropriate amount of solvent (propylene glycol). The volume of the injected compound did not exceed 5 ml. It was found that after administration of the studied compound at doses of 500 and 1000 mg/kg, there were no clinical manifestations of intoxication in white rats. With an increase in doses up to 2000 – 5000 mg / kg of body weight, anxiety, difficulty breathing, and tremor were observed. However, according to the results of the experiment, we did not establish a lethal dose of the drug. When studying the chronic toxicity of the drug, negative changes in animal behavior were observed for 14 days. During the whole time of the experiment, for some time there were not coordinated and coordinated movements. There was no paralysis. The mucous membranes were pink in color without pathological changes. Throughout the entire time of the research, the animals reacted to external (sound) stimuli. A decrease or lack of appetite was observed an hour after administration of the drug and lasted for 6 days, then returned to normal. Thus, the studied compound can be attributed to hazard class IV and to the group of low-toxic substances in accordance with GOST 12.1.007-76.
The article is devoted to the study of the effect of a compound based on an aqueous solution of fullerene C60 on the hematological parameters of white rats. The experiment was carried out on three groups of animals. The first group served as a control. The second group was injected with 1 ml of an aqueous solution of Fullerene C60 (1 mg / ml of DV), 150 mg of resveratrol – 25 mg, betoin hydrochloride – 10 mg. The third group received 1 ml of an aqueous solution of Fullerene C60 (1 mg/ml of DV), 2500 mg of resveratrol, 1000 mg of betoin hydrochloride. It was determined that the animals of the second group had a significant increase in the number of erythrocytes by 11.8% and the hemoglobin content by 14.8% relative to the control values. In white rats of the third group, there were no significant differences with the control. In animals of the second group, the number of rod-shaped neutrophils and monocytes increased by 10 and 9.3%, respectively, relative to the control. No significant differences were found in animals of the third group. The number of segmented neutrophils after the introduction of an aqueous solution of fullerene C60 increased by 12.4% (group 2 animals) and 6.1% (group 3 animals). It was revealed that the compound based on an aqueous solution of fullerene C60 has a pronounced effect on the hematological parameters of the body of white rats. The most effective dose was 1 ml of an aqueous solution of Fullerene C60 (1 mg / ml of DV), 150 mg of resveratrol – 25 mg, betoin hydrochloride – 10 mg, after administration of which an increase in the number of erythrocytes by 11.8% and the hemoglobin content by 14.8% relative to the control values, the number of rod-shaped neutrophils and monocytes by 10 and 9.3%, respectively, and the number of segmented neutrophils by 12.4%.
New data on the mechanisms of pain syndrome development in dogs and cats were obtained, in particular, the role of anti - inflammatory cytokines: interleukin-4 (IL-4), interleukin-6 (IL - 6), interferon - gamma (IFN - gamma), tumor necrosis factor - alpha (TNF-alfa) in the formation and manifestation of this pathology was determined. The results obtained allow us to assess the dynamics of the immune status of animals with pain syndrome. Cytokines: IL-4, IL-6, and IFN - gamma showed high significance in the diagnosis of pain syndrome. Thus, the values of IL-4 in the cerebrospinal fluid and blood serum in dogs in the first experimental group were 3.5 times and 3.4 times higher in the second experimental group than in the control, in cats of the first experimental group-4.3 and 4.6 times in the second experimental group. As well as IL-4, both IL - 6 and IFN - gamma showed their high activity, in contrast to TNF-alfa, which was less sensitive to changes in pain syndrome.
The aim of this work was to study the dynamics of clinical indicators biohimicheskogo serum carnivores in the relief of pain drugs, "Gabapentin" and "Flexiprobe». For the study, a control group and two experimental groups were formed on the principle of pairs of analogues of 34 animals (17 dogs and 17 cats) in each. Intact animals (n=34, dogs-17, cats-17) were intramuscularly adminis-tered the drug "Sodium chloride 0.9 %" at a dose of 1 ml/kg at intervals of 24 hours for 7 days. An-imals of the experimental groups with pain syndrome were treated according to the following scheme: in the first experimental group (n=34, dogs-17, cats-17), the drug "Gabapentin" was used orally or rectally as a drug for the correction of pain syndrome in a dose of 10-60 mg/kg at intervals of 24 hours for 7 days, the optimal dose was selected depending on the type and severity of pain. In the second experimental group (n=34, dogs-17 heads, cats-17 heads), the drug "Flexoprofen" was administered subcutaneously or intramuscularly at a dose of 2 mg/kg at intervals of 24 hours for 7 days to correct the pain syndrome. The diagnostic marker of pain syndrome is a violation of electro-lyte metabolism in the blood serum in dogs and cats, characterized by a significant increase in sodi-um levels from 138.2±3.7 mmol/l to 194 ±4.2 mmol/l and a decrease in calcium levels to 2.4±0.7 mmol/l in dogs and 2.3±0.8 mmol/l in cats. The maximum therapeutic effectiveness of drugs with the least number of side effects is achieved by multimodal use of the drugs "Gabapentin"and " Flexoprofen".
The article presents the results of studies on the effect of commercial food on the processes of free radical oxidation of lipids in the body of cats with urinary tract pathology. It has been found that the concentration of diene conjugates in the blood serum of sick animals increases significantly by 15.1 - 36.7% relative to healthy ones. The MDA content in the blood serum of healthy animals was 6.05 ± 0.28 nmol / g. In animals with urolithiasis, this indicator increased by about 2 times. When feeding with commercial food and homemade food, LPO processes normalize, which is most pronounced on the 21st day. The average activity of catalase in the blood serum of sick animals was increased from 30.6% to 40.2% relative to healthy ones (15.15 ± 0.61 ?mol / ml). After feeding with therapeutic and prophylactic fodders, by the 7th day, the catalase activity did not significantly decrease compared to the initial level, but remained higher than in control animals by 19.6% - 37.1%. On the 14th day, the catalase activity did not change significantly compared with the 7th day and was higher than the control values by 21.5% - 27.5%.
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