Dibohemamines A–C (5–7), three novel dimeric bohemamine analogs dimerized through a methylene group, were isolated from a marine-derived Streptomyces spinoverrucosus. The structures determined by spectroscopic analysis were confirmed through the semi-synthetic derivatization of monomeric bohemamines and formaldehyde. These reactions, which could occur under mild conditions, together with the detection of formaldehyde in the culture, revealed that this dimerization is a non-enzymatic process. In addition to the unique dimerization of the dibohemamines, dibohemamines B and C were found to have nM cytotoxicity against the non-small cell lung cancer cell line A549. In view of the potent cytotoxicity of compounds 6 and 7, a small library of bohemamine analogs w as generated for biological evaluation by utilizing a series of aryl and alkyl aldehydes.
Inducamides A–C (1–3),
three new chlorinated alkaloids featuring an amide skeleton generated
by a tryptophan fragment and a 6-methylsalicylic acid unit, were isolated
from a chemically induced mutant strain of Streptomyces sp. with the inducamides only being produced in the mutant strain.
Their structures, including stereochemistry, were determined by spectroscopic
analysis, Marfey’s method, and CD spectroscopy.
Two new 1,3-oxazin-6-one derivatives
(1 and 2) and six new bohemamine-type pyrrolizidine
alkaloids (3–8) were isolated from
the marine-derived Streptomyces spinoverrucosus strain
SNB-048. Their structures
including the absolute configurations were fully elucidated on the
basis of spectroscopic analysis, ECD spectra, quantum chemical calculations,
and chemical methods. Compounds 1 and 2 possess
a γ-lactam moiety and a 1,3-oxazin-6-one system.
The systematic screening of asymptomatic and pre-symptomatic individuals is a powerful tool for controlling community transmission of infectious disease on college campuses. Faced with a paucity of testing in the beginning of the COVID-19 pandemic, many universities developed molecular diagnostic laboratories focused on SARS-CoV-2 diagnostic testing on campus and in their broader communities. We established the UC Santa Cruz Molecular Diagnostic Lab in early April 2020 and began testing clinical samples just five weeks later. Using a clinically-validated laboratory developed test (LDT) that avoided supply chain constraints, an automated sample pooling and processing workflow, and a custom laboratory information management system (LIMS), we expanded testing from a handful of clinical samples per day to thousands per day with the testing capacity to screen our entire campus population twice per week. In this report we describe the technical, logistical, and regulatory processes that enabled our pop-up lab to scale testing and reporting capacity to thousands of tests per day.
Three new cyclohexene amine derivatives, daryamides D-F (1-3), a new arylamine derivative, carpatamide D (4), and a new ornithine lactamization derivative, ornilactam A (5), were isolated from the marine-derived Streptomyces strain SNE-011. Their structures, including absolute configurations, were elucidated on the basis of spectroscopic analysis and chemical methods. The carpatamide skeleton could be considered as the biosynthetic precursor of the daryamides.
Determining mechanism of action (MOA) is one of the biggest challenges in natural products discovery. Here, we report a comprehensive platform that uses Similarity Network Fusion (SNF) to improve MOA predictions by integrating data from the cytological profiling high-content imaging platform and the gene expression platform Functional Signature Ontology, and pairs these data with untargeted metabolomics analysis for de novo bioactive compound discovery. The predictive value of the integrative approach was assessed using a library of target-annotated small molecules as benchmarks. Using Kolmogorov–Smirnov (KS) tests to compare in-class to out-of-class similarity, we found that SNF retains the ability to identify significant in-class similarity across a diverse set of target classes, and could find target classes not detectable in either platform alone. This confirmed that integration of expression-based and image-based phenotypes can accurately report on MOA. Furthermore, we integrated untargeted metabolomics of complex natural product fractions with the SNF network to map biological signatures to specific metabolites. Three examples are presented where SNF coupled with metabolomics was used to directly functionally characterize natural products and accelerate identification of bioactive metabolites, including the discovery of the azoxy-containing biaryl compounds parkamycins A and B. Our results support SNF integration of multiple phenotypic screening approaches along with untargeted metabolomics as a powerful approach for advancing natural products drug discovery.
Determining mechanism of action (MOA) is one of the biggest challenges in natural products discovery. Here, we report a comprehensive platform that uses Similarity Network Fusion (SNF) to improve MOA predictions by integrating data from the cytological profiling high-content imaging platform and the gene expression platform FUSION. The predictive value of the integrative approach was assessed using a library of target-annotated small molecules as benchmarks. Using KS-tests to compare in-class to out-of-class similarity, we found that SNF resulted in improved power to correctly assign MOA over either dataset alone. Furthermore, we integrated untargeted metabolomics of complex natural product fractions to map biological signatures to specific metabolites. Three examples are presented where SNF coupled with metabolomics was used to directly functionally characterize natural products and accelerate identification of bioactive metabolites. Our results support SNF integration of multiple phenotypic screening approaches along with untargeted metabolomics as powerful approach for advancing natural products drug discovery.
Inducamides A-C, Chlorinated Alkaloids from an RNA Polymerase Mutant Strain of Streptomyces sp. -Inducamide A (Ia), B (Ib) and C (II) are shown to not exhibit significant antibacterial activity, but (II) reveals modest cytotoxicity. -(FU, P.; JAMISON, M.; LA, S.; MACMILLAN*, J. B.; Org. Lett. 16 (2014) 21, 5656-5659, http://dx.
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