Context:Ferulago (Apiaceae) species have been used since ancient times for the treatment of intestinal worms, hemorrhoids, and as a tonic, digestive, aphrodisiac, or sedative, as well as in salads or as a spice due to their special odors.Objectives: This study reports the α-amylase and α-glucosidase inhibitory activities of dichloromethane extract and bioactive compounds isolated from Ferulago bracteata Boiss. & Hausskn. roots.Materials and methods: The isolated compounds obtained from dichloromethane extract of Ferulago bracteata roots through bioassay-guided fractionation and isolation process were evaluated for their in vitro α-amylase and α-glucosidase inhibitory activities at 5000–400 µg/mL concentrations. Compound structures were elucidated by detailed analyses (NMR and MS).Results: A new coumarin, peucedanol-2′-benzoate (1), along with nine known ones, osthole (2), imperatorin (3), bergapten (4), prantschimgin (5), grandivitinol (6), suberosin (7), xanthotoxin (8), felamidin (9), umbelliferone (10), and a sterol mixture consisted of stigmasterol (11), β-sitosterol (12) was isolated from the roots of F. bracteata. Felamidin and suberosin showed significant α-glucosidase inhibitory activity (IC50 0.42 and 0.89 mg/mL, respectively) when compared to the reference standard acarbose (IC50 4.95 mg/mL). However, none of the tested extracts were found to be active on α-amylase inhibition.Discussion and conclusions: The present study demonstrated that among the compounds isolated from CH2Cl2 fraction of F. bracteata roots, coumarins were determined as the main chemical constituents of this fraction. This is the first report on isolation and characterization of the bioactive compounds from root extracts of F. bracteata and on their α-amylase and α-glucosidase inhibitory activities.

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Obesity is a serious disease that can be due to genetic and environmental reasons and is defined by the World Health Organization as abnormal or excessive fat accumulation that may impair health. There is an increasing trend towards herbal slimming products in obesity treatment. The quality control analysis of these products is important for public health. In this study, new, simple, and sensitive high performance liquid chromatography methods were developed for qualitative and quantitative determination of arbutin, hydroquinone, ursolic acid, chlorogenic acid, epigallocatechin gallate, epigallocatechin, epicatechin gallate, and catechin in eleven herbal slimming products in Turkey containing Camellia sinensis, Ilex paraguariensis, and Calluna vulgaris. The intra-and interday precisions, stated as the relative standard deviation, were less than 2% and accuracy, based on relative error, was less than 10%. The limits of detection and quantification for arbutin and hydroquinone were 0.80 and 2.40 µg/mL and for the other compounds were 0.30 and 0.90 µg/mL, respectively. The methods developed can be used for routine quality control analysis of these effective compounds in different herbal slimming products.

<p>Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC₅₀ values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC₅₀ = 4.9 mg/mL). Grandivitinol (IC₅₀ = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species.</p><p><strong>Video Clip of Methodology</strong>:</p><p>8 min 26 sec:   <a href="https://www.youtube.com/v/x0gakPjgXmc">Full Screen</a>   <a href="https://www.youtube.com/watch?v=x0gakPjgXmc">Alternate</a></p><p> </p>

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Amaç: Bu çalışmanın amacı, farklı bitkisel zayıflama ürünlerinde arbutin ve hidrokinonun GC-MS yöntemiyle, eşzamanlı olarak belirlenmesi için basit, hassas ve spesifik bir yöntem geliştirmektir. Gereç ve Yöntemler: Türkiye'de bulunan dokuz bitkisel zayıflama ürününün sulu ve metanolik ekstreleri, arbutin ve hidrokinon analizi için GC-MS yöntemi ile değerlendirilmiştir. Bulgular: Arbutin ve hidrokinon için alıkonma zamanları sırasıyla 11.32 ve 5.44 dakika olarak bulunmuştur. Geliştirilen yöntemin doğrusallık aralığı arbutin ve hidrokinon için 5-500 ng/mL'dir. Kalite kontrol numunelerinden belirlenen ve bağıl standart sapma olarak ifade edilen gün-içi ve günlerarası kesinlik, arbutin ve hidrokinon için sırasıyla %1.94 ve %2.73'ten düşüktür. Bağıl hataya göre doğruluk değerleri arbutin ve hidrokinon için sırasıyla %1.13 ve %2.56 arasındadır. Gözlenebilme ve miktar tayin sınırı arbutin için sırasıyla 0.555 ve 1.665 ng/mL, hidrokinon için 0.031 ve 0.093 ng/mL'dir. Sonuç: Geliştirilen yöntem, farklı bitkisel zayıflama ürünlerinde arbutin ve hidrokinonun rutin kalite kontrol analizleri için kullanılabilir. Anahtar kelimeler: Arbutin, hidrokinon, GC-MS, bitkisel zayıflama ürünleri, Calluna vulgaris Objectives: The aim of our study was to develop a simple, precise, sensitive and specific method for the simultaneous determination of arbutin and hydroquinone in different herbal slimming products using GC-MS. Materials and Methods: The methanol and aqueous extracts of nine herbal slimming products in Turkey were evaluated for analysis of arbutin and hydroquinone using GC-MS method. Results: The retention times of arbutin and hydroquinone were found as 11.32 and 5.44 min, respectively. The linear ranges in this method were 5-500 ng/mL for arbutin and hydroquinone, respectively. The intra-and inter-day precisions, expressed as the relative standard deviation, were less than 1.94 and 2.73%, determined from quality control samples for arbutin and hydroquinone, and accuracy was within 1.13 and 2.56% in terms of relative error, respectively. The limit of detection and quantification were 0.555 and 1.665 ng/mL for arbutin, and 0.031 and 0.093 ng/mL for hydroquinone, respectively. Conclusion: The developed method can be used for routine quality control analysis of arbutin and hydroquinone in different herbal slimming products.